Abstract
Induction of secreted and intracellular purple acid phosphatases (PAPs; EC 3.1.3.2) is widely recognized as an adaptation of plants to phosphorus (P) deficiency. The secretion of PAPs plays important roles in P acquisition. However, little is known about the functions of intracellular PAP in plants and nodules. In this study, we identified a novel PAP gene GmPAP21 in soybean. Expression of GmPAP21 was induced by P limitation in nodules, roots and old leaves, and increased in roots with increasing duration of P starvation. Furthermore, the induction of GmPAP21 in nodules and roots was more intensive than in leaves in both P-efficient genotype HN89 and P-inefficient genotype HN112 in response to P starvation, and the relative expression in the leaves and nodules of HN89 was significantly greater than that of HN112 after P deficiency treatment. Further functional analyses showed that over-expressing GmPAP21 significantly enhanced both acid phosphatase activity and growth performance of hairy roots under P starvation condition, indicating that GmPAP21 plays an important role in P utilization. Moreover, GUS expression driven by GmPAP21 promoter was shown in the nodules besides roots. Overexpression of GmPAP21 in transgenic soybean significantly inhibited nodule growth, and thereby affected plant growth after inoculation with rhizobia. This suggests that GmPAP21 is also possibly involved in regulating P metabolism in nodules. Taken together, our results suggest that GmPAP21 is a novel plant PAP that functions in the adaptation of soybean to P starvation, possibly through its involvement in P recycling in plants and P metabolism in nodules.
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Claims 10
Claim · model-written summary
GmPAP21 was identified as a novel soybean PAP gene.
- Taxon
- soybean
Glycine maxt0 - Gene
- GmPAP21e0
- Outcome
- novel PAP gene GmPAP21e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“we identified a novel PAP gene GmPAP21 in soybean”
Claim · model-written summary
P limitation induced GmPAP21 expression in nodules, roots and old leaves.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- nodules, roots and old leavese0
- Treatment
- P limitatione0
- Gene
- GmPAP21e0
- Outcome
- Expression of GmPAP21e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Expression of GmPAP21 was induced by P limitation in nodules, roots and old leaves”
Claim · model-written summary
Root GmPAP21 expression increased with P starvation duration.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- rootse0
- Treatment
- P starvatione0
- Gene
- GmPAP21e0
- Outcome
- Expression of GmPAP21e0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Expression of GmPAP21 was induced by P limitation in nodules, roots and old leaves, and increased in roots with increasing duration of P starvation”
Claim · model-written summary
P starvation induced GmPAP21 more in nodules and roots than leaves of HN89 and HN112.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- nodules and rootse0
- Treatment
- P starvatione0
- Gene
- GmPAP21e0
- Outcome
- induction of GmPAP21e0
- Direction
- more intensivee0
- Comparator
- leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“the induction of GmPAP21 in nodules and roots was more intensive than in leaves in both P-efficient genotype HN89 and P-inefficient genotype HN112 in response to P starvation”
Claim · model-written summary
Following P deficiency, HN89 leaf and nodule GmPAP21 expression exceeded HN112.
- Taxon
- soybeanGlycine maxt0
- Material
- HN89e0
- Organ / stage
- leaves and nodulese0
- Treatment
- P deficiency treatmente0
- Gene
- GmPAP21e0
- Outcome
- relative expressione0
- Direction
- greatere0
- Comparator
- HN112e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“the induction of GmPAP21 in nodules and roots was more intensive than in leaves in both P-efficient genotype HN89 and P-inefficient genotype HN112 in response to P starvation, and the relative expression in the leaves and nodules of HN89 was significantly greater than that of HN112 after P deficiency treatment”
Claim · model-written summary
GmPAP21 overexpression significantly increased acid phosphatase activity of hairy roots under P starvation.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- hairy rootse0
- Treatment
- over-expressing GmPAP21e0
- Gene
- GmPAP21e0
- Outcome
- acid phosphatase activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“over-expressing GmPAP21 significantly enhanced both acid phosphatase activity and growth performance of hairy roots under P starvation condition”
Claim · model-written summary
GmPAP21 overexpression significantly improved hairy root growth under P starvation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- over-expressing GmPAP21e0
- Gene
- GmPAP21e0
- Outcome
- growth performance of hairy rootse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“over-expressing GmPAP21 significantly enhanced both acid phosphatase activity and growth performance of hairy roots under P starvation condition”
Claim · model-written summary
The GmPAP21 promoter drove GUS expression in nodules and roots.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- nodules besides rootse0
- Gene
- GmPAP21e0
- Outcome
- GUS expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“GUS expression driven by GmPAP21 promoter was shown in the nodules besides roots”
Claim · model-written summary
GmPAP21 overexpression inhibited nodule growth in transgenic soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Overexpression of GmPAP21e0
- Gene
- GmPAP21e0
- Outcome
- nodule growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Overexpression of GmPAP21 in transgenic soybean significantly inhibited nodule growth”
Claim · model-written summary
After rhizobia inoculation, GmPAP21 overexpression affected plant growth.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Overexpression of GmPAP21e0
- Interactor
- rhizobiae0symbiont
- Gene
- GmPAP21e0
- Outcome
- plant growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean”
e0“Overexpression of GmPAP21 in transgenic soybean significantly inhibited nodule growth, and thereby affected plant growth after inoculation with rhizobia”
The same questions in other papers
phosphorus · molecular: expression · Glycine max (soybean)Positive and negative results
5 papers in the same direction as this paper · 7 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- negative
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.