Abstract
The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase (ssi2) has been proved to enhance pathogen resistance in several plants, while it's potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned TaSSI2 gene in wheat and provided several evidences of its involvement in multiple biological functions. By using barley stripe mosaic virus (BSMV)-induced gene silencing (VIGS) in wheat, it was found that TaSSI2 negatively regulated both powdery mildew and Fusarium head blight (FHB) resistance, which was consistent with the phenotype observed in knock-out mutants of Kronos. The expression of TaSSI2 was down-regulated by in vitro treatments of methyl jasmonate (MeJA), but positively regulated by salicylic acid (SA) and abscisic acid (ABA), implying the cross-talk between different hormone signaling pathways involved in wheat to regulate biotic stresses is still to be elucidated. Furthermore, the up-regulated expression of PR4 and PR5 indicated that TaSSI2 probably regulated FHB resistance by depressing the SA signaling pathway in wheat. In addition, the over-expression of TaSSI2 increased the content of linolenic acid (18:3) and subsequently enhanced drought tolerance of transgenic Brachypodium. This phenomenon might be associated with its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles. As a stearoyl-acyl carrier protein fatty acid desaturase, TaSSI2 was proposed to be involved in cell lipid metabolism and carried targets out of the cell from membrane or wax synthesis, resulting in enhanced drought tolerance in plant.
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Claims 10
Claim · model-written summary
TaSSI2 was cloned in wheat.
- Taxon
- wheat
Triticum aestivume0 - Gene
- TaSSI2e0
- Outcome
- cloned TaSSI2 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we cloned TaSSI2 gene in wheat”
Claim · model-written summary
TaSSI2 negatively regulated powdery mildew resistance.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaSSI2e0
- Outcome
- powdery mildewe0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSSI2 negatively regulated both powdery mildew and Fusarium head blight (FHB) resistance”
e1“biotic and abiotic stresses in wheat”
Claim · model-written summary
TaSSI2 negatively regulated Fusarium head blight resistance.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaSSI2e0
- Outcome
- Fusarium head blight (FHB) resistancee0
- Direction
- negativelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaSSI2 negatively regulated both powdery mildew and Fusarium head blight (FHB) resistance”
e1“biotic and abiotic stresses in wheat”
Claim · model-written summary
Methyl jasmonate down-regulated TaSSI2 expression.
- Taxon
- wheatTriticum aestivume1
- Treatment
- methyl jasmonate (MeJA)e0
- Gene
- TaSSI2e0
- Outcome
- expression of TaSSI2e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of TaSSI2 was down-regulated by in vitro treatments of methyl jasmonate (MeJA)”
e1“biotic and abiotic stresses in wheat”
Claim · model-written summary
Salicylic acid positively regulated TaSSI2 expression.
- Taxon
- wheatTriticum aestivume2
- Treatment
- salicylic acid (SA)e0
- Gene
- TaSSI2e1
- Outcome
- expression of TaSSI2e1
- Direction
- positively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“positively regulated by salicylic acid (SA)”
e1“The expression of TaSSI2”
e2“biotic and abiotic stresses in wheat”
Claim · model-written summary
Abscisic acid positively regulated TaSSI2 expression.
- Taxon
- wheatTriticum aestivume2
- Treatment
- abscisic acid (ABA)e0
- Gene
- TaSSI2e1
- Outcome
- expression of TaSSI2e1
- Direction
- positively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“positively regulated by salicylic acid (SA) and abscisic acid (ABA)”
e1“The expression of TaSSI2”
e2“biotic and abiotic stresses in wheat”
Claim · model-written summary
PR4 and PR5 expression was up-regulated, consistent with a proposed SA pathway role in FHB resistance.
- Taxon
- wheatTriticum aestivume0
- Gene
- PR4 and PR5e0
- Outcome
- up-regulated expression of PR4 and PR5e0
- Direction
- up-regulatede0
- Qualifier
- probably regulated FHB resistance by depressing the SA signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the up-regulated expression of PR4 and PR5 indicated that TaSSI2 probably regulated FHB resistance by depressing the SA signaling pathway in wheat”
Claim · model-written summary
TaSSI2 over-expression increased linolenic acid content in transgenic Brachypodium.
- Taxon
- Brachypodium
Brachypodiume0 - Treatment
- over-expression of TaSSI2e0
- Gene
- TaSSI2e0
- Outcome
- content of linolenic acid (18:3)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the over-expression of TaSSI2 increased the content of linolenic acid (18:3) and subsequently enhanced drought tolerance of transgenic Brachypodium”
Claim · model-written summary
TaSSI2 over-expression enhanced drought tolerance in transgenic Brachypodium.
- Taxon
- BrachypodiumBrachypodiume0
- Treatment
- over-expression of TaSSI2e0
- Gene
- TaSSI2e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the over-expression of TaSSI2 increased the content of linolenic acid (18:3) and subsequently enhanced drought tolerance of transgenic Brachypodium”
Claim · model-written summary
TaSSI2 localized throughout the cytosol and partly overlapped Golgi apparatus and secreted vesicles.
- Taxon
- BrachypodiumBrachypodiume1
- Gene
- TaSSI2e2
- Outcome
- subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesiclese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles”
e1“transgenic Brachypodium”
e2“TaSSI2 was proposed to be involved in cell lipid metabolism”
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33 papers in the same direction as this paper · 9 in the opposite direction
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See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.