Paper · 2012 · Archives of applied science research

Effects of Moringa oleifera leaf extract and sodium hypochlorite seed pretreatment on seed germination, seedling growth rate and fungal abundance in two accessions of Abelmoschus esculentus (L) Moench

Nwangburuka; K. O. Oyekale; Ezekiel; et al.

8
claims
18
verbatim quotes
10
citations
2
research areas
—
Not open access

Abstract

Effects of Moringa oleifera leaf extract and Sodium hypochlorite seed pre-treatment on the seed germination, seedling growth rate and fungal activities in two accessions of Abelmoschus esculentus was examined in the Crop laboratory of Babcock University. Seed viability and seedling vigour were assessed according to ISTA, 1985; while the blotter paper method was used to determine the seed microflora. Mycological analysis was carried out by isolating and identifying fungal flora on potato dextrose agar plates. Okra seeds of CCN2005/2 and Clemson spineless varieties were pre-treated with 2.5%, 5% and 10% of Moringa leaf extract; while Sodium hypochlorite, NaOCl, was administered at 4%, 5% and 6%. A control with no seed pre-treatment was also included. Ten viable seeds, randomly selected from the seed lots of each accession, were dipped into treatment solutions for five minutes, after which it was withdrawn and prepared for further tests. Germination tests were carried out on 85mm diameter petri dishes, lined with moistened Whattman filter paper; with three replications for each treatment in a Completely Randomized Design (CRD). The two okra accessions reacted differently to different concentrations of NaOCl and Moringa leaf extract. The accessions were not significantly different for the seed viability and seedling vigour variables evaluated. Results further reveal that 4% and 6% of NaOCl inhibited the population of fungal growth; while Moringa extract reduced fungal growth and population on both accessions. Fusarium had the highest (67.5%) occurrence on seed fungal population. Penicillium however had the lowest (3.5%). This study explained the fact that pre-treatment of seeds before storage with Moringa reduces the possibility of fungal infection and also maintains the viability and vigour of the seed for a particular time period, depending on the seed type.

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Claims 8

Observationobservedoriginal resultmixedmeasured

Claim · model-written summary

The two okra accessions reacted differently to the NaOCl and Moringa leaf extract concentrations.

Treatment
NaOCl and Moringa leaf extracte1
Outcome
reacted differentlye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e1

“The two okra accessions reacted differently to different concentrations of NaOCl and Moringa leaf extract”

✓ located verbatim in the source · abstract, characters 1143–1248 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The accessions did not differ significantly in seed viability and seedling vigour.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Outcome
seed viability and seedling vigoure2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e2

“The accessions were not significantly different for the seed viability and seedling vigour variables evaluated”

✓ located verbatim in the source · abstract, characters 1250–1360 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaOCl at 4% and 6% inhibited the seed fungal population.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Treatment
4% and 6% of NaOCle3
Outcome
population of fungal growthe3
Direction
inhibitede3
Comparator
control with no seed pre-treatmente5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e3

“Results further reveal that 4% and 6% of NaOCl inhibited the population of fungal growth”

✓ located verbatim in the source · abstract, characters 1362–1450 · show in text ↑
e5

“A control with no seed pre-treatment was also included”

✓ located verbatim in the source · abstract, characters 703–757 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Moringa leaf extract reduced fungal growth and population on both okra accessions.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Treatment
Moringa extracte4
Outcome
fungal growth and populatione4
Direction
reducede4
Comparator
control with no seed pre-treatmente5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e4

“Moringa extract reduced fungal growth and population on both accessions”

✓ located verbatim in the source · abstract, characters 1458–1529 · show in text ↑
e5

“A control with no seed pre-treatment was also included”

✓ located verbatim in the source · abstract, characters 703–757 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fusarium had the highest occurrence (67.5%) on the seed fungal population.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Organ / stage
seede6
Interactor
FusariumFusariume6other
Outcome
occurrencee6
Value
67.5%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Fusarium
Fusarium · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e6

“Fusarium had the highest (67.5%) occurrence on seed fungal population. Penicillium however had the lowest (3.5%)”

✓ located verbatim in the source · abstract, characters 1531–1643 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Penicillium had the lowest occurrence (3.5%) on the seed fungal population.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Organ / stage
seede6
Interactor
PenicilliumPenicilliume6other
Outcome
occurrencee6
Value
3.5%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗
Penicillium
Penicillium · Interactor · picture from its Wikimedia Commons categoryMilo Manica · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e6

“Fusarium had the highest (67.5%) occurrence on seed fungal population. Penicillium however had the lowest (3.5%)”

✓ located verbatim in the source · abstract, characters 1531–1643 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Pre-treatment of seeds with Moringa reduces the possibility of fungal infection.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Treatment
pre-treatment of seeds before storage with Moringae7
Outcome
possibility of fungal infectione7
Direction
reducese7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e7

“pre-treatment of seeds before storage with Moringa reduces the possibility of fungal infection and also maintains the viability and vigour of the seed for a particular time period, depending on the seed type”

✓ located verbatim in the source · abstract, characters 1680–1887 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Pre-treatment with Moringa maintains seed viability and vigour for a period that depends on seed type.

Taxon
Abelmoschus esculentusAbelmoschus esculentuse0
Treatment
pre-treatment of seeds before storage with Moringae7
Outcome
viability and vigoure7
Direction
maintainse7
Qualifier
for a particular time period, depending on the seed typee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“in two accessions of Abelmoschus esculentus was examined”

✓ located verbatim in the source · abstract, characters 152–208 · show in text ↑
e7

“pre-treatment of seeds before storage with Moringa reduces the possibility of fungal infection and also maintains the viability and vigour of the seed for a particular time period, depending on the seed type”

✓ located verbatim in the source · abstract, characters 1680–1887 · show in text ↑

storage · growth · Abelmoschus esculentus (okra)Positive and negative results

9 papers: 7 positive, 2 negative or no effect

6 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

5 papers more in this group

See all 9 papers →

botanical · disease · Abelmoschus esculentus (okra)Positive and negative results

18 papers: 16 positive, 2 negative or no effect

15 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

14 papers more in this group

See all 18 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.