Abstract
Aim: To screen and characterize pigeonpea genotypes using morpho-physiological, biochemical and molecular traits. Study Design: The field trial was conducted using the Randomized Block Design (RBD) while Completely Randomized Design was used for laboratory and pot screening experiments. Place and Duration of Study: The research trial was conducted at Laboratory and Pulse Research Farm, Model Bhitti, BAU, Sabour, which lies between 25°15’40” N latitude to 87°2’42” E longitude and 46 meters above sea level. Study was undertaken between July, 2018 to March, 2019. Methodology: Sixty pigeonpea genotypes were screened for submergence tolerance at seed stage in the laboratory. Based on results of laboratory screening, 40 genotypes with sufficient genotypic variability for waterlogging tolerance were further taken for seedling stage screening at field, finally 20 genotypes were taken to pots for waterlogging tolerance evaluation and characterization on the basis of morpho-physiological, biochemical and molecular traits. According to pot results, six contrasting genotypes were considered for RAPD primers amplification. Results: The three levels of sieving of genotypes fetched results directing the opportunity of particular genotypes to be sown inlow land areas. Character like seed colour varied from brown, dark brown to black, which showed significant relationship with level of tolerance. Significant (p=0.01) higher germination with less reduction due to waterlogging stress was observed in genotypes such as ICP-11809, ICPL-20098, NDA-1 and ICP-5028. Maximum survival percent was found in ICP-5028 (62.28%) while least survival percent was observed in ICP-7035 (10.98%). At the field stage, genotypes such as ICP-5028, ICPL-990985, ICPL-20238 were best performing genotypes. SPAD Chlorophyll Content results exhibited significant reduction among the susceptible genotypes. However, there was least reduction among tolerant genotypes such as LRG-30, Mal-9, Pusa-992 and ICP-5028.Genotypes namely: Manak, Pusa-991 and Pusa-992 faced hastened senescence under waterlogging condition as compared to ICP-5028, Mal-15, Mal-9, LRG-30. Molecular evaluation results of six genotypes chosen across screening showed that ICP-7035 and Manak were clubed together in one cluster. Nevertheless, ICP-5028 and Mal-9 were grouped in another cluster of Dendrogram, constructed using Jaccard similarity coefficient. In the present investigation two unique amplicons were amplified by primers OPA-13 and OPC-01. OPA-13 amplified unique band was linked with susceptible genotypes of size ~1240 bp while the unique amplicon given by OPC-01 was of ~980 bp size linked with tolerant genotypes. Conclusion: Available waterlogging tolerance in pigeonpea gene pool to some extent can provide source for breeding waterlogging tolerant cultivars. Physiological and genetic approach involving efficient screening techniques and evaluation of breeding material/lines under targeted environment for the traits linked to tolerance is likely to lead to the identification of specific component traits and high yielding varieties with improved stress tolerance.
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Claims 12
Claim · model-written summary
Seed colour was significantly related to the level of waterlogging tolerance in pigeonpea.
- Taxon
- Pigeonpea
Cajanus cajant0 - Organ / stage
- seed coloure1
- Outcome
- level of tolerancee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e1“Character like seed colour varied from brown, dark brown to black, which showed significant relationship with level of tolerance”
Claim · model-written summary
Germination of ICP-11809, ICPL-20098, NDA-1 and ICP-5028 was significantly higher under waterlogging stress, with less reduction.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- ICP-11809, ICPL-20098, NDA-1 and ICP-5028e2
- Treatment
- waterlogging stresse2
- Outcome
- germinatione2
- Direction
- highere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e2“Significant (p=0.01) higher germination with less reduction due to waterlogging stress was observed in genotypes such as ICP-11809, ICPL-20098, NDA-1 and ICP-5028”
Claim · model-written summary
Genotype ICP-5028 had the maximum survival percent (62.28%) under waterlogging.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- ICP-5028e3
- Outcome
- survival percente3
- Value
- 62.28%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e3“Maximum survival percent was found in ICP-5028 (62.28%)”
Claim · model-written summary
Genotype ICP-7035 had the least survival percent (10.98%) under waterlogging.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- ICP-7035e4
- Outcome
- survival percente4
- Value
- 10.98%e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e4“while least survival percent was observed in ICP-7035 (10.98%)”
Claim · model-written summary
SPAD chlorophyll content showed significant reduction among the susceptible genotypes under waterlogging.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- susceptible genotypese5
- Outcome
- SPAD Chlorophyll Contente5
- Direction
- reductione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e5“SPAD Chlorophyll Content results exhibited significant reduction among the susceptible genotypes”
Claim · model-written summary
SPAD chlorophyll content showed least reduction among tolerant genotypes LRG-30, Mal-9, Pusa-992 and ICP-5028.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- tolerant genotypes such as LRG-30, Mal-9, Pusa-992 and ICP-5028e6
- Outcome
- SPAD Chlorophyll Contente6
- Direction
- least reductione6
- Comparator
- susceptible genotypese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e6“SPAD Chlorophyll Content results exhibited significant reduction among the susceptible genotypes. However, there was least reduction among tolerant genotypes such as LRG-30, Mal-9, Pusa-992 and ICP-5028”
Claim · model-written summary
Manak, Pusa-991 and Pusa-992 showed hastened senescence under waterlogging compared with ICP-5028, Mal-15, Mal-9 and LRG-30.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- Manak, Pusa-991 and Pusa-992e7
- Treatment
- waterlogging conditione7
- Outcome
- senescencee7
- Direction
- hastenede7
- Comparator
- ICP-5028, Mal-15, Mal-9, LRG-30e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e7“Genotypes namely: Manak, Pusa-991 and Pusa-992 faced hastened senescence under waterlogging condition as compared to ICP-5028, Mal-15, Mal-9, LRG-30”
Claim · model-written summary
ICP-7035 and Manak clustered together in one molecular cluster.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- ICP-7035 and Manake1
- Outcome
- clubed together in one clustere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e1“ICP-7035 and Manak were clubed together in one cluster”
Claim · model-written summary
ICP-5028 and Mal-9 grouped in another cluster of the dendrogram.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- ICP-5028 and Mal-9e1
- Outcome
- grouped in another clustere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e1“ICP-5028 and Mal-9 were grouped in another cluster of Dendrogram”
Claim · model-written summary
Primer OPA-13 amplified a unique band of about 1240 bp linked with susceptible genotypes.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- susceptible genotypese1
- Outcome
- OPA-13 amplified unique bande1
- Value
- ~1240 bpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e1“OPA-13 amplified unique band was linked with susceptible genotypes of size ~1240 bp”
Claim · model-written summary
The unique amplicon of primer OPC-01 of about 980 bp was linked with tolerant genotypes.
- Taxon
- PigeonpeaCajanus cajant0
- Material
- tolerant genotypese1
- Outcome
- unique amplicon given by OPC-01e1
- Value
- ~980 bpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e1“the unique amplicon given by OPC-01 was of ~980 bp size linked with tolerant genotypes”
Claim · model-written summary
Pigeonpea gene pool can provide a source for breeding waterlogging-tolerant cultivars.
- Taxon
- PigeonpeaCajanus cajant0
- Outcome
- source for breeding waterlogging tolerant cultivarse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pigeonpea Genotypes”
e1“Available waterlogging tolerance in pigeonpea gene pool to some extent can provide source for breeding waterlogging tolerant cultivars”
The same questions in other papers
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.