Paper · 2017 · Genetics and Molecular Research

Expressed sequence tags related to nitrogen metabolism in maize inoculated with Azospirillum brasilense

L. Pereira-Defilippi; E.M. Pereira; F.M. Silva; et al.

4
claims
13
verbatim quotes
24
citations
3
research areas
✓
Open access

Abstract

The relative quantitative real-time expression of two expressed sequence tags (ESTs) codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3) was performed for genotypes inoculated with Azospirillum brasilense. Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised under controlled greenhouse conditions, in six treatment groups corresponding to different forms of inoculation and different levels of nitrogen application by top-dressing. The genotypes presented distinct responses to inoculation with A. brasilense. Increases in the expression of ZmNR were observed for the hybrids, while V4 only displayed a greater level of expression when the plants received nitrogenous fertilization by top-dressing and there was no inoculation. The expression of the ZmGln1-3EST was induced by A. brasilense in the hybrids and the variety V4. In contrast, the variety V2 did not respond to inoculation.

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Claims 4

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Azospirillum brasilense inoculation increased ZmNR expression in the commercial hybrids AG7098 and 2B707 in the greenhouse.

Taxon
maizeZea mayse0
Material
AG7098 and 2B707e1
Treatment
inoculated with Azospirillum brasilenset0
Interactor
Azospirillum brasilenseAzospirillum brasilenset0symbiont
Gene
ZmNRe0
Outcome
expression of ZmNRe2
Direction
Increasese2
Setting
controlled greenhouse conditionse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3)”

✓ located verbatim in the source · abstract, characters 85–205 · show in text ↑
e1

“Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised under controlled greenhouse conditions”

✓ located verbatim in the source · abstract, characters 275–433 · show in text ↑
e2

“Increases in the expression of ZmNR were observed for the hybrids”

✓ located verbatim in the source · abstract, characters 647–712 · show in text ↑
t0

“maize inoculated with Azospirillum brasilense”

✓ located verbatim in the source · title, characters 58–103 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Synthetic variety V4 showed greater ZmNR expression with nitrogen top-dressing and no inoculation.

Taxon
maizeZea mayse0
Material
V4e1
Treatment
nitrogenous fertilization by top-dressinge3
Gene
ZmNRe0
Outcome
level of expressione3
Direction
greatere3
Comparator
no inoculatione3
Setting
controlled greenhouse conditionse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3)”

✓ located verbatim in the source · abstract, characters 85–205 · show in text ↑
e1

“Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised under controlled greenhouse conditions”

✓ located verbatim in the source · abstract, characters 275–433 · show in text ↑
e3

“while V4 only displayed a greater level of expression when the plants received nitrogenous fertilization by top-dressing and there was no inoculation”

✓ located verbatim in the source · abstract, characters 714–863 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Azospirillum brasilense induced ZmGln1-3 expression in the hybrids and in variety V4.

Taxon
maizeZea mayse0
Material
the hybrids and the variety V4e4
Treatment
A. brasilensee4
Interactor
Azospirillum brasilenseAzospirillum brasilenset0symbiont
Gene
ZmGln1-3e0
Outcome
expression of the ZmGln1-3ESTe4
Direction
inducede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3)”

✓ located verbatim in the source · abstract, characters 85–205 · show in text ↑
e4

“The expression of the ZmGln1-3EST was induced by A. brasilense in the hybrids and the variety V4”

✓ located verbatim in the source · abstract, characters 865–961 · show in text ↑
t0

“maize inoculated with Azospirillum brasilense”

✓ located verbatim in the source · title, characters 58–103 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Variety V2 did not respond to Azospirillum brasilense inoculation in ZmGln1-3 expression.

Taxon
maizeZea mayst0
Material
V2e1
Treatment
inoculatione5
Interactor
Azospirillum brasilenseAzospirillum brasilenset0symbiont
Outcome
responde5
Direction
did not responde5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“Two commercial single-cross hybrids (AG7098 and 2B707) and two experimental synthetic varieties (V2 and V4) were raised under controlled greenhouse conditions”

✓ located verbatim in the source · abstract, characters 275–433 · show in text ↑
e5

“In contrast, the variety V2 did not respond to inoculation”

✓ located verbatim in the source · abstract, characters 963–1021 · show in text ↑
t0

“maize inoculated with Azospirillum brasilense”

✓ located verbatim in the source · title, characters 58–103 · show in text ↑

inoculation · Azospirillum brasilense · Zea mays (maize)Results differ

39 papers: 30 positive, 9 negative or no effect

8 papers in the same direction as this paper · 30 in the opposite direction

  1. no effect
  2. positive
  3. positive

35 papers more in this group

See all 39 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.