Abstract
Beneficial Pseudomonas spp. produce an array of antimicrobial secondary metabolites such as cyclic lipopeptides (CLPs). We investigated the capacity of CLP-producing Pseudomonas strains and their crude CLP extracts to control rice blast caused by Magnaporthe oryzae, both in a direct manner and via induced systemic resistance (ISR). In planta biocontrol assays showed that lokisin-, white line inducing principle (WLIP)-, entolysin- and N3-producing strains successfully induced resistance to M. oryzae VT5M1. Furthermore, crude extracts of lokisin, WLIP and entolysin gave similar ISR results when tested in planta. In contrast, a xantholysin-producing strain and crude extracts of N3, xantholysin and orfamide did not induce resistance against the rice blast disease. The role of WLIP in triggering ISR was further confirmed by using WLIP-deficient mutants. The severity of rice blast disease was significantly reduced when M. oryzae spores were pre-treated with crude extracts of N3, lokisin, WLIP, entolysin or orfamide prior to inoculation. In vitro microscopic assays further revealed the capacity of crude N3, lokisin, WLIP, entolysin, xantholysin and orfamide to significantly inhibit appressoria formation by M. oryzae. In addition, the lokisin and WLIP biosynthetic gene clusters in the producing strains are described. In short, our study demonstrates the biological activity of structurally diverse CLPs in the control of the rice blast disease caused by M. oryzae. Furthermore, we provide insight into the non-ribosomal peptide synthetase genes encoding the WLIP and lokisin biosynthetic machineries..
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Claims 11
Claim · model-written summary
Lokisin-producing strains induced resistance to M. oryzae VT5M1.
- Taxon
- Rice
Oryza sativat0 - Treatment
- lokisin-e0
- Interactor
- M. oryzae VT5M1
Pyricularia oryzaee0pathogene1 - Outcome
- resistance to M. oryzae VT5M1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lokisin-, white line inducing principle (WLIP)-, entolysin- and N3-producing strains successfully induced resistance to M. oryzae VT5M1”
t0“Rice”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
WLIP-producing strains induced resistance to M. oryzae VT5M1.
- Taxon
- RiceOryza sativat0
- Treatment
- white line inducing principle (WLIP)-e0
- Interactor
- M. oryzae VT5M1Pyricularia oryzaee0pathogene1
- Outcome
- resistance to M. oryzae VT5M1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“white line inducing principle (WLIP)-, entolysin- and N3-producing strains successfully induced resistance to M. oryzae VT5M1”
t0“Rice”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
Entolysin-producing strains induced resistance to M. oryzae VT5M1.
- Taxon
- RiceOryza sativat0
- Treatment
- entolysin-e0
- Interactor
- M. oryzae VT5M1Pyricularia oryzaee0pathogene1
- Outcome
- resistance to M. oryzae VT5M1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“entolysin- and N3-producing strains successfully induced resistance to M. oryzae VT5M1”
t0“Rice”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
N3-producing strains induced resistance to M. oryzae VT5M1.
- Taxon
- RiceOryza sativat0
- Treatment
- N3-producing strainse0
- Interactor
- M. oryzae VT5M1Pyricularia oryzaee0pathogene1
- Outcome
- resistance to M. oryzae VT5M1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N3-producing strains successfully induced resistance to M. oryzae VT5M1”
t0“Rice”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
Crude lokisin, WLIP and entolysin extracts gave similar induced resistance results.
- Taxon
- RiceOryza sativat0
- Treatment
- crude extracts of lokisin, WLIP and entolysine0
- Outcome
- ISR resultse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crude extracts of lokisin, WLIP and entolysin gave similar ISR results when tested in planta”
t0“Rice”
Claim · model-written summary
Xantholysin-producing strain did not induce rice blast resistance.
- Taxon
- riceOryza sativae1
- Treatment
- a xantholysin-producing straine0
- Interactor
- Magnaporthe oryzaePyricularia oryzaee1pathogen
- Outcome
- resistance against the rice blast diseasee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a xantholysin-producing strain and crude extracts of N3, xantholysin and orfamide did not induce resistance against the rice blast disease”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
Crude N3, xantholysin and orfamide extracts did not induce rice blast resistance.
- Taxon
- riceOryza sativae1
- Treatment
- crude extracts of N3, xantholysin and orfamidee0
- Interactor
- Magnaporthe oryzaePyricularia oryzaee1pathogen
- Outcome
- resistance against the rice blast diseasee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crude extracts of N3, xantholysin and orfamide did not induce resistance against the rice blast disease”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
WLIP-deficient mutants confirmed a role for WLIP in induced resistance.
- Taxon
- RiceOryza sativat0
- Outcome
- role of WLIP in triggering ISRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The role of WLIP in triggering ISR was further confirmed by using WLIP-deficient mutants”
t0“Rice”
Claim · model-written summary
Pretreatment of spores with N3, lokisin, WLIP, entolysin or orfamide extracts significantly reduced rice blast severity.
- Taxon
- RiceOryza sativat0
- Treatment
- pre-treated with crude extracts of N3, lokisin, WLIP, entolysin or orfamidee0
- Interactor
- M. oryzaePyricularia oryzaee0pathogene1
- Outcome
- severity of rice blast diseasee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“severity of rice blast disease was significantly reduced when M. oryzae spores were pre-treated with crude extracts of N3, lokisin, WLIP, entolysin or orfamide prior to inoculation”
t0“Rice”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
N3, lokisin, WLIP, entolysin, xantholysin and orfamide inhibited M. oryzae appressoria formation in vitro.
- Taxon
- RiceOryza sativat0
- Treatment
- crude N3, lokisin, WLIP, entolysin, xantholysin and orfamidee0
- Interactor
- M. oryzaePyricularia oryzaee0pathogene1
- Outcome
- appressoria formation by M. oryzaee0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crude N3, lokisin, WLIP, entolysin, xantholysin and orfamide to significantly inhibit appressoria formation by M. oryzae”
t0“Rice”
e1“rice blast caused by Magnaporthe oryzae”
Claim · model-written summary
Lokisin and WLIP biosynthetic gene clusters were described.
- Taxon
- RiceOryza sativat0
- Outcome
- lokisin and WLIP biosynthetic gene clusterse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lokisin and WLIP biosynthetic gene clusters in the producing strains are described”
t0“Rice”
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