Paper · 2019 · AIP conference proceedings

Agrobacterial cytokinin synthesis gene ipt increases plant resistance to high doses of copper ions

A. A. Ermoshin; I. S. Kiseleva; S. A. Shatunova

4
claims
8
verbatim quotes
2
citations
1
research areas
—
Not open access

Abstract

Plant lines expressing an agrobacterial cytokinin synthesis gene ipt were obtained by agrobacterial transformation of intact tobacco seeds. Transgenic lines were grown on MS medium with 100 μM and 200 μM of copper ions, while control lines died under such conditions. Determination of biochemical stress markers showed that copper ions cause a 2-fold increase in membrane lipid peroxidation and a higher activity of superoxide dismutase in control plants, while in transgenic lines, these indicators were significantly less. The role of ipt gene is discussing.

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Transgenic ipt lines grown on MS medium with 100 μM and 200 μM copper ions survived, whereas control lines died.

Material
Transgenic linese1
Treatment
100 μM and 200 μM of copper ionse1
Gene
ipte0
Outcome
diede1
Comparator
control linese1
Setting
MS mediume1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Plant lines expressing an agrobacterial cytokinin synthesis gene ipt were obtained by agrobacterial transformation of intact tobacco seeds”

✓ located verbatim in the source · abstract, characters 0–138 · show in text ↑
e1

“Transgenic lines were grown on MS medium with 100 μM and 200 μM of copper ions, while control lines died under such conditions”

✓ located verbatim in the source · abstract, characters 140–266 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper ions raised membrane lipid peroxidation 2-fold in control plants.

Treatment
copper ionse2
Outcome
membrane lipid peroxidatione2
Direction
increasee2
Value
2-folde2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Plant lines expressing an agrobacterial cytokinin synthesis gene ipt were obtained by agrobacterial transformation of intact tobacco seeds”

✓ located verbatim in the source · abstract, characters 0–138 · show in text ↑
e2

“Determination of biochemical stress markers showed that copper ions cause a 2-fold increase in membrane lipid peroxidation and a higher activity of superoxide dismutase in control plants, while in transgenic lines, these indicators were significantly less”

✓ located verbatim in the source · abstract, characters 268–523 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper ions raised superoxide dismutase activity in control plants.

Treatment
copper ionse2
Outcome
activity of superoxide dismutasee2
Direction
highere2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Plant lines expressing an agrobacterial cytokinin synthesis gene ipt were obtained by agrobacterial transformation of intact tobacco seeds”

✓ located verbatim in the source · abstract, characters 0–138 · show in text ↑
e2

“Determination of biochemical stress markers showed that copper ions cause a 2-fold increase in membrane lipid peroxidation and a higher activity of superoxide dismutase in control plants, while in transgenic lines, these indicators were significantly less”

✓ located verbatim in the source · abstract, characters 268–523 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Transgenic lines under copper ions had significantly lower stress markers than control plants.

Material
transgenic linese2
Treatment
copper ionse2
Outcome
these indicatorse2
Direction
significantly lesse2
Comparator
control plantse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Plant lines expressing an agrobacterial cytokinin synthesis gene ipt were obtained by agrobacterial transformation of intact tobacco seeds”

✓ located verbatim in the source · abstract, characters 0–138 · show in text ↑
e2

“Determination of biochemical stress markers showed that copper ions cause a 2-fold increase in membrane lipid peroxidation and a higher activity of superoxide dismutase in control plants, while in transgenic lines, these indicators were significantly less”

✓ located verbatim in the source · abstract, characters 268–523 · show in text ↑

copper atom · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative
See all 4 papers →

copper atom · physiology: oxidative · Nicotiana tabacum (tobacco)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.