Abstract
Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.
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Claims 9
Claim · model-written summary
Simultaneous inoculation produced a larger productivity gain in K-8274 than K-3358.
- Taxon
- pea
Lathyrus oleraceuse0 - Material
- K-8274e0
- Treatment
- simultaneous inoculation with Rh and AMe0
- Interactor
- Rhe0symbiont
- Second interactor
- AMe0symbiont
- Outcome
- productivity gaine0
- Direction
- highere0
- Comparator
- K-3358e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358”
Claim · model-written summary
The two pea lines had 111 differentially expressed seed proteins.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- seede1
- Outcome
- differentially expressed proteinse0
- Value
- 111e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identify 111 differentially expressed proteins in the two lines”
e1“seed maturation”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
Inoculated K-8274 showed prolonged seed maturation.
- Taxon
- PeaLathyrus oleraceust0
- Material
- K-8274e0
- Treatment
- inoculatione0
- Outcome
- seed maturatione0
- Direction
- prolongatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
Inoculated K-8274 showed up regulation of respiration and protein biosynthesis proteins.
- Taxon
- PeaLathyrus oleraceust0
- Material
- K-8274e0
- Treatment
- inoculatione0
- Outcome
- proteins involved in cellular respiration, protein biosynthesise0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
Inoculated K-8274 showed down regulation of LEA proteins.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- late-embryogenesis abundant (LEA) proteinse0
- Direction
- down-regulatione0
Not reportedMaterialTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of late-embryogenesis abundant (LEA) proteins”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
K-3358 had lower levels of proteins related to cell metabolism.
- Taxon
- PeaLathyrus oleraceust0
- Material
- K-3358e0
- Outcome
- proteins, related to cell metabolisme0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
The authors propose that symbiotic responsiveness is linked to prolonged seed filling.
- Taxon
- PeaLathyrus oleraceust0
- Material
- EIBSM traite0
- Outcome
- seed fillinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we propose that the EIBSM trait is linked to prolongation of seed filling”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
The authors recommend considering prolonged seed filling in pulse breeding.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- needs to be taken into account in pulse crop breeding programse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“prolongation of seed filling that needs to be taken into account in pulse crop breeding programs”
t0“Pea (Pisum sativum L.)”
Claim · model-written summary
The raw proteome data were deposited under PXD013479.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- raw datae0
- Value
- PXD013479e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The raw data have been deposited to the ProteomeXchange with identifier PXD013479”
t0“Pea (Pisum sativum L.)”
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.