Abstract
Heat stress induces misfolded protein accumulation in endoplasmic reticulum (ER), which initiates the unfolded protein response (UPR) in plants. Previous work has demonstrated the important role of a rice ER membrane-associated transcription factor OsbZIP74 (also known as OsbZIP50) in UPR. However, how OsbZIP74 and other membrane-associated transcription factors are involved in heat stress tolerance in rice is not reported. In the current study, we discovered that OsNTL3 is required for heat stress tolerance in rice. OsNTL3 is constitutively expressed and up-regulated by heat and ER stresses. OsNTL3 encodes a NAC transcription factor with a predicted C-terminal transmembrane domain. GFP-OsNTL3 relocates from plasma membrane to nucleus in response to heat stress and ER stress inducers. Loss-of-function mutation of OsNTL3 confers heat sensitivity while inducible expression of the truncated form of OsNTL3 without the transmembrane domain increases heat tolerance in rice seedlings. RNA-Seq analysis revealed that OsNTL3 regulates the expression of genes involved in ER protein folding and other processes. Interestingly, OsNTL3 directly binds to OsbZIP74 promoter and regulates its expression in response to heat stress. In turn, up-regulation of OsNTL3 by heat stress is dependent on OsbZIP74. Thus, our work reveals the important role of OsNTL3 in thermotolerance, and a regulatory circuit mediated by OsbZIP74 and OsNTL3 in communications among ER, plasma membrane and nucleus under heat stress conditions.
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Claims 9
Claim · model-written summary
OsNTL3 was required for rice heat tolerance.
- Taxon
- rice
Oryza sativae0 - Gene
- OsNTL3e0
- Outcome
- heat stress tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNTL3 is required for heat stress tolerance in rice”
Claim · model-written summary
OsNTL3 expression increased under heat and ER stress.
- Taxon
- riceOryza sativat0
- Treatment
- heat and ER stressese0
- Gene
- OsNTL3e0
- Outcome
- OsNTL3 is constitutively expressed and up-regulatede0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNTL3 is constitutively expressed and up-regulated by heat and ER stresses”
t0“rice”
Claim · model-written summary
OsNTL3 encodes a NAC transcription factor with a predicted C-terminal transmembrane domain.
- Taxon
- riceOryza sativat0
- Gene
- OsNTL3e0
- Outcome
- NAC transcription factor with a predicted C-terminal transmembrane domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNTL3 encodes a NAC transcription factor with a predicted C-terminal transmembrane domain”
t0“rice”
Claim · model-written summary
GFP-OsNTL3 moved from plasma membrane to nucleus after heat or ER stress induction.
- Taxon
- riceOryza sativat0
- Treatment
- heat stress and ER stress inducerse0
- Gene
- GFP-OsNTL3e0
- Outcome
- relocates from plasma membrane to nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GFP-OsNTL3 relocates from plasma membrane to nucleus in response to heat stress and ER stress inducers”
t0“rice”
Claim · model-written summary
OsNTL3 loss of function conferred heat sensitivity.
- Taxon
- riceOryza sativat0
- Treatment
- Loss-of-function mutation of OsNTL3e0
- Gene
- OsNTL3e0
- Outcome
- heat sensitivitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function mutation of OsNTL3 confers heat sensitivity”
t0“rice”
Claim · model-written summary
Induced truncated OsNTL3 expression increased seedling heat tolerance.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Treatment
- inducible expression of the truncated form of OsNTL3 without the transmembrane domaine0
- Gene
- OsNTL3e0
- Outcome
- heat tolerancee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inducible expression of the truncated form of OsNTL3 without the transmembrane domain increases heat tolerance in rice seedlings”
Claim · model-written summary
OsNTL3 regulated genes involved in ER protein folding and other processes.
- Taxon
- riceOryza sativat0
- Gene
- OsNTL3e0
- Outcome
- expression of genes involved in ER protein folding and other processese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNTL3 regulates the expression of genes involved in ER protein folding and other processes”
t0“rice”
Claim · model-written summary
OsNTL3 directly bound the OsbZIP74 promoter and regulated its expression after heat stress.
- Taxon
- riceOryza sativat0
- Treatment
- heat stresse0
- Gene
- OsNTL3e0
- Outcome
- OsbZIP74 promoter and regulates its expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNTL3 directly binds to OsbZIP74 promoter and regulates its expression in response to heat stress”
t0“rice”
Claim · model-written summary
Heat induction of OsNTL3 depended on OsbZIP74.
- Taxon
- riceOryza sativat0
- Treatment
- heat stresse0
- Gene
- OsbZIP74e0
- Outcome
- up-regulation of OsNTL3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulation of OsNTL3 by heat stress is dependent on OsbZIP74”
t0“rice”
The same questions in other papers
transgenic · tolerance: abiotic · Oryza sativa (rice)Positive and negative results
8 papers in the same direction as this paper · 1 in the opposite direction
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.