Abstract
Accumulation of storage compounds during seed development plays an important role in the life cycle of oilseed plants; these compounds provide carbon and energy resources to support the establishment of seedlings. In this study, we show that BnCIPK9 has a broad expression pattern in Brassica napus L. tissues and that wounding stress strongly induces its expression. The overexpression of BnCIPK9 during seed development reduced oil synthesis in transgenic B. napus compared to that observed in wild-type (WT) plants. Functional analysis revealed that seed oil content (OC) of complementation lines was similar to that of WT plants, whereas OC in Arabidopsis thaliana (L.) Heynh. Atcipk9 knockout mutants ( cipk9 ) was higher than that of WT plants. Seedling of cipk9 mutants failed to establish roots on a sugar-free medium, but root establishment could be rescued by supplementation of sucrose or glucose. The phenotype of complementation transgenic lines was similar to that of WT plants when grown on sugar-free medium. Mutants, cipk9 , cbl2 , and cbl3 presented similar phenotypes, suggesting that CIPK9, CBL2, and CBL3 might work together and play similar roles in root establishment under sugar-free condition. This study showed that BnCIPK9 and AtCIPK9 encode a protein kinase that is involved in sugar-related response and plays important roles in the regulation of energy reserves. Our results suggest that AtCIPK9 negatively regulates lipid accumulation and has a significant effect on early seedling establishment in A. thaliana . The functional characterization of CIPK9 provides insights into the regulation of OC, and might be used for improving OC in B. napus . We believe that our study makes a significant contribution to the literature because it provides information on how CIPKs coordinate stress regulation and energy signaling.
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Claims 11
Claim · model-written summary
BnCIPK9 was broadly expressed in Brassica tissues.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Organ / stage
- tissuese0
- Gene
- BnCIPK9e0
- Outcome
- broad expression patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“BnCIPK9 has a broad expression pattern in Brassica napus L. tissues”
t0“Brassica napus L.”
Claim · model-written summary
Wounding stress strongly induced BnCIPK9 expression.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- wounding stresse0
- Outcome
- expressione0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounding stress strongly induces its expression.”
t0“Brassica napus L.”
Claim · model-written summary
BnCIPK9 overexpression reduced oil synthesis in transgenic B. napus compared with wild type.
- Taxon
- Brassica napus L.Brassica napust0
- Organ / stage
- seed developmente0
- Treatment
- overexpression of BnCIPK9e0
- Gene
- BnCIPK9e0
- Outcome
- oil synthesise0
- Direction
- reducede0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of BnCIPK9 during seed development reduced oil synthesis in transgenic B. napus compared to that observed in wild-type (WT) plants.”
t0“Brassica napus L.”
Claim · model-written summary
Seed oil content in complementation lines was similar to wild type.
- Taxon
- Arabidopsis thaliana (L.) Heynh.
Arabidopsis thalianat0 - Treatment
- complementation linese0
- Outcome
- seed oil content (OC)e0
- Direction
- similare0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed oil content (OC) of complementation lines was similar to that of WT plants”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Arabidopsis Atcipk9 knockout mutants had higher oil content than wild type.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Material
- cipk9e0
- Treatment
- Atcipk9 knockout mutantse0
- Gene
- Atcipk9e0
- Outcome
- OCe0
- Direction
- highere0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OC in Arabidopsis thaliana (L.) Heynh. Atcipk9 knockout mutants ( cipk9 ) was higher than that of WT plants.”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
cipk9 seedlings failed to establish roots on sugar-free medium.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Material
- cipk9 mutantse0
- Organ / stage
- Seedlinge0
- Outcome
- failed to establish rootse0
- Setting
- sugar-free mediume0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedling of cipk9 mutants failed to establish roots on a sugar-free medium”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Sucrose supplementation rescued root establishment in cipk9 seedlings.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Material
- cipk9 mutantse0
- Treatment
- supplementation of sucrosee0
- Outcome
- root establishmente0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedling of cipk9 mutants failed to establish roots on a sugar-free medium, but root establishment could be rescued by supplementation of sucrose or glucose.”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Glucose supplementation rescued root establishment in cipk9 seedlings.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Material
- cipk9 mutantse0
- Treatment
- glucosee0
- Outcome
- root establishmente0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedling of cipk9 mutants failed to establish roots on a sugar-free medium, but root establishment could be rescued by supplementation of sucrose or glucose.”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
Complementation lines resembled wild type on sugar-free medium.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Material
- complementation transgenic linese0
- Outcome
- phenotypee0
- Comparator
- WT plantse0
- Setting
- sugar-free mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phenotype of complementation transgenic lines was similar to that of WT plants when grown on sugar-free medium.”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
cipk9, cbl2 and cbl3 mutants had similar phenotypes under sugar-free conditions.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Material
- cipk9 , cbl2 , and cbl3e0
- Outcome
- similar phenotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutants, cipk9 , cbl2 , and cbl3 presented similar phenotypes”
t0“Arabidopsis thaliana (L.) Heynh.”
Claim · model-written summary
The authors infer that CIPK9, CBL2 and CBL3 may jointly support root establishment without sugar.
- Taxon
- Arabidopsis thaliana (L.) Heynh.Arabidopsis thalianat0
- Gene
- CIPK9, CBL2, and CBL3e0
- Outcome
- root establishmente0
- Qualifier
- under sugar-free conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that CIPK9, CBL2, and CBL3 might work together and play similar roles in root establishment under sugar-free condition.”
t0“Arabidopsis thaliana (L.) Heynh.”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.