Paper · 2018 · International Journal of Current Microbiology and Applied Sciences

Mutation-induced Polygenic Variability and Early Prediction of High Yielding Mutants in Greengram [Vigna radiata (L.) wilczek]

Tapas Ranjan Das; Bhabendra Baisakh

11
claims
24
verbatim quotes
2
citations
1
research areas
✓
Open access · cc-by-nc-sa

Abstract

Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52. In M2 generation, population mean got decrease with increase in variance for all the four traits studied and the magnitude of such changes varied with mutagens, their doses and the variety. Higher the dose of treatment greater the shift in mean and variance. The negative shift of mean was more pronounced in MH and its combined treatments in both the varieties. The M2 populations showed wider range of variations than the parent varieties. Genetic advance estimates showed that selection in many M2 treatment populations would be effective in bringing the improvement in yield/plant. Following selection among M2 plants and in M3 progenies on the basis of higher yield, ten mutant cultures in Sujata and eleven mutants in OBGG-52 were found significantly higher yield in M4 generations. Majority of them were from the groups of M2 mutagenic treatments sowing significantly higher population variance for yield/plants. Thus, selection of high yielding M2 plants in mutagenic treatments with significantly increased M2 variance for yield would be effective in isolation of high yielding micro mutant lines.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-sa. Read at the source ↗

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Claims 11

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Mutagen treatments decreased the population mean of greengram M2 populations for all four traits studied.

Treatment
mutagense2
Outcome
population meane1
Direction
decreasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e1

“In M2 generation, population mean got decrease with increase in variance for all the four traits studied”

✓ located verbatim in the source · abstract, characters 281–385 · show in text ↑
e2

“the magnitude of such changes varied with mutagens, their doses and the variety”

✓ located verbatim in the source · abstract, characters 390–469 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Mutagen treatments increased the population variance of greengram M2 populations for all four traits studied.

Treatment
mutagense2
Outcome
variancee1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e1

“In M2 generation, population mean got decrease with increase in variance for all the four traits studied”

✓ located verbatim in the source · abstract, characters 281–385 · show in text ↑
e2

“the magnitude of such changes varied with mutagens, their doses and the variety”

✓ located verbatim in the source · abstract, characters 390–469 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The negative shift of mean was more pronounced under MH and its combined treatments than under the other treatments in both greengram varieties.

Treatment
MH and its combined treatmentse3
Outcome
negative shift of meane3
Direction
more pronouncede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e3

“The negative shift of mean was more pronounced in MH and its combined treatments in both the varieties”

✓ located verbatim in the source · abstract, characters 540–642 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The M2 populations of greengram showed a wider range of variation than the parent varieties.

Outcome
range of variationse4
Direction
widere4
Comparator
parent varietiese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e4

“The M2 populations showed wider range of variations than the parent varieties”

✓ located verbatim in the source · abstract, characters 644–721 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Genetic advance estimates indicate that selection in many greengram M2 treatment populations would be effective for improving yield per plant.

Treatment
selection in many M2 treatment populationse5
Outcome
improvement in yield/plante5
Qualifier
would be effectivee5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e5

“Genetic advance estimates showed that selection in many M2 treatment populations would be effective in bringing the improvement in yield/plant”

✓ located verbatim in the source · abstract, characters 723–865 · show in text ↑
Observationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Ten greengram mutant cultures in Sujata had significantly higher yield in M4 generations.

Material
Sujatae6
Outcome
yielde6
Direction
highere6
Value
ten mutant culturese6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e6

“ten mutant cultures in Sujata and eleven mutants in OBGG-52 were found significantly higher yield in M4 generations”

✓ located verbatim in the source · abstract, characters 953–1068 · show in text ↑
Observationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Eleven greengram mutants in OBGG-52 had significantly higher yield in M4 generations.

Material
OBGG-52e6
Outcome
yielde6
Direction
highere6
Value
eleven mutantse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e6

“ten mutant cultures in Sujata and eleven mutants in OBGG-52 were found significantly higher yield in M4 generations”

✓ located verbatim in the source · abstract, characters 953–1068 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Most high-yielding mutants came from M2 mutagenic treatments with significantly higher population variance for yield per plant.

Treatment
M2 mutagenic treatmentse7
Outcome
population variance for yield/plantse7
Direction
highere7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e7

“Majority of them were from the groups of M2 mutagenic treatments sowing significantly higher population variance for yield/plants”

✓ located verbatim in the source · abstract, characters 1070–1199 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Selecting high-yielding M2 plants in mutagenic treatments with increased M2 variance for yield would be effective for isolating high-yielding micro mutant lines of greengram.

Treatment
selection of high yielding M2 plants in mutagenic treatmentse1
Outcome
isolation of high yielding micro mutant linese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e1

“selection of high yielding M2 plants in mutagenic treatments with significantly increased M2 variance for yield would be effective in isolation of high yielding micro mutant lines”

✓ located verbatim in the source · abstract, characters 1207–1386 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

A higher treatment dose produced a greater shift in mean and variance of greengram traits.

Treatment
dose of treatmente1
Outcome
shift in mean and variancee1
Direction
greatere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e1

“Higher the dose of treatment greater the shift in mean and variance”

✓ located verbatim in the source · abstract, characters 471–538 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The magnitude of trait changes in greengram M2 populations varied with mutagen, dose and variety.

Outcome
magnitude of such changese2
Qualifier
varied with mutagens, their doses and the varietye2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata var. radiata
Vigna radiata var. radiata · Taxon · picture of the speciesIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Three does each of gamma rays (200, 400 and 600Gy), ethyl methane sulphonate (0.2, 0.4 and 0.6%), nitrosoguanidine (0.005, 0.010, and 0.015%), maleic hydrazide (0.01, 0.02 and 0.03%) and in combination were administered to the seeds of two greengram varieties, Sujata and OBGG-52”

✓ located verbatim in the source · abstract, characters 0–279 · show in text ↑
e2

“the magnitude of such changes varied with mutagens, their doses and the variety”

✓ located verbatim in the source · abstract, characters 390–469 · show in text ↑