Abstract
Received: 2016-11-23 | Accepted: 2016-04-14 | Available online: 2016-12-22 http://dx.doi.org/10.15414/afz.2016.19.04.157-162 The objective of this research was to investigate possible genetic variation in the sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying. The work confirmed that the selected physiological characteristics (RWC, osmotic potential, stress index and created nodules on roots) are good evaluating parameters for the determination of water stress in plant. In the floricultural year 2014 an experiment with four genetic resources of soybean was launched. Sowing of Maverick (USA), Drina (HRV), Nigra (SVK) and Polanka (CZK) genotypes was carried out in the containers of 15 l capacity. This stress had a negative impact on the physiological parameters. By comparing the RWC values, the decrease was more significant at the end of dehydration, which was monitored in Maverick and Drina genotypes using the Nitrazon inoculants and water stress effect. Inoculated stressed Nigra and Polanka genotypes have kept higher water content till the end of dehydration period. Also the proline accumulation was monitored during the water stress, whilst higher content of free proline reached of Maverick. More remarkable decrease of osmotic potential was again registered in a foreign Drina and Maverick genotypes in the inoculated variations. Nigra and Polanka genotypes responses not so significant in the given conditions. Key words: soybean, drought, stress, inoculation of Nitrazon References CANDRÁKOVÁ, E. and LÍŠKA, E. (2006) Vplyv teplotných a vlahových podmienok na tvorbu úrody hrachu siateho, Influence of Temperature and Water Conditions on Field Pea Yield Formation. Acta fytotechnica et zootechnica, vol. 9, no. 3, pp. 57-61. DOWNIE, J. A. (1998) Functions of rhizobial nodulation genes. In Spaink, H. P., Kondorosi A. and Hooykaas, P. J. J. (ed.), Rhizobiacee. Dordrecht: Kluwer Academic Publisher, pp. 387. FAROOQ, M. et. al. (2009) Plant drought stress: effects, mechanisms and management. Journal of Sustainable Agriculture, pp. 153–188. doi: http://dx.doi.org/10.1080/10440040802548555 FISCHER, R.A. and MAURER, R. (1978) Drought resistance in spring wheat cultivars. I. Grain yield response. Aust. J. Agric. Res., vol. 29, pp. 897–907. doi: http://dx.doi.org/10.1071/AR9780897 FREDERICK, J.R., CAMP, C.R. and BAUER, P.J. (2001) Drought-stress effects on branch and mainstem seed yield and yield components of determinate soybean. Crop Science, vol. 41, no. 3, pp. 759-763. doi: http://dx.doi.org/10.2135/cropsci2001 JAMES, A. T., LAWN R. J. and COOPER, M. (2008) Genotypic variation for drought stress response traits in soybean. I. Variation in soybean and wild Glycine spp. for epidermal conductance, osmotic potential, and relative water content. Australian Journal of Agricultural Research, vol. 59, no.7, pp. 656–669. doi: http://dx.doi.org/10.1071/AR07159 KU, Y. S., AU-YEUNG, W. K. and YUNG, Y. L. (2013) Drought Stress and Tolerance in Soybean. Chapter 10. http://dx.doi.org/ 10.5772/52945 LEPORT, L. et aL. (2003) Physiological responses of cool-season grain legumes to drought in the lowrainfall Mediterranean environment of south-western Australia. In: N.P. Saxena (Ed.), Management of Agricultural Drought: Agronomic and Genetic Options, pp. 163–172, Enfield: Science Publishers. MALHOTRA, R.S., SARKER, A. and SAXENA, M.C. (2004) Drought tolerance in chickpea and lentil-present status and future strategies. Challenges and Strategies for Dryland Agriculture, no. 32, pp. 257–273. MIRABELLLA, R., (2004) Role of ROP GTPases and NodFactor Signaling in Medicago Root Nodule Infection: Thesis. Wageningen: Wageningen University, 160p. PATTERSON, T.G. and LARNE, T.A. (1983) Nitrogen fixation by soybeans. Seasonal and cultivar effects, and comparison of estimates. Crop Science, vol. 23, no.3, pp. 488-492. doi: http://dx.doi.org/10.2135/cropsci1983.0011183X002300030012x RASAEI, B., GHOBADI, M.E. and AMIRI, M. K. (2013) Effect of osmotic potential on germination and seedling characteristics of soybean seeds. International Journal of Agriculture and Crop Sciences. vol., 