Abstract
Perturbation of the cellular redox state by stress conditions is sensed by redox-sensitive proteins so that the cell can physiologically respond to stressors. However, the mechanisms linking sensing to response remain poorly understood in plants. Here we report that the transcription factor bZIP68 underwent in vivo oxidation in Arabidopsis cells under oxidative stress which is dependent on its redox-sensitive Cys320 residue. bZIP68 is primarily localized to the nucleus under normal growth conditions in Arabidopsis seedlings. Oxidative stress reduces its accumulation in the nucleus and increases its cytosolic localization. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) revealed that bZIP68 primarily binds to promoter regions containing the core G-box (CACGTG) or G-box-like motif of the genes involved in abiotic and biotic stress responses, photosynthesis, biosynthetic processes, and transcriptional regulation. The bzip68 mutant displayed slower growth under normal conditions but enhanced tolerance to oxidative stress. The results from the ChIP-seq and phenotypic and transcriptome comparison between the bzip68 mutant and wildtype indicate that bZIP68 normally suppresses expression of stress tolerance genes and promotes expression of growth-related genes, whereas its inactivation enhances stress tolerance but suppresses growth. bZIP68 might balance stress tolerance with growth through the extent of its oxidative inactivation according to the environment.
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Claims 12
Claim · model-written summary
bZIP68 underwent Cys320-dependent oxidation under oxidative stress in Arabidopsis cells.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- oxidative stresse0
- Gene
- bZIP68e0
- Outcome
- in vivo oxidatione0
- Qualifier
- Cys320 residuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription factor bZIP68 underwent in vivo oxidation in Arabidopsis cells under oxidative stress which is dependent on its redox-sensitive Cys320 residue.”
Claim · model-written summary
bZIP68 was primarily nuclear under normal growth conditions in Arabidopsis seedlings.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- seedlingse0
- Gene
- bZIP68e0
- Outcome
- localized to the nucleuse0
- Qualifier
- normal growth conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bZIP68 is primarily localized to the nucleus under normal growth conditions in Arabidopsis seedlings.”
Claim · model-written summary
Oxidative stress reduced nuclear accumulation of bZIP68.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Oxidative stresse0
- Gene
- bZIP68t0
- Outcome
- accumulation in the nucleuse0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oxidative stress reduces its accumulation in the nucleus”
t0“bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis”
Claim · model-written summary
Oxidative stress increased cytosolic localization of bZIP68.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Oxidative stresse0
- Gene
- bZIP68t0
- Outcome
- cytosolic localizatione0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oxidative stress reduces its accumulation in the nucleus and increases its cytosolic localization.”
t0“bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis”
Claim · model-written summary
bZIP68 bound primarily to promoters with G-box or G-box-like motifs of genes involved in stress responses, photosynthesis, biosynthesis, and transcriptional regulation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bZIP68e0
- Outcome
- binds to promoter regions containing the core G-box (CACGTG) or G-box-like motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bZIP68 primarily binds to promoter regions containing the core G-box (CACGTG) or G-box-like motif of the genes involved in abiotic and biotic stress responses, photosynthesis, biosynthetic processes, and transcriptional regulation.”
t0“Arabidopsis”
Claim · model-written summary
The bzip68 mutant grew more slowly than wildtype under normal conditions.
- Taxon
- ArabidopsisArabidopsist0
- Material
- bzip68 mutante0
- Outcome
- growthe0
- Direction
- slowere0
- Qualifier
- normal conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bzip68 mutant displayed slower growth under normal conditions but enhanced tolerance to oxidative stress.”
t0“Arabidopsis”
Claim · model-written summary
The bzip68 mutant had enhanced oxidative-stress tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Material
- bzip68 mutante0
- Outcome
- tolerance to oxidative stresse0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bzip68 mutant displayed slower growth under normal conditions but enhanced tolerance to oxidative stress.”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that bZIP68 normally suppresses stress-tolerance gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bZIP68e0
- Outcome
- expression of stress tolerance genese0
- Direction
- suppressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bZIP68 normally suppresses expression of stress tolerance genes”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that bZIP68 normally promotes growth-related gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bZIP68t0
- Outcome
- expression of growth-related genese0
- Direction
- promotese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promotes expression of growth-related genes”
t0“bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis”
Claim · model-written summary
The authors infer that bZIP68 inactivation enhances stress tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bZIP68t0
- Outcome
- stress tolerancee0
- Direction
- enhancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its inactivation enhances stress tolerance”
t0“bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis”
Claim · model-written summary
The authors infer that bZIP68 inactivation suppresses growth.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bZIP68t0
- Outcome
- growthe0
- Direction
- suppressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its inactivation enhances stress tolerance but suppresses growth”
t0“bZIP68 plays a role in balancing stress tolerance with growth in Arabidopsis”
Claim · model-written summary
The authors hypothesize that oxidative inactivation of bZIP68 balances stress tolerance and growth according to environment.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bZIP68e0
- Outcome
- balance stress tolerance with growthe0
- Qualifier
- the extent of its oxidative inactivation according to the environmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bZIP68 might balance stress tolerance with growth through the extent of its oxidative inactivation according to the environment.”
t0“Arabidopsis”
The same questions in other papers
oxidative · molecular: metabolites · ArabidopsisResults agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.