Abstract
RNA silencing is a common mechanism that plays a key role in antiviral defense. To overcome host defense responses, plant viruses encode silencing-suppressor proteins to target one or several key steps in the silencing machinery. Here, we report that the P6 protein encoded by Strawberry vein banding virus (SVBV) is an RNA silencing suppressor through Agrobacterium-mediated co-infiltration assays. SVBV P6 protein can suppress green fluorescent protein (GFP) gene silencing induced by single-stranded RNA but not by double-stranded RNA. The P6 protein can also inhibit systemic silencing of GFP through interfering the systemic spread of GFP silencing signal. Subcellular localization study indicated that P6 protein formed irregular bodies and distributed in both cytoplasm and nucleus of Nicotiana benthamiana cells. Furthermore, deletion analysis indicated that a nuclear localization signal (NLS, aa 402-426) in the P6 protein is responsible for the silencing suppression efficiency. In addition, expression of the P6 protein via a Potato virus X (PVX)-based vectors induced more severe mosaic symptoms in N. benthamiana leaves, and transgenic N. benthamiana plants expressing P6 showed obvious vein yellowing as well as severe mosaic symptoms in leaves. Taken together, our results demonstrates that SVBV P6 is a suppressor of RNA silencing, possibly acting at a upstream step for dsRNA generation.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
SVBV P6 suppressed silencing induced by single-stranded RNA but not double-stranded RNA.
- Taxon
- Strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- single-stranded RNAe0
- Interactor
- SVBV
Strawberry vein banding viruse0pathogen - Gene
- SVBV P6 proteine0
- Outcome
- green fluorescent protein (GFP) gene silencinge0
- Direction
- suppresse0
- Comparator
- double-stranded RNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“SVBV P6 protein can suppress green fluorescent protein (GFP) gene silencing induced by single-stranded RNA but not by double-stranded RNA.”
t0“Strawberry”
Claim · model-written summary
P6 inhibited systemic GFP silencing by interfering with signal spread.
- Taxon
- StrawberryFragaria ×ananassat0
- Interactor
- Strawberry vein banding virusStrawberry vein banding virust0pathogen
- Gene
- P6 proteine0
- Outcome
- systemic silencing of GFPe0
- Direction
- inhibite0
- Qualifier
- interfering the systemic spread of GFP silencing signale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The P6 protein can also inhibit systemic silencing of GFP through interfering the systemic spread of GFP silencing signal.”
t0“Strawberry vein banding virus”
Claim · model-written summary
P6 formed irregular bodies in cytoplasm and nucleus of Nicotiana benthamiana cells.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- cytoplasm and nucleuse0
- Gene
- P6 proteine0
- Outcome
- irregular bodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P6 protein formed irregular bodies and distributed in both cytoplasm and nucleus of Nicotiana benthamiana cells.”
Claim · model-written summary
The P6 nuclear localization signal was responsible for suppression efficiency.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- P6 proteine0
- Outcome
- silencing suppression efficiencye0
- Qualifier
- nuclear localization signal (NLS, aa 402-426)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a nuclear localization signal (NLS, aa 402-426) in the P6 protein is responsible for the silencing suppression efficiency.”
t0“Strawberry”
Claim · model-written summary
P6 expression through PVX vectors induced more severe mosaic symptoms in N. benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- expression of the P6 protein via a Potato virus X (PVX)-based vectorse0
- Interactor
- Potato virus X (PVX)Potato virus Xe0pathogen
- Outcome
- mosaic symptomse0
- Direction
- more severee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the P6 protein via a Potato virus X (PVX)-based vectors induced more severe mosaic symptoms in N. benthamiana leaves”
Claim · model-written summary
Transgenic N. benthamiana expressing P6 showed vein yellowing and severe leaf mosaic.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Material
- transgenic N. benthamiana plants expressing P6e0
- Organ / stage
- leavese0
- Outcome
- obvious vein yellowing as well as severe mosaic symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic N. benthamiana plants expressing P6 showed obvious vein yellowing as well as severe mosaic symptoms in leaves.”
Claim · model-written summary
The authors propose that SVBV P6 acts upstream of dsRNA generation in silencing suppression.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- SVBV P6e0
- Outcome
- suppressor of RNA silencinge0
- Qualifier
- possibly acting at a upstream step for dsRNA generatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SVBV P6 is a suppressor of RNA silencing, possibly acting at a upstream step for dsRNA generation.”
t0“Strawberry”
The same questions in other papers
Solanum tuberosum · Potato virus X · Nicotiana benthamianaPositive and negative results
22 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
21 papers more in this group
See all 25 papers →transgenic · Potato virus X · Nicotiana benthamianaPositive and negative results
11 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.