Abstract
Shoot tips of Potato (Solanum phureja) were cryopreserved with the encapsulation-dehydration method, including: coating of shoot tips in calcium alginate beads, pre-culture in a solution containing 0.75 M of sugar (0.1 M of sucrose +0.65 M of another cryoprotectant sugar), and dehydration (for 4.5 h) on silica gel to reduce water content to 21–27%. Regrowth of shoot tips was assessed after freezing in liquid nitrogen. After cryopreservation, coated shoot tips were cultivated on a medium enriched with growth substances to measure concentrations of soluble carbohydrates. Maltose and trehalose ensured a better cryopreservation (88% and 91% of regrowth, respectively). Regrowth rates of potato shoot tips in the presence of polyols were very low and no recovery was obtained after freezing of shoot tips that were pre-cultivated in monosaccharides. Concentration of soluble carbohydrates increased in shoot tips pre-cultivated in sucrose, trehalose or glucose, especially when sucrose was applied as the cryoprotectant. The application of sucrose helped potato shoot-tips to acquire a certain tolerance to dehydration and cryofreezing. The analysis of soluble carbohydrates in potato shoot tips confirmed the accumulation of sugars during the pre-culture process, principally in the form of sucrose. The statistical analysis showed that soluble carbohydrate contents in shoot tips increased when total carbohydrates increased in the cultivation medium. The modelling approach indicated that the contents of soluble carbohydrates increased when pre-cultivation of potato shoot-tips was conducted in sucrose media compared to the control and other sugars.
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Claims 11
Claim · model-written summary
Maltose pre-culture yielded 88% regrowth after cryopreservation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose and trehalose ensured a better cryopreservation (88% and 91% of regrowth, respectively).”
t0“potato (Solanum phureja)”
Claim · model-written summary
Trehalose pre-culture yielded 91% regrowth after cryopreservation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maltose and trehalose ensured a better cryopreservation (88% and 91% of regrowth, respectively).”
t0“potato (Solanum phureja)”
Claim · model-written summary
Polyol pre-culture produced very low regrowth rates in potato shoot tips.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- polyolse0
- Outcome
- Regrowth ratese0
- Direction
- very lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regrowth rates of potato shoot tips in the presence of polyols were very low”
Claim · model-written summary
Monosaccharide pre-culture produced no recovery after freezing.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- monosaccharidese0
- Outcome
- recoverye0
- Direction
- no recoverye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no recovery was obtained after freezing of shoot tips that were pre-cultivated in monosaccharides”
t0“potato (Solanum phureja)”
Claim · model-written summary
Sucrose pre-culture increased soluble carbohydrates in shoot tips.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- sucrosee0
- Outcome
- Concentration of soluble carbohydratese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concentration of soluble carbohydrates increased in shoot tips pre-cultivated in sucrose, trehalose or glucose, especially when sucrose was applied as the cryoprotectant.”
t0“potato (Solanum phureja)”
Claim · model-written summary
Trehalose pre-culture increased soluble carbohydrates in shoot tips.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- trehalosee0
- Outcome
- Concentration of soluble carbohydratese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concentration of soluble carbohydrates increased in shoot tips pre-cultivated in sucrose, trehalose or glucose”
t0“potato (Solanum phureja)”
Claim · model-written summary
Glucose pre-culture increased soluble carbohydrates in shoot tips.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- glucosee0
- Outcome
- Concentration of soluble carbohydratese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concentration of soluble carbohydrates increased in shoot tips pre-cultivated in sucrose, trehalose or glucose”
t0“potato (Solanum phureja)”
Claim · model-written summary
Sucrose application helped potato shoot tips acquire tolerance to dehydration and cryofreezing.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- application of sucrosee0
- Outcome
- tolerance to dehydration and cryofreezinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The application of sucrose helped potato shoot-tips to acquire a certain tolerance to dehydration and cryofreezing.”
Claim · model-written summary
Sugars accumulated during pre-culture, principally as sucrose.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- accumulation of sugarse0
- Qualifier
- principally in the form of sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The analysis of soluble carbohydrates in potato shoot tips confirmed the accumulation of sugars during the pre-culture process, principally in the form of sucrose.”
Claim · model-written summary
Shoot-tip soluble carbohydrates increased with total carbohydrates in cultivation medium.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- total carbohydrates increased in the cultivation mediume0
- Outcome
- soluble carbohydrate contents in shoot tipse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soluble carbohydrate contents in shoot tips increased when total carbohydrates increased in the cultivation medium”
t0“potato (Solanum phureja)”
Claim · model-written summary
The model indicated higher soluble carbohydrates after sucrose pre-culture than control and other sugars.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- sucrose mediae0
- Outcome
- contents of soluble carbohydratese0
- Direction
- increasede0
- Comparator
- the control and other sugarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The modelling approach indicated that the contents of soluble carbohydrates increased when pre-cultivation of potato shoot-tips was conducted in sucrose media compared to the control and other sugars.”
The same questions in other papers
maltose · growth · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
sucrose · quality: sugar starch · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
sucrose · disease · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
trehalose · growth · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
glucose · quality: sugar starch · Solanum tuberosum (potato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.