Abstract
The isolation of good quality DNA is the prerequisite for molecular research in any plant. However, the presence of high polyphenols, tannins and polysaccharides contents in leaf tissue of litchi leaf makes the genomic DNA extraction more difficult. In this study an efficient and sustainable method of genomic DNA extraction procedure is developed. This protocol is an improvement over the existing CTAB methods. Fully expanded, fresh, tender and purplish/reddish brown leaf was used to extract quality DNA. The addition of polyvinylpyrrolidone (PVP) and mercapto-ethanol was effective in the elimination of secondary metabolites, particularly polyphenolic compounds. The incorporation of high salt washing step was also efficient to remove polysaccharides. Therefore, results from electrophoresis, ratios of A260nm and A280nm and marker studies suggested that this modified method is more efficient to extract high-quality DNA compared to other reported conventional methods.
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Claims 3
Claim · model-written summary
The modified method extracted high-quality DNA from litchi leaf more efficiently than other reported conventional methods.
LitchiOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“genomic DNA from litchi”
e1“Fully expanded, fresh, tender and purplish/reddish brown leaf was used to extract quality DNA”
e2“suggested that this modified method is more efficient to extract high-quality DNA compared to other reported conventional methods”
Claim · model-written summary
Adding PVP and mercapto-ethanol was effective in eliminating secondary metabolites, particularly polyphenolic compounds, from litchi leaf DNA extracts.
- Treatment
- polyvinylpyrrolidone (PVP) and mercapto-ethanole3
- Compound
- polyphenolic compoundse3
- Outcome
- elimination of secondary metabolitese3
- Direction
- effectivee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“genomic DNA from litchi”
e3“The addition of polyvinylpyrrolidone (PVP) and mercapto-ethanol was effective in the elimination of secondary metabolites, particularly polyphenolic compounds”
Claim · model-written summary
A high salt washing step was efficient in removing polysaccharides from litchi leaf DNA extracts.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“genomic DNA from litchi”
e4“The incorporation of high salt washing step was also efficient to remove polysaccharides”
The same questions in other papers
processing · molecular: metabolites · LitchiPositive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.