Abstract
Premise of the Study We describe a highly efficient in vitro Lotus japonicus hairy root transformation system that is useful for the investigation of mycorrhizal symbiosis. Methods and Results The Agrobacterium rhizogenes –mediated transformation method was improved based on the binary plasmid ( pCAMBIA 1304) harboring green fluorescent protein and β‐glucuronidase genes for rapid detection. Transgenic hairy roots were grown within 13 days. These in vitro–cultured hairy roots can be inoculated with Rhizophagus irregularis , thus facilitating the investigation of the symbiosis between L. japonicus and arbuscular mycorrhizal fungi. Conclusions Compared with existing techniques, our protocol provides a simple and efficient A. rhizogenes– mediated transformation system for L. japonicus . The rapid induction of hairy roots can shorten the experimental time by at least one week.
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Claims 4
Claim · model-written summary
Transgenic Lotus japonicus hairy roots grew within 13 days.
- Taxon
- Lotus japonicus
Lotus japonicust0 - Outcome
- Transgenic hairy roots were growne0
- Value
- 13 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic hairy roots were grown within 13 days”
t0“Lotus japonicus”
Claim · model-written summary
Cultured Lotus japonicus hairy roots could be inoculated with Rhizophagus irregularis.
- Taxon
- Lotus japonicusLotus japonicust0
- Treatment
- inoculated with Rhizophagus irregularise0
- Interactor
- Rhizophagus irregularis
Rhizophagus irregularise0symbiont - Outcome
- can be inoculated with Rhizophagus irregularise0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These in vitro–cultured hairy roots can be inoculated with Rhizophagus irregularis”
t0“Lotus japonicus”
Claim · model-written summary
Rapid hairy root induction shortens experimental time by at least one week.
- Taxon
- Lotus japonicusLotus japonicust0
- Outcome
- experimental timee0
- Direction
- shortene0
- Value
- at least one weeke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rapid induction of hairy roots can shorten the experimental time by at least one week”
t0“Lotus japonicus”
Claim · model-written summary
The protocol is described as simple and efficient relative to existing techniques.
- Taxon
- L. japonicusLotus japonicuse0
- Outcome
- simple and efficient A. rhizogenes– mediated transformation systeme0
- Comparator
- existing techniquese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with existing techniques, our protocol provides a simple and efficient A. rhizogenes– mediated transformation system for L. japonicus”