Abstract
L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as to assure the maximum coverage of the metabolome of the samples under study within a single run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds. The suitability of each platform and polarity (negative and positive) to determine each family of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (-)) and sterols and tocopherols (MS (+)). Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols).
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Claims 8
Claim · model-written summary
Complementary LC and GC high-resolution MS methods identified about 150 compounds across olive samples.
Verbatim
e0“The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies.”
Claim · model-written summary
Identified olive metabolites included triterpenic acids and dialcohols, tocopherols, and sterols.
- Outcome
- triterpenic acids and dialcohols, tocopherols, sterolse0
Not reportedTaxon
Verbatim
e0“The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds.”
Claim · model-written summary
Identified olive metabolites included free fatty acids and several phenolic compound subtypes.
- Outcome
- free fatty acids, and several sub-types of phenolic compoundse0
Not reportedTaxon
Verbatim
e0“The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds.”
Claim · model-written summary
LC-ESI-MS (+) most efficiently ionized phenolic acids, secoiridoids, flavonoids and lignans.
Verbatim
e0“LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans”
Claim · model-written summary
LC-APCI-MS was appropriate for pentacyclic triterpenic acids under negative polarity.
Verbatim
e0“LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (-))”
Claim · model-written summary
LC-APCI-MS was appropriate for sterols and tocopherols under positive polarity.
Verbatim
e0“LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (-)) and sterols and tocopherols (MS (+))”
Claim · model-written summary
Olive fruit skin had the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids.
- Organ / stage
- fruit skine0
- Outcome
- relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acidse0
- Direction
- higheste0
Not reportedTaxon
Verbatim
e0“fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids”
Claim · model-written summary
Olive seed oil had high relative levels of acetoxypinoresinol and tocopherols.
- Organ / stage
- seed oile0
- Outcome
- relative levels of acetoxypinoresinol and tocopherolse0
- Direction
- highe0
Not reportedTaxon
Verbatim
e0“seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols”