Abstract
The content of grain protein (GPC) in cereals is an important part of total protein in human food. Exploring and utilizing new GPC genes is one of the most effective approaches for wheat quality breeding. Three homoeologues of TaAAP6(−3A , 3B , 3D) were cloned by homology cloning from OsAAP6 .Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat. Association analysis indicated that TaAAP6-3B-I is significantly correlated with higher GPC than that of TaAAP6-3B-II for 115 wheat lines in all five environments. TaAAP6-3B-I, the favored allele of TaAAP6-3B, was preferentially expressed in preliminary developing grain stage. Two functional markers were developed to discriminate 197F 2 populations and the result showed that TaAAP6-3B-I (high-protein content) was completely dominant. Two cis -regulatory elements appear to be associated with high GPC were found in the 5’UTR of TaAAP6-3B-I .The change of the TaAAP6-3B locus types indicated that the gene was subjected to selection pressures during long process of artificial selection. TaAAP6-3B is a regulator of GPC and its favored allele TaAAP6-3B-I exhibits an obvious potential application in wheat high-GPC breeding.
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Claims 9
Claim · model-written summary
TaAAP6 homoeologues were preferentially expressed in developing wheat grains.
- Taxon
- wheat
Triticum aestivume1 - Organ / stage
- developing grainse1
- Gene
- TaAAP6 homoeologuese1
- Outcome
- preferentially expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat”
Claim · model-written summary
TaAAP6-3B-I is significantly correlated with higher grain protein content than TaAAP6-3B-II in 115 wheat lines across five environments.
- Taxon
- wheatTriticum aestivume2
- Material
- 115 wheat linese2
- Gene
- TaAAP6-3B-Ie2
- Outcome
- GPCe2
- Direction
- highere2
- Comparator
- TaAAP6-3B-IIe2
- Setting
- all five environmentse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Association analysis indicated that TaAAP6-3B-I is significantly correlated with higher GPC than that of TaAAP6-3B-II for 115 wheat lines in all five environments”
Claim · model-written summary
TaAAP6-3B-I was preferentially expressed in the preliminary developing grain stage of wheat.
- Taxon
- wheatTriticum aestivume1
- Organ / stage
- preliminary developing grain stagee3
- Gene
- TaAAP6-3B-Ie3
- Outcome
- preferentially expressede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat”
e3“TaAAP6-3B-I, the favored allele of TaAAP6-3B, was preferentially expressed in preliminary developing grain stage”
Claim · model-written summary
The high-protein TaAAP6-3B-I allele was completely dominant in 197F2 populations.
- Taxon
- wheatTriticum aestivume1
- Material
- 197F 2 populationse4
- Gene
- TaAAP6-3B-Ie4
- Outcome
- high-protein contente4
- Direction
- completely dominante4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat”
e4“Two functional markers were developed to discriminate 197F 2 populations and the result showed that TaAAP6-3B-I (high-protein content) was completely dominant”
Claim · model-written summary
Two functional markers were developed to discriminate 197F2 populations for TaAAP6-3B-I.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaAAP6-3B-Ie4
- Outcome
- functional markerse4
- Value
- Twoe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat”
e4“Two functional markers were developed to discriminate 197F 2 populations and the result showed that TaAAP6-3B-I (high-protein content) was completely dominant”
Claim · model-written summary
Two cis-regulatory elements in the 5’UTR of TaAAP6-3B-I appear to be associated with high GPC in wheat.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaAAP6-3B-Ie5
- Outcome
- high GPCe5
- Qualifier
- Two cis -regulatory elementse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat”
e5“Two cis -regulatory elements appear to be associated with high GPC were found in the 5’UTR of TaAAP6-3B-I”
Claim · model-written summary
The change of TaAAP6-3B locus types indicates selection pressure on the gene during artificial selection of wheat.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaAAP6-3Be6
- Outcome
- selection pressurese6
- Qualifier
- during long process of artificial selectione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Temporal and spatial expression analysis showed that TaAAP6 homoeologues were preferentially expressed in developing grains, and TaAAP6-3B may play a major role in regulating GPC in wheat”
e6“The change of the TaAAP6-3B locus types indicated that the gene was subjected to selection pressures during long process of artificial selection”
Claim · model-written summary
TaAAP6-3B is a regulator of grain protein content in wheat.
- Taxon
- wheatTriticum aestivume7
- Gene
- TaAAP6-3Be7
- Outcome
- regulator of GPCe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“TaAAP6-3B is a regulator of GPC and its favored allele TaAAP6-3B-I exhibits an obvious potential application in wheat high-GPC breeding”
Claim · model-written summary
The favoured TaAAP6-3B-I allele has potential application in wheat high-GPC breeding.
- Taxon
- wheatTriticum aestivume7
- Gene
- TaAAP6-3B-Ie7
- Outcome
- potential application in wheat high-GPC breedinge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“TaAAP6-3B is a regulator of GPC and its favored allele TaAAP6-3B-I exhibits an obvious potential application in wheat high-GPC breeding”