Abstract
Perception of pathogenic effectors in plants often relies on nucleotide-binding domain (NBS) and leucine-rich-repeat-containing (NLR) proteins. Some NLRs contain additional domains that function as integrated decoys for pathogen effector targets and activation of immune signalling. Wheat stripe rust is one of the most devastating diseases of crop plants. Here, we report the cloning of YrU1, a stripe rust resistance gene from the diploid wheat Triticum urartu, the progenitor of the A genome of hexaploid wheat. YrU1 encodes a coiled-coil-NBS-leucine-rich repeat protein with N-terminal ankyrin-repeat and C-terminal WRKY domains, representing a unique NLR structure in plants. Database searches identify similar architecture only in wheat relatives. Transient expression of YrU1 in Nicotiana benthamiana does not induce cell death in the absence of pathogens. The ankyrin-repeat and coiled-coil domains of YrU1 self-associate, suggesting that homodimerisation is critical for YrU1 function. The identification and cloning of this disease resistance gene sheds light on NLR protein function and may facilitate breeding to control the devastating wheat stripe rust disease.
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Claims 6
Claim · model-written summary
The stripe rust resistance gene YrU1 was cloned from the diploid wheat Triticum urartu.
- Taxon
- Triticum urartu
Triticum urartue1 - Gene
- YrU1e1
- Outcome
- cloning of YrU1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we report the cloning of YrU1, a stripe rust resistance gene from the diploid wheat Triticum urartu”
Claim · model-written summary
YrU1 encodes a coiled-coil-NBS-leucine-rich repeat protein with N-terminal ankyrin-repeat and C-terminal WRKY domains, a unique NLR structure in plants.
- Taxon
- Triticum urartuTriticum urartue1
- Gene
- YrU1e2
- Outcome
- coiled-coil-NBS-leucine-rich repeat protein with N-terminal ankyrin-repeat and C-terminal WRKY domainse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we report the cloning of YrU1, a stripe rust resistance gene from the diploid wheat Triticum urartu”
e2“YrU1 encodes a coiled-coil-NBS-leucine-rich repeat protein with N-terminal ankyrin-repeat and C-terminal WRKY domains, representing a unique NLR structure in plants”
Claim · model-written summary
Database searches identify a similar protein architecture only in wheat relatives.
Verbatim
e3“Database searches identify similar architecture only in wheat relatives”
Claim · model-written summary
Transient expression of YrU1 in Nicotiana benthamiana did not induce cell death in the absence of pathogens.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae4 - Treatment
- Transient expression of YrU1e4
- Outcome
- cell deathe4
- Direction
- does not inducee4
- Comparator
- in the absence of pathogense4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Transient expression of YrU1 in Nicotiana benthamiana does not induce cell death in the absence of pathogens”
Claim · model-written summary
The ankyrin-repeat and coiled-coil domains of YrU1 self-associate.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- YrU1e5
- Outcome
- self-associatee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The ankyrin-repeat and coiled-coil domains of YrU1 self-associate”
t0“immune receptor confers stripe rust resistance in wheat”
Claim · model-written summary
The authors suggest that homodimerisation is critical for YrU1 function.
- Taxon
- wheatTriticum aestivumt0
- Gene
- YrU1e6
- Outcome
- homodimerisation is critical for YrU1 functione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“suggesting that homodimerisation is critical for YrU1 function”
t0“immune receptor confers stripe rust resistance in wheat”
The same questions in other papers
transgenic · cytology · Nicotiana benthamianaPositive and negative results
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- positive
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