Abstract
Rates of ethylene production were determined in highly embryogenie and virtually non-embryogenic tissue cultures of Medicago sativa ssp.falcata during a 10-d induction period on medium containing 2,4,-dichlorophenoxyacetic acid and kinetin, and during the first 10 d of somatic embryo formation on growth regulator-free medium. In cultures of both genotypes, ethylene production increased rapidly, peaked after 10 d and then declined again rapidly. This fall in ethylene production rate also occurred when the cultures were kept on the growth regulator-containing medium, but it was more pronounced in cultures which were transferred to growth regulator-free medium, i.e. under conditions favouring somatic embryo formation. There were only minor differences in the rates of ethylene production between the two genotypes, mainly after transfer to growth regulator-free medium. Cobalt and nickel ions strongly inhibited ethylene biosynthesis throughout the culture period under investigation. They did not, however, affect embryo induction, but exerted strongly inhibitory effects on somatic embryo formation. Amino-oxyacetic acid and aminoethoxyvinylglycine had no effect on ethylene production during the inductive period. It is concluded from these experiments that the high rates of ethylene production during embryo induction are not essential for subsequent embryo differentiation.
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Claims 11
Claim · model-written summary
Ethylene production rose rapidly and peaked after ten days in both genotypes.
- Taxon
- Medicago sativa ssp.falcata
Medicago sativae1 - Outcome
- ethylene productione0
- Direction
- increased rapidlye0
- Value
- 10 de0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In cultures of both genotypes, ethylene production increased rapidly, peaked after 10 d”
e1“Medicago sativa ssp.falcata”
t0“Medicago sativa”
Claim · model-written summary
Ethylene production subsequently declined rapidly in both genotypes.
- Taxon
- Medicago sativaMedicago sativat0
- Outcome
- ethylene productione1
- Direction
- declined again rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and then declined again rapidly”
e1“This fall in ethylene production rate also occurred when the cultures were kept on the growth regulator-containing medium”
t0“Medicago sativa”
Claim · model-written summary
The fall in ethylene production was more pronounced after transfer to growth regulator free medium.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- transferred to growth regulator-free mediume0
- Outcome
- ethylene production ratee1
- Direction
- more pronouncede0
- Comparator
- growth regulator-containing mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was more pronounced in cultures which were transferred to growth regulator-free medium”
e1“This fall in ethylene production rate also occurred when the cultures were kept on the growth regulator-containing medium”
t0“Medicago sativa”
Claim · model-written summary
Ethylene production rates differed only slightly between the two genotypes.
- Taxon
- Medicago sativaMedicago sativat0
- Outcome
- rates of ethylene productione0
- Direction
- minor differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were only minor differences in the rates of ethylene production between the two genotypes, mainly after transfer to growth regulator-free medium”
t0“Medicago sativa”
Claim · model-written summary
Cobalt ions strongly inhibited ethylene biosynthesis.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- Cobalte0
- Outcome
- ethylene biosynthesise0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cobalt and nickel ions strongly inhibited ethylene biosynthesis throughout the culture period under investigation”
t0“Medicago sativa”
Claim · model-written summary
Nickel ions strongly inhibited ethylene biosynthesis.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- nickel ionse0
- Outcome
- ethylene biosynthesise0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cobalt and nickel ions strongly inhibited ethylene biosynthesis throughout the culture period under investigation”
t0“Medicago sativa”
Claim · model-written summary
Cobalt and nickel ions did not affect embryo induction.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- Cobalt and nickel ionse1
- Outcome
- embryo inductione0
- Direction
- did note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They did not, however, affect embryo induction”
e1“Cobalt and nickel ions”
t0“Medicago sativa”
Claim · model-written summary
Cobalt and nickel ions strongly inhibited somatic embryo formation.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- Cobalt and nickel ionse1
- Outcome
- somatic embryo formatione0
- Direction
- strongly inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but exerted strongly inhibitory effects on somatic embryo formation”
e1“Cobalt and nickel ions”
t0“Medicago sativa”
Claim · model-written summary
Amino oxyacetic acid had no effect on ethylene production during induction.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- Amino-oxyacetic acide0
- Outcome
- ethylene productione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amino-oxyacetic acid and aminoethoxyvinylglycine had no effect on ethylene production during the inductive period”
t0“Medicago sativa”
Claim · model-written summary
Aminoethoxyvinylglycine had no effect on ethylene production during induction.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- aminoethoxyvinylglycinee0
- Outcome
- ethylene productione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Amino-oxyacetic acid and aminoethoxyvinylglycine had no effect on ethylene production during the inductive period”
t0“Medicago sativa”
Claim · model-written summary
The authors concluded that high ethylene production during induction is not essential for later embryo differentiation.
- Taxon
- Medicago sativaMedicago sativat0
- Outcome
- subsequent embryo differentiatione0
- Qualifier
- high rates of ethylene production during embryo inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the high rates of ethylene production during embryo induction are not essential for subsequent embryo differentiation”
t0“Medicago sativa”
The same questions in other papers
cobalt atom · growth · Medicago sativa (alfalfa)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →(aminooxy)acetic acid · physiology: hormones · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.