Abstract
Brassinolide (BL) alleviates salt injury in cotton seedlings; however, little is known about the molecular mechanisms of this response. In this study, digital gene expression analysis was performed to better understand the regulatory pathways of BL in NaCl-stressed cotton (Gossypium hirsutum L.). Compared with control plants (CK), a total of 1 162 and 7 659 differentially expressed genes (DEGs) were detected in the leaves and roots of NaCl-treated plants, respectively. Most of the DEGs in NaCl-treated plants, compared to CK, were regulated by BL. Moreover, expression patterns of DEGs in BL+NaCl treated plants were similar to those in CK plants; however, the responses of DEGs in the leaves and roots of NaCl-treated plants to BL differed. In the roots, BL-regulated DEGs were involved in protein biosynthesis, whereas in the leaves, BL promoted photosynthesis in NaCl-stressed cotton. BL treatment also significantly increased the overall biomass, chlorophyll a + b content in leaves, and the protein content in roots in NaCl-stressed cotton. The downregulation of stress-responsive genes in BL+NaCl-stressed leaves was also found. These results suggest that BL can alleviate NaCl injury in cotton plants.
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Claims 11
Claim · model-written summary
NaCl treatment yielded 1 162 leaf DEGs relative to controls.
- Taxon
- cotton
Gossypium hirsutumt0 - Organ / stage
- leavese0
- Treatment
- NaCl-treatede0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 1 162e0
- Comparator
- control plants (CK)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with control plants (CK), a total of 1 162 and 7 659 differentially expressed genes (DEGs) were detected in the leaves and roots of NaCl-treated plants, respectively”
t0“cotton”
Claim · model-written summary
NaCl treatment yielded 7 659 root DEGs relative to controls.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- rootse0
- Treatment
- NaCl-treatede0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 7 659e0
- Comparator
- control plants (CK)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with control plants (CK), a total of 1 162 and 7 659 differentially expressed genes (DEGs) were detected in the leaves and roots of NaCl-treated plants, respectively”
t0“cotton”
Claim · model-written summary
BL regulated most NaCl responsive DEGs.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BLe0
- Outcome
- DEGs in NaCl-treated plantse0
- Comparator
- CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of the DEGs in NaCl-treated plants, compared to CK, were regulated by BL”
t0“cotton”
Claim · model-written summary
BL plus NaCl DEGs had expression patterns similar to controls.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BL+NaCle0
- Outcome
- expression patterns of DEGse0
- Comparator
- CK plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression patterns of DEGs in BL+NaCl treated plants were similar to those in CK plants”
t0“cotton”
Claim · model-written summary
Leaves and roots differed in their DEG responses to BL under NaCl.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- leaves and rootse0
- Treatment
- BLe0
- Outcome
- responses of DEGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the responses of DEGs in the leaves and roots of NaCl-treated plants to BL differed”
t0“cotton”
Claim · model-written summary
BL regulated root DEGs involved in protein biosynthesis.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- rootse0
- Treatment
- BLe0
- Outcome
- DEGs were involved in protein biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the roots, BL-regulated DEGs were involved in protein biosynthesis”
t0“cotton”
Claim · model-written summary
BL promoted photosynthesis in NaCl stressed cotton leaves.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- leavese0
- Treatment
- BLe0
- Outcome
- photosynthesise0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the leaves, BL promoted photosynthesis in NaCl-stressed cotton”
Claim · model-written summary
BL significantly increased biomass in salt stressed cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- BL treatmente0
- Outcome
- overall biomasse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BL treatment also significantly increased the overall biomass, chlorophyll a + b content in leaves, and the protein content in roots in NaCl-stressed cotton”
Claim · model-written summary
BL significantly increased leaf chlorophyll a + b in salt stressed cotton.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- leavese0
- Treatment
- BL treatmente0
- Outcome
- chlorophyll a + b contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BL treatment also significantly increased the overall biomass, chlorophyll a + b content in leaves, and the protein content in roots in NaCl-stressed cotton”
Claim · model-written summary
BL significantly increased root protein content in salt stressed cotton.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- rootse0
- Treatment
- BL treatmente0
- Outcome
- protein contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BL treatment also significantly increased the overall biomass, chlorophyll a + b content in leaves, and the protein content in roots in NaCl-stressed cotton”
Claim · model-written summary
Stress responsive genes were downregulated in BL plus NaCl leaves.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- leavese0
- Treatment
- BL+NaCle0
- Outcome
- stress-responsive genese0
- Direction
- downregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The downregulation of stress-responsive genes in BL+NaCl-stressed leaves was also found”
t0“cotton”
The same questions in other papers
brassinolide · growth · Gossypium hirsutum (cotton)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.