Abstract
BACKGROUND: Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants, and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, Myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. RESULTS: In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. Besides, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of the MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits. MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity. CONCLUSIONS: These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of the MIOX gene family and their involvement in ascorbate biosynthesis in tomato.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Five tomato MIOX genes were identified.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- MIOXe0
- Outcome
- tomato genes encoding MIOX proteinse0
- Value
- fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified.”
Claim · model-written summary
Five MIOX genes differed in intron and exon patterns and were unevenly distributed over four chromosomes.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- MIOXe0
- Outcome
- different intron/exon patterns and unevenly distributed among four chromosomese0
- Value
- 5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes.”
t0“tomato”
Claim · model-written summary
SlMIOX genes showed expression in different plant tissues.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- different plant tissuese0
- Gene
- SlMIOXe0
- Outcome
- expression of SlMIOX genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues.”
t0“tomato”
Claim · model-written summary
MIOX4 overexpression significantly increased total ascorbate in leaves and red fruits versus control.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- overexpression linese0
- Outcome
- total ascorbate in leaves and red fruitse0
- Direction
- increasee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control.”
t0“tomato”
Claim · model-written summary
Increased AsA accumulation in MIOX4 lines was possibly due to multiple biosynthesis genes.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- MIOX4e0
- Outcome
- increased accumulation of AsAe0
- Qualifier
- multiple genes involved in AsA biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis.”
t0“tomato”
Claim · model-written summary
Myo inositol feeding implied improvement in AsA biosynthesis in leaf and fruit.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Myo inositol (MI) feedinge0
- Outcome
- AsA biosynthesis in leaves and fruitse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits.”
t0“tomato”
Claim · model-written summary
MIOX4 overexpression lines had better light response.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- MIOX4e0
- Outcome
- light responsee0
- Direction
- bettere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity.”
t0“tomato”
Claim · model-written summary
MIOX4 overexpression lines showed abiotic stress tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- MIOX4e0
- Outcome
- abiotic stress tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity.”
t0“tomato”
Claim · model-written summary
MIOX4 overexpression lines showed AsA biosynthesis capacity.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- MIOX4e0
- Outcome
- AsA biosynthesis capacitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity.”
t0“tomato”
The same questions in other papers
infection · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
14 papers in the same direction as this paper · 17 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.