Abstract
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a tremendous threat to the production of wheat worldwide. The molecular mechanisms of Pst effectors that regulate wheat immunity are poorly understood. In this study, we identified an effector Pst18363 from Pst that suppresses plant cell death in Nicotiana benthamiana and in wheat. Knocking down Pst18363 expression by virus-mediated host-induced gene silencing significantly decreased the number of rust pustules, indicating that Pst18363 functions as an important pathogenicity factor in Pst. Pst18363 was proven to interact with wheat Nudix hydrolase 23 TaNUDX23. In wheat, silencing of TaNUDX23 by virus-induced gene silencing increased reactive oxygen species (ROS) accumulation induced by the avirulent Pst race CYR23, whereas overexpression of TaNUDX23 suppressed ROS accumulation induced by flg22 in Arabidopsis. In addition, TaNUDX23 suppressed Pst candidate effector Pst322-trigged cell death by decreasing ROS accumulation in N. benthamiana. Knocking down of TaNUDX23 expression attenuated Pst infection, indicating that TaNUDX23 is a negative regulator of defence. In N. benthamiana, Pst18363 stabilises TaNUDX23. Overall, our data suggest that Pst18363 stabilises TaNUDX23, which suppresses ROS accumulation to facilitate Pst infection.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Pst18363 suppressed cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- Pste0pathogen
- Gene
- Pst18363e0
- Outcome
- plant cell deathe0
- Direction
- suppressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst18363 from Pst that suppresses plant cell death in Nicotiana benthamiana and in wheat”
Claim · model-written summary
Pst18363 suppressed cell death in wheat.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Pste0pathogen
- Gene
- Pst18363e0
- Outcome
- plant cell deathe0
- Direction
- suppressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst18363 from Pst that suppresses plant cell death in Nicotiana benthamiana and in wheat”
Claim · model-written summary
Pst18363 interacted with wheat TaNUDX23.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pst18363e0pathogen
- Gene
- TaNUDX23e0
- Outcome
- Pst18363 was proven to interact with wheat Nudix hydrolase 23 TaNUDX23e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pst18363 was proven to interact with wheat Nudix hydrolase 23 TaNUDX23”
Claim · model-written summary
Silencing TaNUDX23 increased ROS accumulation induced by CYR23 in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- silencing of TaNUDX23 by virus-induced gene silencinge0
- Interactor
- Pst race CYR23e0pathogen
- Gene
- TaNUDX23e0
- Outcome
- reactive oxygen species (ROS) accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In wheat, silencing of TaNUDX23 by virus-induced gene silencing increased reactive oxygen species (ROS) accumulation induced by the avirulent Pst race CYR23”
Claim · model-written summary
TaNUDX23 overexpression suppressed flg22-induced ROS accumulation in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of TaNUDX23e0
- Gene
- TaNUDX23e0
- Outcome
- ROS accumulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of TaNUDX23 suppressed ROS accumulation induced by flg22 in Arabidopsis”
Claim · model-written summary
TaNUDX23 suppressed Pst322-triggered cell death in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- Pste0pathogen
- Gene
- TaNUDX23e0
- Outcome
- Pst candidate effector Pst322-trigged cell deathe0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNUDX23 suppressed Pst candidate effector Pst322-trigged cell death by decreasing ROS accumulation in N. benthamiana”
Claim · model-written summary
TaNUDX23 decreased ROS accumulation in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- Pste0pathogen
- Gene
- TaNUDX23e0
- Outcome
- ROS accumulatione0
- Direction
- decreasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNUDX23 suppressed Pst candidate effector Pst322-trigged cell death by decreasing ROS accumulation in N. benthamiana”
Claim · model-written summary
Silencing TaNUDX23 attenuated Pst infection.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knocking down of TaNUDX23 expressione0
- Interactor
- Pste0pathogen
- Gene
- TaNUDX23e0
- Outcome
- Pst infectione0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knocking down of TaNUDX23 expression attenuated Pst infection”
e1“production of wheat worldwide”
Claim · model-written summary
Pst18363 stabilised TaNUDX23 in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- Pst18363e0pathogen
- Gene
- TaNUDX23e0
- Outcome
- stabilises TaNUDX23e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In N. benthamiana, Pst18363 stabilises TaNUDX23”
The same questions in other papers
loss of function · physiology: oxidative · Triticum aestivum (wheat)Positive and negative results
5 papers in the same direction as this paper · 17 in the opposite direction
- negative
- positive
- negative
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.