Abstract
(RdRs) that play major roles in the defense against RNA viruses have been identified: (i) RdR1, which is responsible for the viral small RNAs (vsRNAs) found in virus-infected plants, and, (ii) RdR6, which acts as a surrogate in the absence of RdR1. In this study, the role of RdR6 in the defense against viroid infection was examined by knock-down of RdR6 followed by potato spindle tuber viroid (PSTVd) infection. The suppression of RdR6 expression increased the plant's growth, as was illustrated by the plant's increased height. PSTVd infection of RdR6 compromised plants resulted in an approximately three-fold increase in the accumulation of viroid RNA as compared to that seen in control plants. Additionally, RNA gel blot assay revealed an increase in the number of viroids derived small RNAs in RdR6 suppressed plants as compared to control plants. These data provide a direct correlation between RdR6 and viroid accumulation and indicate the role of RDR6 in the plant's susceptibility to viroid infection.
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Claims 3
Claim · model-written summary
RdR6 suppression increased plant height.
- Taxon
- Nicotiana Benthamiana
Nicotiana benthamianat0 - Treatment
- suppression of RdR6 expressione0
- Gene
- RdR6e0
- Outcome
- heighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of RdR6 expression increased the plant's growth, as was illustrated by the plant's increased height.”
t0“Nicotiana Benthamiana”
Claim · model-written summary
PSTVd accumulation rose about threefold in RdR6 compromised plants compared with controls.
- Taxon
- Nicotiana BenthamianaNicotiana benthamianat0
- Treatment
- RdR6 compromisede0
- Interactor
- PSTVde0pathogen
- Gene
- RdR6e0
- Outcome
- accumulation of viroid RNAe0
- Direction
- increasee0
- Value
- approximately three-folde0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PSTVd infection of RdR6 compromised plants resulted in an approximately three-fold increase in the accumulation of viroid RNA as compared to that seen in control plants.”
t0“Nicotiana Benthamiana”
Claim · model-written summary
Viroid-derived small RNAs increased in RdR6 suppressed plants compared with controls.
- Taxon
- Nicotiana BenthamianaNicotiana benthamianat0
- Treatment
- RdR6 suppressede0
- Interactor
- viroidse0pathogen
- Gene
- RdR6e0
- Outcome
- number of viroids derived small RNAse0
- Direction
- increasee0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA gel blot assay revealed an increase in the number of viroids derived small RNAs in RdR6 suppressed plants as compared to control plants.”
t0“Nicotiana Benthamiana”
The same questions in other papers
loss of function · growth · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 17 in the opposite direction
- negative
- negative
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.