5, no.11, 1265-1268. RICCARDI, L.,POLIGNANO, G.B. and DE GIOVANNI, C. (2001) Genotypic response of Faba bean to water stress. Euphytica, vol.118, no.1. pp. 39–46. doi: http://dx.doi.org/10.1023/A:1004078017159 SANCHOLI, G., MOBASSER, H. R. and FANAEI, H. R. (2015) Effect Inoculation of Soybean Cultivars with bacteria Rhizobium japonicum in Sistan. Biological Forum – An International Journal no.1, pp. 552-558. ISSN 0975-1130. SADEGHIPOUR, O. and ABBASI, S. (2012) Soybean response to drought and seed inoculation. World Applied Sciences Journal, vol.1, no.1, pp. 55-60. doi: http://dx.doi.org/10.1111/j.1467-6486.2004.00462.x VIRGINIA, S., PAGAN, M. and COOPER, M. (2012) Genetic Analysis of Relative Water Content (RWC) in Two Recombinant Inbred Line Populations of Soybean [Glycine max (L.) Merr.] Journal of Plant Genome Sciences, vol. 1, no. 2. doi: http://dx.doi.org/10.5147/jpgs.2012.0058
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Claims 7
Claim · model-written summary
Water stress had a negative impact on the physiological parameters of soybean.
- Taxon
- soybean
Glycine maxe1 - Treatment
- soil dryinge1
- Outcome
- physiological parameterse2
- Direction
- negative impacte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e2“This stress had a negative impact on the physiological parameters”
Claim · model-written summary
Relative water content decreased more significantly at the end of dehydration in Maverick.
- Taxon
- soybean
Glycine maxe1 - Material
- Mavericke4
- Treatment
- dehydratione3
- Outcome
- RWC valuese3
- Direction
- decreasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e3“By comparing the RWC values, the decrease was more significant at the end of dehydration”
e4“which was monitored in Maverick”
Claim · model-written summary
Inoculated and stressed Nigra and Polanka kept higher water content until the end of dehydration.
- Taxon
- soybean
Glycine maxe1 - Material
- Nigrae7
- Treatment
- Inoculated stressede6
- Outcome
- water contente5
- Direction
- highere5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e5“have kept higher water content till the end of dehydration period”
e6“Inoculated stressed”
e7“Nigra (SVK) and Polanka (CZK) genotypes”
Claim · model-written summary
Free proline content was higher in Maverick under water stress.
- Taxon
- soybean
Glycine maxe1 - Material
- Mavericke2
- Treatment
- water stresse2
- Outcome
- content of free prolinee2
- Direction
- highere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e2“Also the proline accumulation was monitored during the water stress, whilst higher content of free proline reached of Maverick”
Claim · model-written summary
Osmotic potential decreased more remarkably in Drina under inoculated conditions.
- Taxon
- soybean
Glycine maxe1 - Material
- Drinae3
- Treatment
- inoculatede3
- Outcome
- osmotic potentiale2
- Direction
- decreasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e2“More remarkable decrease of osmotic potential”
e3“Drina and Maverick genotypes in the inoculated variations”
Claim · model-written summary
Responses of Nigra and Polanka were not significant under the given conditions.
- Taxon
- soybean
Glycine maxe1 - Material
- Nigrae7
- Outcome
- responsese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e7“Nigra (SVK) and Polanka (CZK) genotypes”
e2“responses not so significant in the given conditions”
Claim · model-written summary
RWC, osmotic potential, stress index and nodule number were good evaluating parameters for water stress in soybean.
- Taxon
- soybean
Glycine maxe1 - Outcome
- determination of water stresse2
- Value
- good evaluating parameterse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying”
e2“The work confirmed that the selected physiological characteristics (RWC, osmotic potential, stress index and created nodules on roots) are good evaluating parameters for the determination of water stress in plant”
The same questions in other papers
inoculation · soil: physics · Glycine max (soybean)Results agree
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- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.