Abstract
Chitinases belong to a conserved protein family and play multiple roles in defense, development and growth regulation in plants. Here, we identified a secreted chitinase‐like protein, OsCLP , which functions in rice growth. A T‐ DNA insertion mutant of OsCLP ( osclp ) showed significant retardation of root and shoot growth. A comparative proteomic analysis was carried out using root tissue of wild‐type and the osclp mutant to understand the OsCLP ‐mediated rice growth retardation. Results obtained revealed that proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1 ) were differentially regulated in osclp roots. Fura‐2 molecular probe staining, which is an intracellular calcium indicator, and inductively coupled plasma‐mass spectrometry ( ICP‐MS ) analysis suggested that the intracellular calcium content was significantly lower in roots of osclp as compared with the wild‐type. Exogenous application of Ca 2+ resulted in successful recovery of both primary and lateral root growth in osclp . Moreover, overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected. Taken together, our results highlight the involvement of OsCLP in rice growth by regulating the intracellular calcium concentrations.
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Claims 9
Claim · model-written summary
The osclp insertion mutant had significantly retarded root and shoot growth.
- Taxon
- rice
Oryza sativae1 - Material
- osclpe0
- Gene
- OsCLPe0
- Outcome
- root and shoot growthe0
- Direction
- retardatione0
- Qualifier
- T‐ DNA insertion mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A T‐ DNA insertion mutant of OsCLP ( osclp ) showed significant retardation of root and shoot growth.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Proteins related to glycolysis, stress adaptation and calcium signaling were differentially regulated in osclp roots.
- Taxon
- riceOryza sativae1
- Material
- osclpe0
- Organ / stage
- rootse0
- Outcome
- proteins related to glycolysise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1 ) were differentially regulated in osclp roots.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Intracellular calcium content was significantly lower in osclp roots than in wild type.
- Taxon
- riceOryza sativae1
- Material
- osclpe0
- Organ / stage
- rootse0
- Outcome
- intracellular calcium contente0
- Direction
- lowere0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“intracellular calcium content was significantly lower in roots of osclp as compared with the wild‐type.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Exogenous Ca 2+ restored primary and lateral root growth in osclp.
- Taxon
- riceOryza sativae1
- Material
- osclpe0
- Treatment
- Exogenous application of Ca 2+e0
- Outcome
- primary and lateral root growthe0
- Direction
- recoverye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of Ca 2+ resulted in successful recovery of both primary and lateral root growth in osclp .”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Overexpression of OsCLP improved growth.
- Taxon
- riceOryza sativae1
- Treatment
- overexpression of OsCLPe0
- Gene
- OsCLPe0
- Outcome
- growthe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Overexpression of OsCLP modified seed shape.
- Taxon
- riceOryza sativae1
- Treatment
- overexpression of OsCLPe0
- Gene
- OsCLPe0
- Outcome
- modified seed shapee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Overexpression of OsCLP modified starch structure.
- Taxon
- riceOryza sativae1
- Treatment
- overexpression of OsCLPe0
- Gene
- OsCLPe0
- Outcome
- modified seed shape and starch structuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
Overall yield was unaffected by OsCLP overexpression.
- Taxon
- riceOryza sativae1
- Treatment
- overexpression of OsCLPe0
- Gene
- OsCLPe0
- Outcome
- overall yielde0
- Direction
- remained unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected.”
e1“OsCLP ‐mediated rice growth retardation”
Claim · model-written summary
The authors conclude that OsCLP regulates intracellular calcium concentrations during rice growth.
- Taxon
- riceOryza sativae0
- Gene
- OsCLPe0
- Outcome
- rice growthe0
- Qualifier
- regulating the intracellular calcium concentrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCLP in rice growth by regulating the intracellular calcium concentrations.”
The same questions in other papers
calcium(2+) · growth · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.