Abstract
Alfalfa (Medicago sativa) is difficult to ensile successfully because of the low content of moisture and water-soluble carbohydrates (WSC) in fresh alfalfa and the high buffering capacity in fresh alfalfa. Here, we conducted a study to evaluate the effects of three lactic acid bacteria (LAB) inoculants (Lactobacillus casei, Lactobacillus plantarum, and Pediococcus pentosaceus) on silage quality, in situ ruminal degradability, and in vitro fermentation of alfalfa silage. The first cut of alfalfa was wilted, chopped, and randomly divided into four groups: the control (CON) and control mixed with three separate LAB inoculants (106 cfu g−1). Simmental steers with a body weight of (452±18) kg and with installed rumen fistulas were prepared for in situ degradation and for in vitro gas production. LAB inoculants had a lower (P<0.05) content of butyric acid than the CON group. Among them, the L. casei inoculated silage had a higher (P<0.05) content of water-soluble carbohydrate (WSC) and a lower (P<0.05) NH3-H content. The effective degradation (ED) of crude protein in LAB inoculation decreased (P<0.05), while the ED of acid detergent fiber increased (P<0.05) in situ fermentation. The alfalfa silage with LAB inoculants produced more carbon dioxide (P<0.05). The NH3-H content of mixed incubation fluid in L. casei inoculated silage was lower (P<0.05) compared with other groups. Therefore, this study showed that LAB inoculants could improve both ensiling quality and degradation. In particular, the L. casei inoculations exhibited better performance by limiting proteolysis during ensiling.
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Claims 8
Claim · model-written summary
LAB inoculants had a lower butyric acid content than the control silage.
- Taxon
- Alfalfa
Medicago sativae0 - Treatment
- LAB inoculantse1
- Interactor
- LABe1other
- Outcome
- content of butyric acide1
- Direction
- lowere1
- Comparator
- CON groupe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e1“LAB inoculants had a lower (P<0.05) content of butyric acid than the CON group”
Claim · model-written summary
The L. casei inoculated silage had a higher water-soluble carbohydrate (WSC) content.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- L. casei inoculated silagee2
- Interactor
- L. casei
Lacticaseibacillus caseie2other - Outcome
- water-soluble carbohydrate (WSC)e2
- Direction
- highere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e2“Among them, the L. casei inoculated silage had a higher (P<0.05) content of water-soluble carbohydrate (WSC) and a lower (P<0.05) NH3-H content”
Claim · model-written summary
The L. casei inoculated silage had a lower NH3-H content.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- L. casei inoculated silagee2
- Interactor
- L. caseiLacticaseibacillus caseie2other
- Outcome
- NH3-H contente2
- Direction
- lowere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e2“Among them, the L. casei inoculated silage had a higher (P<0.05) content of water-soluble carbohydrate (WSC) and a lower (P<0.05) NH3-H content”
Claim · model-written summary
LAB inoculation decreased the effective degradation of crude protein in situ.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- LAB inoculatione3
- Interactor
- LABe3other
- Outcome
- effective degradation (ED) of crude proteine3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e3“The effective degradation (ED) of crude protein in LAB inoculation decreased (P<0.05), while the ED of acid detergent fiber increased (P<0.05) in situ fermentation”
Claim · model-written summary
LAB inoculation increased the effective degradation of acid detergent fiber in situ.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- LAB inoculatione3
- Interactor
- LABe3other
- Outcome
- ED of acid detergent fibere3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e3“The effective degradation (ED) of crude protein in LAB inoculation decreased (P<0.05), while the ED of acid detergent fiber increased (P<0.05) in situ fermentation”
Claim · model-written summary
The alfalfa silage with LAB inoculants produced more carbon dioxide.
- Taxon
- alfalfaMedicago sativae4
- Treatment
- LAB inoculantse4
- Interactor
- LABe4other
- Outcome
- carbon dioxidee4
- Direction
- moree4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The alfalfa silage with LAB inoculants produced more carbon dioxide (P<0.05)”
Claim · model-written summary
The NH3-H content of mixed incubation fluid was lower in L. casei inoculated silage than in other groups.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- L. casei inoculated silagee5
- Interactor
- L. caseiLacticaseibacillus caseie5other
- Outcome
- NH3-H content of mixed incubation fluide5
- Direction
- lowere5
- Comparator
- other groupse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e5“The NH3-H content of mixed incubation fluid in L. casei inoculated silage was lower (P<0.05) compared with other groups”
Claim · model-written summary
L. casei inoculations performed better by limiting proteolysis during ensiling.
- Taxon
- AlfalfaMedicago sativae0
- Treatment
- L. casei inoculationse6
- Interactor
- L. caseiLacticaseibacillus caseie6other
- Outcome
- performancee6
- Qualifier
- by limiting proteolysis during ensilinge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alfalfa (Medicago sativa) is difficult to ensile successfully”
e6“the L. casei inoculations exhibited better performance by limiting proteolysis during ensiling”
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