Abstract
Heat stress during Brassica napus seed filling severely impairs yield and oil content. However, the mechanisms underlying heat-stress effects on B. napus seed photosynthesis and oil accumulation remain elusive. In this study, we showed that heat stress resulted in reduction of seed oil accumulation, whereas the seed sugar content was enhanced, which indicated that incorporation of carbohydrates into triacylglycerols was impaired. Photosynthesis and respiration rates, and the maximum quantum yield of photosystem II in developing seeds were inhibited by heat stress. Transcriptome analysis revealed that heat stress led to up-regulation of genes associated with high light response, providing evidence that photoinhibition was induced by heat stress. BnWRI1 and its downstream genes, including genes involved in de novo fatty acid biosynthesis pathway, were down-regulated by heat stress. Overexpression of BnWRI1 with a seed-specific promoter stabilized both oil accumulation and photosynthesis under the heat-stress condition, which suggested BnWRI1 plays an important role in mediating the effect of heat stress on fatty acid biosynthesis. A number of sugar transporter genes were inhibited by heat stress, resulting in defective integration of carbohydrates into triacylglycerols units. The results collectively demonstrated that disturbances of the seed photosynthesis machinery, impairment of carbohydrates incorporation into triacylglycerols and transcriptional deregulation of the BnWRI1 pathway by heat stress might be the major cause of decreased oil accumulation in the seed.
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Claims 13
Claim · model-written summary
Heat stress reduced Brassica napus seed oil accumulation.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Treatment
- heat stresse0
- Outcome
- seed oil accumulatione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“heat stress resulted in reduction of seed oil accumulation”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress enhanced seed sugar content.
- Taxon
- Brassica napusBrassica napuse2
- Treatment
- heat stresse1
- Outcome
- seed sugar contente0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seed sugar content was enhanced”
e1“heat stress resulted in reduction of seed oil accumulation, whereas the seed sugar content was enhanced”
e2“Brassica napus seed filling”
Claim · model-written summary
Heat stress inhibited photosynthesis rates in developing seeds.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- developing seedse0
- Treatment
- heat stresse0
- Outcome
- Photosynthesise0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photosynthesis and respiration rates, and the maximum quantum yield of photosystem II in developing seeds were inhibited by heat stress”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress inhibited respiration rates in developing seeds.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- developing seedse0
- Treatment
- heat stresse0
- Outcome
- respiration ratese0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photosynthesis and respiration rates, and the maximum quantum yield of photosystem II in developing seeds were inhibited by heat stress”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress inhibited maximum quantum yield of photosystem II in developing seeds.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- developing seedse0
- Treatment
- heat stresse0
- Outcome
- maximum quantum yield of photosystem IIe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photosynthesis and respiration rates, and the maximum quantum yield of photosystem II in developing seeds were inhibited by heat stress”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress up-regulated high-light-response genes.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- heat stresse0
- Outcome
- genes associated with high light responsee0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heat stress led to up-regulation of genes associated with high light response”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress down-regulated BnWRI1.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- heat stresse0
- Gene
- BnWRI1e0
- Outcome
- BnWRI1e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnWRI1 and its downstream genes, including genes involved in de novo fatty acid biosynthesis pathway, were down-regulated by heat stress”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress down-regulated BnWRI1 downstream genes including fatty-acid biosynthesis genes.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- heat stresse0
- Gene
- downstream genese0
- Outcome
- genes involved in de novo fatty acid biosynthesis pathwaye0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnWRI1 and its downstream genes, including genes involved in de novo fatty acid biosynthesis pathway, were down-regulated by heat stress”
e1“Brassica napus seed filling”
Claim · model-written summary
Seed-specific BnWRI1 overexpression stabilized oil accumulation under heat stress.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- Overexpression of BnWRI1 with a seed-specific promotere0
- Gene
- BnWRI1e0
- Outcome
- oil accumulatione0
- Direction
- stabilizede0
- Qualifier
- heat-stress conditione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BnWRI1 with a seed-specific promoter stabilized both oil accumulation and photosynthesis under the heat-stress condition”
e1“Brassica napus seed filling”
Claim · model-written summary
Seed-specific BnWRI1 overexpression stabilized photosynthesis under heat stress.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- Overexpression of BnWRI1 with a seed-specific promotere0
- Gene
- BnWRI1e0
- Outcome
- photosynthesise0
- Direction
- stabilizede0
- Qualifier
- heat-stress conditione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BnWRI1 with a seed-specific promoter stabilized both oil accumulation and photosynthesis under the heat-stress condition”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress inhibited several sugar transporter genes.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- heat stresse0
- Gene
- sugar transporter genese0
- Outcome
- sugar transporter genese0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A number of sugar transporter genes were inhibited by heat stress”
e1“Brassica napus seed filling”
Claim · model-written summary
Heat stress impaired carbohydrate incorporation into triacylglycerols.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- heat stresse2
- Outcome
- incorporation of carbohydrates into triacylglycerols was impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“incorporation of carbohydrates into triacylglycerols was impaired”
e1“Brassica napus seed filling”
e2“heat stress resulted in reduction of seed oil accumulation”
Claim · model-written summary
The authors propose that photosynthetic disturbance, impaired carbohydrate incorporation and BnWRI1 deregulation may cause reduced seed oil accumulation.
- Taxon
- Brassica napusBrassica napuse1
- Gene
- BnWRI1e0
- Outcome
- decreased oil accumulation in the seede0
- Qualifier
- disturbances of the seed photosynthesis machinery, impairment of carbohydrates incorporation into triacylglycerols and transcriptional deregulation of the BnWRI1 pathway by heat stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disturbances of the seed photosynthesis machinery, impairment of carbohydrates incorporation into triacylglycerols and transcriptional deregulation of the BnWRI1 pathway by heat stress might be the major cause of decreased oil accumulation in the seed”
e1“Brassica napus seed filling”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.