Abstract
An ultra high performance liquid chromatography with triple quadrupole mass spectrometry method for the determination of free and bound phenolic acids in tobacco plant and soil was developed. A simple solid-phase extraction, which used Polar Enhanced Polymer column as stationary phase and methanol as mobile phase, was used for the clean-up of bound phenolic acids, and a liquid-phase extraction using chloroform as solvent was used to purify free phenolic acids. With our method, 18 phenolic acids in rhizosphere soil of continuous cropping flue-cured cultivar k326 were separated and determined within 6 min with recoveries of 82-107% and relative standard deviations (n = 5) of 1.1-4.8%. Results showed that free phenolic acids accounted for 0-9, 92-100, and 69-100% of total phenolic acids in rhizosphere soil, cultivar k326 roots and leaves, respectively. Results also revealed that p-hydroxybenzoic acid, p-coumaric acid, vanillic acid, ferulic acid, and syringic acid were the predominant phenolic acids in rhizosphere soil of cultivar k326, and continuous cropping of cultivar k326 in the same farmland could lead to the accumulation of these phenolic acids in soil except syringic acid. The determination of phenolic acids provided detailed information for evaluating their source and characteristics in continuous cropping tobacco plant and soil.
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Claims 7
Claim · model-written summary
The UHPLC-MS/MS method separated and determined 18 phenolic acids in rhizosphere soil of continuous-cropping tobacco cultivar k326 within 6 min with recoveries of 82-107%.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- cultivar k326e0
- Outcome
- recoveriese0
- Value
- 82-107%e0
- Qualifier
- within 6 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“18 phenolic acids in rhizosphere soil of continuous cropping flue-cured cultivar k326 were separated and determined within 6 min with recoveries of 82-107%”
t0“tobacco”
Claim · model-written summary
The method gave relative standard deviations (n = 5) of 1.1-4.8%.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- relative standard deviationse0
- Value
- 1.1-4.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative standard deviations (n = 5) of 1.1-4.8%”
t0“tobacco”
Claim · model-written summary
Free phenolic acids accounted for 0-9% of total phenolic acids in rhizosphere soil of tobacco cultivar k326.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultivar k326e0
- Organ / stage
- rhizosphere soile0
- Outcome
- free phenolic acidse0
- Value
- 0-9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free phenolic acids accounted for 0-9, 92-100, and 69-100% of total phenolic acids in rhizosphere soil, cultivar k326 roots and leaves, respectively”
t0“tobacco”
Claim · model-written summary
Free phenolic acids accounted for 92-100% of total phenolic acids in roots of tobacco cultivar k326.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultivar k326e0
- Organ / stage
- rootse0
- Outcome
- free phenolic acidse0
- Value
- 92-100e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free phenolic acids accounted for 0-9, 92-100, and 69-100% of total phenolic acids in rhizosphere soil, cultivar k326 roots and leaves, respectively”
t0“tobacco”
Claim · model-written summary
Free phenolic acids accounted for 69-100% of total phenolic acids in leaves of tobacco cultivar k326.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultivar k326e0
- Organ / stage
- leavese0
- Outcome
- free phenolic acidse0
- Value
- 69-100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free phenolic acids accounted for 0-9, 92-100, and 69-100% of total phenolic acids in rhizosphere soil, cultivar k326 roots and leaves, respectively”
t0“tobacco”
Claim · model-written summary
p-Hydroxybenzoic acid, p-coumaric acid, vanillic acid, ferulic acid and syringic acid were the predominant phenolic acids in rhizosphere soil of cultivar k326.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultivar k326e0
- Organ / stage
- rhizosphere soile0
- Outcome
- predominant phenolic acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“p-hydroxybenzoic acid, p-coumaric acid, vanillic acid, ferulic acid, and syringic acid were the predominant phenolic acids in rhizosphere soil of cultivar k326”
t0“tobacco”
Claim · model-written summary
Continuous cropping of cultivar k326 in the same farmland could lead to accumulation of these phenolic acids in soil, except syringic acid.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultivar k326e0
- Treatment
- continuous cropping of cultivar k326 in the same farmlande0
- Outcome
- accumulation of these phenolic acids in soile0
- Direction
- accumulatione0
- Qualifier
- except syringic acide0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“continuous cropping of cultivar k326 in the same farmland could lead to the accumulation of these phenolic acids in soil except syringic acid”
t0“tobacco”
The same questions in other papers
rotation · quality: phytochemicals · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.