Abstract
SummaryThere are no reports on the possible protective role of trehalose in tropical monocot plants such as banana, when exposed to salt stress. In the present work, banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose [trehalose-6-phosphate synthase (tps); and trehalose-6-phosphate phosphatase (tpp)] under the control of the Arabidopsis rd29A drought-stress inducible promoter. Transformed (rd29A::tps-tpp; TF) and wild-type (WT) plants were subjected to three different concentrations of NaCl (100, 200, or 300 mM) for 9 d. The trehalose concentration in the leaves of WT plants was approx. 3.5 µg g−1 fresh weight (FW) when not exposed to salinity, and increased only slightly (to 4.0 µg g−1 FW) when exposed to 100 or 200 mM NaCl. In TF plants, however, the trehalose concentration was approx. 4.0 µg g−1 FW when not exposed to salinity, but increased with salinity, reaching values of ≥ 8.0 µg g−1 FW, when exposed to 300 mM NaCl. WT plants were severely affected when exposed to salinity, reducing their photosynthetic rates, (from 6.0 to 1.5 µmol CO2 m−2 s−1 at 0 and 200 mM NaCl, respectively, with an extensively damaged photosystem II [i.e., the ratio of maximum variable fluorescence to maximum fluorescence (Fv/Fm) decreased from 0.85 to 0.58, at 0 and 300 mM NaCl, respectively]. On the other hand, TF plants were able to withstand salt stress and showed a lower reduction in photosynthetic rate (from 7.0 to 4.0 µmol CO2 m−2 s−1, at 0 and 200 mM NaCl, respectively) and a lessaffected photosystem II (i.e., the Fv/Fm ratio only decreased from 0.85 to 0.75, at 0 and 300 mM NaCl, respectively). The superior ability of TF plants to protect their photosynthetic performance when exposed to salinity was confirmed by measuring additional chlorophyll fluorescence (CF) parameters. These results provide the first evidence that trehalose can protect against abiotic stress in a tropical monocot plant such as banana.
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Claims 8
Claim · model-written summary
Trehalose concentration in transformed banana plants increased with salinity, reaching at least 8.0 µg g−1 FW at 300 mM NaCl.
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e1“In TF plants, however, the trehalose concentration was approx. 4.0 µg g−1 FW when not exposed to salinity, but increased with salinity, reaching values of ≥ 8.0 µg g−1 FW, when exposed to 300 mM NaCl”
Claim · model-written summary
Trehalose concentration in wild-type banana leaves increased only slightly with 100 or 200 mM NaCl.
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e2“The trehalose concentration in the leaves of WT plants was approx. 3.5 µg g−1 fresh weight (FW) when not exposed to salinity, and increased only slightly (to 4.0 µg g−1 FW) when exposed to 100 or 200 mM NaCl”
Claim · model-written summary
Photosynthetic rate of wild-type banana plants was reduced from 6.0 to 1.5 µmol CO2 m−2 s−1 under salinity at 0 and 200 mM NaCl.
- Taxon
- bananae0
- Material
- WT plantse3
- Treatment
- exposed to salinitye3
- Outcome
- photosynthetic ratese3
- Direction
- reducinge3
- Value
- from 6.0 to 1.5 µmol CO2 m−2 s−1e3
- Qualifier
- at 0 and 200 mM NaCl, respectivelye3
Not reportedComparator
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e3“WT plants were severely affected when exposed to salinity, reducing their photosynthetic rates, (from 6.0 to 1.5 µmol CO2 m−2 s−1 at 0 and 200 mM NaCl, respectively”
Claim · model-written summary
Fv/Fm of wild-type banana plants decreased from 0.85 to 0.58 under salinity at 0 and 300 mM NaCl, indicating damaged photosystem II.
- Taxon
- bananae0
- Treatment
- exposed to salinitye3
- Outcome
- Fv/Fme4
- Direction
- decreasede4
- Value
- from 0.85 to 0.58e4
- Qualifier
- at 0 and 300 mM NaCl, respectivelye4
Not reportedComparator
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e3“WT plants were severely affected when exposed to salinity, reducing their photosynthetic rates, (from 6.0 to 1.5 µmol CO2 m−2 s−1 at 0 and 200 mM NaCl, respectively”
e4“the ratio of maximum variable fluorescence to maximum fluorescence (Fv/Fm) decreased from 0.85 to 0.58, at 0 and 300 mM NaCl, respectively”
Claim · model-written summary
Transformed banana plants showed a lower reduction in photosynthetic rate under salt stress (from 7.0 to 4.0 µmol CO2 m−2 s−1 at 0 and 200 mM NaCl).
- Taxon
- bananae0
- Material
- TF plantse5
- Treatment
- salt stresse5
- Outcome
- reduction in photosynthetic ratee5
- Direction
- lowere5
- Value
- from 7.0 to 4.0 µmol CO2 m−2 s−1e5
- Qualifier
- at 0 and 200 mM NaCl, respectivelye5
Not reportedComparator
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e5“On the other hand, TF plants were able to withstand salt stress and showed a lower reduction in photosynthetic rate (from 7.0 to 4.0 µmol CO2 m−2 s−1, at 0 and 200 mM NaCl, respectively)”
Claim · model-written summary
Fv/Fm of transformed banana plants decreased only from 0.85 to 0.75 at 300 mM NaCl.
- Taxon
- bananae0
- Material
- TF plantse5
- Treatment
- 300 mM NaCle6
- Outcome
- Fv/Fm ratioe6
- Direction
- only decreasede6
- Value
- from 0.85 to 0.75e6
- Qualifier
- at 0 and 300 mM NaCl, respectivelye6
Not reportedComparator
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e5“On the other hand, TF plants were able to withstand salt stress and showed a lower reduction in photosynthetic rate (from 7.0 to 4.0 µmol CO2 m−2 s−1, at 0 and 200 mM NaCl, respectively)”
e6“the Fv/Fm ratio only decreased from 0.85 to 0.75, at 0 and 300 mM NaCl, respectively”
Claim · model-written summary
Transformed banana plants protected their photosynthetic performance better under salinity than wild-type plants.
- Taxon
- bananae0
- Material
- TF plantse7
- Treatment
- exposed to salinitye7
- Outcome
- photosynthetic performancee7
- Direction
- superiore7
- Qualifier
- chlorophyll fluorescence (CF) parameterse7
Not reportedComparator
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e7“The superior ability of TF plants to protect their photosynthetic performance when exposed to salinity was confirmed by measuring additional chlorophyll fluorescence (CF) parameters”
Claim · model-written summary
Trehalose can protect banana against abiotic stress.
Verbatim
e0“banana plants (Musa acuminata ‘Grand Nain’) were transformed with a fusion of two genes from Saccharomyces cerevisiae involved in the biosynthetic pathway of trehalose”
e1“These results provide the first evidence that trehalose can protect against abiotic stress in a tropical monocot plant such as banana”
The same questions in other papers
sodium chloride · molecular: metabolites · MusaPositive and negative results
1 in the opposite direction
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salinity · physiology: photosynthesis · MusaPositive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.