Abstract
Copper is an essential nutrient for symbiotic nitrogen fixation. This element is delivered by the host plant to the nodule, where membrane copper (Cu) transporter would introduce it into the cell to synthesize cupro-proteins. COPT family members in the model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy and phenotypical characterization of a Tnt1 insertional mutant line were carried out in the nodule-specific M. truncatula COPT family member. Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. It is located in the plasma membrane of the differentiation, interzone and early fixation zones. Loss of MtCOPT1 function results in a Cu-mitigated reduction of biomass production when the plant obtains its nitrogen exclusively from symbiotic nitrogen fixation. Mutation of MtCOPT1 results in diminished nitrogenase activity in nodules, likely an indirect effect from the loss of a Cu-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. These data are consistent with a model in which MtCOPT1 transports Cu from the apoplast into nodule cells to provide Cu for essential metabolic processes associated with symbiotic nitrogen fixation.
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Claims 7
Claim · model-written summary
MtCOPT1 is the only nodule-specific COPT gene in Medicago truncatula.
- Taxon
- Medicago truncatula
Medicago truncatulat0 - Gene
- MtCOPT1e1
- Outcome
- nodule-specific COPT genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e1“MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene”
Claim · model-written summary
MtCOPT1 is located in the plasma membrane of the differentiation, interzone and early fixation zones of nodules.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Organ / stage
- differentiation, interzone and early fixation zonese2
- Gene
- MtCOPT1e1
- Outcome
- located in the plasma membranee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e1“MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene”
e2“It is located in the plasma membrane of the differentiation, interzone and early fixation zones”
Claim · model-written summary
Loss of MtCOPT1 function reduced biomass production, in a Cu-mitigated way, when nitrogen came only from symbiotic fixation.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Treatment
- Loss of MtCOPT1 functione3
- Gene
- MtCOPT1e3
- Outcome
- biomass productione3
- Direction
- reductione3
- Qualifier
- nitrogen exclusively from symbiotic nitrogen fixatione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e3“Loss of MtCOPT1 function results in a Cu-mitigated reduction of biomass production when the plant obtains its nitrogen exclusively from symbiotic nitrogen fixation”
Claim · model-written summary
Mutation of MtCOPT1 diminished nitrogenase activity in nodules.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Organ / stage
- nodulese4
- Treatment
- Mutation of MtCOPT1e4
- Outcome
- nitrogenase activitye4
- Direction
- diminishede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e4“Mutation of MtCOPT1 results in diminished nitrogenase activity in nodules”
Claim · model-written summary
The reduced nitrogenase activity is likely an indirect effect of losing a copper-dependent function.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Outcome
- nitrogenase activitye4
- Qualifier
- likely an indirect effect from the loss of a Cu-dependent functione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e4“Mutation of MtCOPT1 results in diminished nitrogenase activity in nodules”
e5“likely an indirect effect from the loss of a Cu-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids”
Claim · model-written summary
MtCOPT1 transports copper from the apoplast into nodule cells.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Gene
- MtCOPT1e6
- Outcome
- transports Cu from the apoplast into nodule cellse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e6“MtCOPT1 transports Cu from the apoplast into nodule cells”
Claim · model-written summary
The Medicago truncatula genome encodes eight COPT transporters.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Outcome
- COPT transporterse7
- Value
- eighte7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Medicago truncatula”
e7“Medicago truncatula genome encodes eight COPT transporters”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.