Abstract
Horizontal transmission of virulence attenuating hypoviruses of Cryphonectria parasitica is restricted by an allorecognition system termed vegetative incompatibility (vic). A super donor formulation of two engineered C. parasitica strains (SD328/SD82) with gene disruptions at four of six vic loci transmitted hypovirus to strains in the laboratory independent of vic genotype. We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting. Hypovirulent (HV) isolates were recovered from 94% of cankers treated with the hypovirus-infected SD328/82 formulation compared to 51% of cankers treated with a hypovirus-infected EU5/6 formulation (strains having the same vic genotypes as SD strains but lacking vic gene disruptions). Overall, the SD328/82 formulation transmitted hypovirus into more divergent vic genotypes compared to the EU5/6 formulation. These results demonstrate the SD328/82 formulation can serve as an enhanced hypovirus vector for highly divergent C. parasitica populations.
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Claims 4
Claim · model-written summary
A super donor formulation of two engineered C. parasitica strains transmitted hypovirus to laboratory strains independent of vic genotype.
- Taxon
- American chestnut
Castanea dentatae0 - Treatment
- super donor formulation of two engineered C. parasitica strains (SD328/SD82)e1
- Interactor
- C. parasitica
Cryphonectria parasiticae0pathogen - Outcome
- transmitted hypoviruse1
- Qualifier
- independent of vic genotypee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting”
e1“A super donor formulation of two engineered C. parasitica strains (SD328/SD82) with gene disruptions at four of six vic loci transmitted hypovirus to strains in the laboratory independent of vic genotype”
Claim · model-written summary
The SD328/82 formulation transmitted hypovirus to a diverse natural C. parasitica population infecting American chestnut in a forest setting.
- Taxon
- American chestnutCastanea dentatae0
- Treatment
- SD328/82 formulatione0
- Interactor
- C. parasiticaCryphonectria parasiticae0pathogen
- Outcome
- transmission of hypoviruse0
- Setting
- forest settinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting”
Claim · model-written summary
Hypovirulent isolates were recovered from 94% of cankers treated with the SD328/82 formulation compared with 51% for the EU5/6 formulation.
- Taxon
- American chestnutCastanea dentatae0
- Treatment
- SD328/82 formulatione1
- Interactor
- C. parasiticaCryphonectria parasiticae0pathogen
- Outcome
- Hypovirulent (HV) isolates were recoverede1
- Direction
- higherinferrede1
- Value
- 94%e1
- Qualifier
- 51% of cankerse1
- Comparator
- EU5/6 formulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting”
e1“Hypovirulent (HV) isolates were recovered from 94% of cankers treated with the hypovirus-infected SD328/82 formulation compared to 51% of cankers treated with a hypovirus-infected EU5/6 formulation”
Claim · model-written summary
The SD328/82 formulation transmitted hypovirus into more divergent vic genotypes than the EU5/6 formulation.
- Taxon
- American chestnutCastanea dentatae0
- Treatment
- SD328/82 formulatione1
- Interactor
- C. parasiticaCryphonectria parasiticae0pathogen
- Outcome
- divergent vic genotypese1
- Direction
- moree1
- Comparator
- EU5/6 formulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We now report the transmission of hypovirus by the SD328/82 formulation to a diverse, natural C. parasitica population infecting American chestnut in a forest setting”
e1“Overall, the SD328/82 formulation transmitted hypovirus into more divergent vic genotypes compared to the EU5/6 formulation”