Abstract
BACKGROUND: Coconut genetic resources are threatened by pests and pathogens, natural hazards and human activities. Cryopreservation is the only method allowing the safe and cost-effective long-term conservation of recalcitrant seed species such as coconut. OBJECTIVE: The objective of this work was to test the effect of cryopreservation and of cryostorage duration on coconut pollen germination and fertility. MATERIALS AND METHODS: Pollen of two coconut varieties (West Coast Tall WWCTW and Chowghat Orange Dwarf CODC) was collected in March-May over three successive years, desiccated to 7.5 % moisture content (FW) and cryopreserved by direct immersion in liquid nitrogen. RESULTS: Germination and pollen tube length (PTL) of desiccated and cryopreserved pollen were not significantly different for both WCT and COD over the three harvest months of the three consecutive years of study. Pollen germination ranged from 24 to 32 % in desiccated pollen whereas it was between 26 and 29 % in cryopreserved COD pollen. In the case of WCT, germination ranged from 30 to 31 % in desiccated pollen, while it was between 28 and 32 % in cryopreserved pollen. PTL of cryopreserved pollen ranged between 224-390 nm and 226-396 mm for COD and WCT, respectively. Germination of COD pollen varied between 29.0 and 44.1 % after 4 years and 1.0/1.5 years cryostorage, respectively. Germination of WCT pollen did not change significantly between 0 and 6 years cryostorage, being comprised between 32 (24 h) and 40 % (1.5 years). Germination and vigour of cryopreserved pollen were generally higher compared to that of pollen dried in oven and non-cryopreserved. Normal seed set was observed in COD and WCT palms using pollen cryostored for 6 months and 4 years. Cryopreserved pollen of five Tall and five Dwarf accessions displayed 24-31 % and 25-49 % germination, respectively. CONCLUSION: These results show that it is now possible to establish pollen cryobanks to contribute to coconut germplasm long-term conservation.
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Claims 17
Claim · model-written summary
Desiccated and cryopreserved coconut pollen did not differ significantly in germination across harvest months and study years.
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Germination and pollen tube length (PTL) of desiccated and cryopreserved pollen were not significantly different for both WCT and COD over the three harvest months of the three consecutive years of study.”
Claim · model-written summary
Desiccated and cryopreserved coconut pollen did not differ significantly in pollen tube length across harvest months and study years.
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Germination and pollen tube length (PTL) of desiccated and cryopreserved pollen were not significantly different for both WCT and COD over the three harvest months of the three consecutive years of study.”
Claim · model-written summary
Desiccated COD coconut pollen germination ranged from 24 to 32 %.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Pollen germination ranged from 24 to 32 % in desiccated pollen whereas it was between 26 and 29 % in cryopreserved COD pollen.”
Claim · model-written summary
Cryopreserved COD coconut pollen germination was between 26 and 29 %.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Pollen germination ranged from 24 to 32 % in desiccated pollen whereas it was between 26 and 29 % in cryopreserved COD pollen.”
Claim · model-written summary
Desiccated WCT coconut pollen germination ranged from 30 to 31 %.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“In the case of WCT, germination ranged from 30 to 31 % in desiccated pollen, while it was between 28 and 32 % in cryopreserved pollen.”
Claim · model-written summary
Cryopreserved WCT coconut pollen germination was between 28 and 32 %.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“In the case of WCT, germination ranged from 30 to 31 % in desiccated pollen, while it was between 28 and 32 % in cryopreserved pollen.”
Claim · model-written summary
Cryopreserved COD coconut pollen tube length ranged between 224-390 nm.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“PTL of cryopreserved pollen ranged between 224-390 nm and 226-396 mm for COD and WCT, respectively.”
Claim · model-written summary
Cryopreserved WCT coconut pollen tube length ranged between 226-396 mm.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“PTL of cryopreserved pollen ranged between 224-390 nm and 226-396 mm for COD and WCT, respectively.”
Claim · model-written summary
COD coconut pollen germination varied between 29.0 and 44.1 % after 4 years and 1.0/1.5 years of cryostorage, respectively.
- Taxon
- Coconutt0
- Material
- CODe0
- Outcome
- Germinatione0
- Value
- 29.0 and 44.1 %e0
- Qualifier
- after 4 years and 1.0/1.5 years cryostoragee0
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Germination of COD pollen varied between 29.0 and 44.1 % after 4 years and 1.0/1.5 years cryostorage, respectively.”
Claim · model-written summary
WCT coconut pollen germination did not change significantly across 0 to 6 years of cryostorage, ranging from 32 to 40 %.
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Germination of WCT pollen did not change significantly between 0 and 6 years cryostorage, being comprised between 32 (24 h) and 40 % (1.5 years).”
Claim · model-written summary
Cryopreserved coconut pollen generally had higher germination than pollen dried in an oven without cryopreservation.
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Germination and vigour of cryopreserved pollen were generally higher compared to that of pollen dried in oven and non-cryopreserved.”
Claim · model-written summary
Cryopreserved coconut pollen generally had higher vigour than pollen dried in an oven without cryopreservation.
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Germination and vigour of cryopreserved pollen were generally higher compared to that of pollen dried in oven and non-cryopreserved.”
Claim · model-written summary
COD coconut palms had normal seed set using pollen cryostored for 6 months and 4 years.
- Taxon
- Coconutt0
- Material
- CODe0
- Treatment
- pollen cryostored for 6 months and 4 yearse0
- Outcome
- Normal seed sete0
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Normal seed set was observed in COD and WCT palms using pollen cryostored for 6 months and 4 years.”
Claim · model-written summary
WCT coconut palms had normal seed set using pollen cryostored for 6 months and 4 years.
- Taxon
- Coconutt0
- Material
- WCTe0
- Treatment
- pollen cryostored for 6 months and 4 yearse0
- Outcome
- Normal seed sete0
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Normal seed set was observed in COD and WCT palms using pollen cryostored for 6 months and 4 years.”
Claim · model-written summary
Cryopreserved coconut pollen from five Tall accessions germinated at 24-31 %.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Cryopreserved pollen of five Tall and five Dwarf accessions displayed 24-31 % and 25-49 % germination, respectively.”
Claim · model-written summary
Cryopreserved coconut pollen from five Dwarf accessions germinated at 25-49 %.
Not reportedDirection
Verbatim
t0“Coconut (Cocos nucifera l.) pollen cryopreservation.”
e0“Cryopreserved pollen of five Tall and five Dwarf accessions displayed 24-31 % and 25-49 % germination, respectively.”
Claim · model-written summary
The authors conclude that coconut pollen cryobanks can now be established for long-term germplasm conservation.
Verbatim
e0“it is now possible to establish pollen cryobanks to contribute to coconut germplasm long-term conservation.”
The same questions in other papers
in vitro · growth · Cocos nucifera (coconut palm)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.