Abstract
The influence of the basal medium and different plant growth regulators on the efficient micropropagation of nodal explants from mature trees of sour orange and ‘Cleopatra’ mandarin citrus rootstocks was studied. The preference of the two citrus rootstock shoot cultures for high-salt media, like MS or DKW, was observed. Several combinations of BA and AD, KIN or GA were made in an attempt to optimize the proliferation phase. The addition of BA and AD to the culture medium improved the proliferation in sour orange or ‘Cleopatra’ mandarin in the same way as BA/GA. The use of different combinations of BA/KIN did not improve any of the studied variables. The transfer of in vitro shoots to rooting media, containing different concentrations of IBA and IAA, produced complete plantlets of ‘Cleopatra’ mandarin, but very low rooting percentages in sour orange were obtained. To improve the rooting results, different combinations of NAA and IBA were tested and higher rooting percentages than in the IBA/IAA combinations were produced. In sour orange nearly 100% of rooted explants were obtained.
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Claims 8
Claim · model-written summary
Both citrus rootstock shoot cultures preferred high-salt MS or DKW media.
CitrusNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The preference of the two citrus rootstock shoot cultures for high-salt media, like MS or DKW, was observed”
t0“CITRUS ROOTSTOCKS”
Claim · model-written summary
BA and AD improved proliferation in sour orange.
- Taxon
- sour orange
Citrus ×aurantiume0 - Treatment
- BA and ADe0
- Outcome
- proliferatione0
- Direction
- improvede0
- Comparator
- BA/GAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of BA and AD to the culture medium improved the proliferation in sour orange or ‘Cleopatra’ mandarin in the same way as BA/GA”
Claim · model-written summary
BA and AD improved proliferation in Cleopatra mandarin.
Verbatim
e0“The addition of BA and AD to the culture medium improved the proliferation in sour orange or ‘Cleopatra’ mandarin in the same way as BA/GA”
Claim · model-written summary
BA/KIN combinations did not improve the studied variables.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of different combinations of BA/KIN did not improve any of the studied variables”
t0“CITRUS ROOTSTOCKS”
Claim · model-written summary
IBA and IAA rooting media produced complete Cleopatra mandarin plantlets.
- Taxon
- ‘Cleopatra’ mandarine0
- Treatment
- rooting media, containing different concentrations of IBA and IAAe0
- Outcome
- complete plantletse0
Not reportedComparatorDirection
Verbatim
e0“The transfer of in vitro shoots to rooting media, containing different concentrations of IBA and IAA, produced complete plantlets of ‘Cleopatra’ mandarin”
Claim · model-written summary
IBA and IAA rooting media gave very low rooting in sour orange.
- Taxon
- sour orangeCitrus ×aurantiume0
- Outcome
- rooting percentagese0
- Direction
- very lowe0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“very low rooting percentages in sour orange were obtained”
t0“CITRUS ROOTSTOCKS”
Claim · model-written summary
NAA and IBA combinations gave higher rooting than IBA and IAA combinations.
- Treatment
- different combinations of NAA and IBAe0
- Outcome
- rooting percentagese0
- Direction
- highere0
- Comparator
- IBA/IAA combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“different combinations of NAA and IBA were tested and higher rooting percentages than in the IBA/IAA combinations were produced”
t0“CITRUS ROOTSTOCKS”
Claim · model-written summary
Nearly all sour orange explants rooted under the improved rooting approach.
- Taxon
- sour orangeCitrus ×aurantiume0
- Outcome
- rooted explantse0
- Value
- nearly 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In sour orange nearly 100% of rooted explants were obtained”
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