Paper · 2020 · Ukrainian Journal of Ecology

Producing of potato varieties resistant to fusarial wilt by cell selection

N.A. Zakharchuk; M.M. Dolya; В. М. Положенець; et al.

4
claims
8
verbatim quotes
5
citations
2
research areas
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Open access

Abstract

The experiment has determined the characteristic features of callusogenesis, regeneration as well as of receiving resistant to fusariosis of potatoes cell lines in simulated conditions of biotic stress. The technologic elements of potato cell selection towards its resistance to Fusarium oxysporum and Fusarium sabucinum were presented. We succeeded in separating the lines which during a 3-year period of research were characterised by a higher resistance point as compared to the original varieties Gurman and Tyras. The plants-regenerants received on the selective medium were characterised by higher resistance (by 10–35%) to the pathogens action as compared to the regenerants of a control variant. The received lines were estimated on their yield capacity and starch content. The lines deviations on economically valuable indices either increased or decreased as to characteristic value. Herewith, the deviation upward is 25–75% depending on characteristics. The combination of maximal characteristic values was found in lines 12 and 28 of Gurman variety. The received lines were given to the selection-breeding plot for further testing. Key words: Potatoes; Culture in vitro; Cell selection; Selective medium; Phytotoxic metabolites; Plants-regenerants. References Behnke, �?. (1980). General resistance to late blight of Solanum tuberosum plants regenerated from callus resistant to culture filtrates of Phytophthora infestans. Theor. and Appl. Genet., 9, 151–152. Bolic, M., Foroughi-Wehr, B., & Kohler, F. (1986). In vitro selection for disease resistance in potato and barley. Nuclear Technigues and in vitro Culture for Plant Improvement, 12, 275–285. Kalashnikova, E.A. (2003). Cell selection of plants on their resistance to fungus diseases. Thesis of Doctoral Dissertation. Moscow (in Russian). Murashige, T. & Scoog, A. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497. Podgayetskiy, A.A. & Gorbas, S.B. (2013). The feasibility of getting high-starch forms from among the progeny from crossing of interspecific hybrids. Bulletin of Sumy National Agrarian University, 3(25), 224–228 Polozhenets, V.M., Tymoschuk, O.A. & Zhuravska, I.A. (2006). Fusarium dry rot of potatoe. Quarantine and plants protection, 8, 13–15. Zaharchuk, N.A. (2013). The effectiveness of potatoe selection in vitro on its resistance to Fusarium oxysporum and Fusarium sambucinum. Bulletin of Sumy National Agrarian University, 11 (26), 188–228.

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Claims 4

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Selected potato lines had a higher resistance point than the original varieties Gurman and Tyras over a 3-year period.

Material
Gurman and Tyrase0
Outcome
higher resistance pointe0
Direction
highere0
Comparator
original varietiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“We succeeded in separating the lines which during a 3-year period of research were characterised by a higher resistance point as compared to the original varieties Gurman and Tyras”

✓ located verbatim in the source · abstract, characters 337–517 · show in text ↑
t0

“potato varieties”

✓ located verbatim in the source · title, characters 13–29 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Regenerant plants from the selective medium had 10-35% higher resistance to the pathogens than regenerants of the control variant.

Treatment
selective mediume0
Interactor
pathogense0pathogen
Outcome
resistancee0
Direction
highere0
Value
10–35%e0
Comparator
regenerants of a control variante0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The plants-regenerants received on the selective medium were characterised by higher resistance (by 10–35%) to the pathogens action as compared to the regenerants of a control variant”

✓ located verbatim in the source · abstract, characters 519–702 · show in text ↑
t0

“potato varieties”

✓ located verbatim in the source · title, characters 13–29 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Deviations of the lines on economically valuable indices were upward by 25-75% depending on the characteristic.

Outcome
deviation upwarde0
Value
25–75%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The lines deviations on economically valuable indices either increased or decreased as to characteristic value. Herewith, the deviation upward is 25–75% depending on characteristics”

✓ located verbatim in the source · abstract, characters 782–963 · show in text ↑
t0

“potato varieties”

✓ located verbatim in the source · title, characters 13–29 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The combination of maximal characteristic values was found in lines 12 and 28 of the Gurman variety.

Material
lines 12 and 28 of Gurman varietye0
Outcome
combination of maximal characteristic valuese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The combination of maximal characteristic values was found in lines 12 and 28 of Gurman variety”

✓ located verbatim in the source · abstract, characters 965–1060 · show in text ↑
t0

“potato varieties”

✓ located verbatim in the source · title, characters 13–29 · show in text ↑

in vitro · disease · Solanum tuberosum (potato)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.