Abstract
Efforts were made to develop a culture medium for successful in vitro propagation of radish. The effect of various carbon sources such as sucrose, glucose, maltose and dextrose were investigated on in vitro shoot regeneration of Rabu (Raphanus sativus L.) Var. Beeralu using aseptic hypocotyl explants. Numbers of regenerated shoots were evaluated one month after establishment. Test shows that there were significant effects at p<0.05 level on radish shoots, regenerated in different carbon sources. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used. The maximum number of shoots (6 shoots/explant) on MS medium supplemented with 3% dextrose and maximum shoot length (4cm) was obtained by 4% dextrose. Regeneration frequency was 100% in 4% dextrose. The least number of shoots were observed by each and every concentration of glucose except 4%. Among the four types of carbon sources that were employed in the present study, dextrose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, maltose and glucose from hypocotyl explants of Raphanus sativus L Var. Beeralu Rabu.
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Claims 6
Claim · model-written summary
Carbon source type and concentration significantly influenced radish shoot regeneration, growth and multiplication.
- Taxon
- radish
Raphanus sativuse0 - Treatment
- type and concentration of carbon source usede0
- Outcome
- frequency, growth and multiplication ratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Test shows that there were significant effects at p<0.05 level on radish shoots, regenerated in different carbon sources. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used”
Claim · model-written summary
MS medium with 3% dextrose gave the maximum of 6 shoots per explant.
- Taxon
- radishRaphanus sativust0
- Treatment
- MS medium supplemented with 3% dextrosee0
- Outcome
- number of shootse0
- Direction
- maximume0
- Value
- 6 shoots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum number of shoots (6 shoots/explant) on MS medium supplemented with 3% dextrose”
t0“Radish”
Claim · model-written summary
Four percent dextrose gave the maximum shoot length of 4cm.
- Taxon
- radishRaphanus sativust0
- Treatment
- 4% dextrosee0
- Outcome
- shoot lengthe0
- Direction
- maximume0
- Value
- 4cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum shoot length (4cm) was obtained by 4% dextrose”
t0“Radish”
Claim · model-written summary
Four percent dextrose gave 100% regeneration frequency.
- Taxon
- radishRaphanus sativust0
- Treatment
- 4% dextrosee0
- Outcome
- Regeneration frequencye0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regeneration frequency was 100% in 4% dextrose”
t0“Radish”
Claim · model-written summary
Glucose concentrations other than 4% gave the fewest shoots.
- Taxon
- radishRaphanus sativust0
- Treatment
- glucosee0
- Outcome
- number of shootse0
- Direction
- leaste0
- Qualifier
- each and every concentration of glucose except 4%e0
- Comparator
- 4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The least number of shoots were observed by each and every concentration of glucose except 4%”
t0“Radish”
Claim · model-written summary
Four percent dextrose was judged the best carbon source for multiple shoot regeneration, followed by sucrose, maltose and glucose.
- Taxon
- Raphanus sativus L Var. Beeralu RabuRaphanus sativuse0
- Treatment
- dextrose at 4%e0
- Outcome
- better choice for multiple shoot regeneratione0
- Comparator
- sucrose, maltose and glucosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dextrose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, maltose and glucose from hypocotyl explants of Raphanus sativus L Var. Beeralu Rabu”
The same questions in other papers
glucose · growth · Raphanus sativus (radish)Results agree
1 paper in the same direction as this paper
- negative
in vitro · growth · Raphanus sativus (radish)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.