Paper · 2016 · Grass and Forage Science

Methylotrophic yeast, lactic acid bacteria and glycerine as additives for sugarcane silage

B. F. Carvalho; C. L. S. Ávila; M. N. Pereira; et al.

7
claims
17
verbatim quotes
9
citations
2
research areas
—
Not open access

Abstract

Abstract The ensiling of sugarcane results in high dry‐matter ( DM ) loss, but the addition of glycerine may compensate for the loss during ensiling. Methanol is the most undesirable contaminant of crude glycerine destined for animal feeding. The aim of this study was to evaluate the ability of the yeast strain Pichia methanolica NCYC 1381 to reduce the methanol concentration in sugarcane silage inoculated with Lactobacillus hilgardii CCMA 0170 + glycerine. A randomized design consisted of four dose rates of glycerine (0, 4, 8 and 12% of fresh forage), three periods of silage fermentation (11, 34 and 68 d) and three combinations of microbial additives [ L. hilgardii ( LH ), L. hilgardii plus P. methanolica ( LH + PM ) and without microbial additive ( WI )]. The DM of the fresh sugarcane was 275 g kg −1 . The linear reduction in neutral detergent fibre caused by glycerine inclusion was probably due to a dilution effect. The LH treatment increased the concentrations of the succinic, acetic and propionic acids, and 1,2‐propanediol, and reduced the yeast population. The LH + PM treatment increased DM loss of sugarcane silage with 12% glycerine and L. hilgardii CCMA 0170 (6·1 log cfu g −1 of FM ) reduced the DM loss when compared to the silage without additives. Under the conditions of the experiment, the P. methanolica treatment did not reduce the methanol concentration in silage.

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Claims 7

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Glycerine inclusion linearly reduced neutral detergent fibre in sugarcane silage.

Taxon
sugarcanet0
Treatment
glycerine inclusione0
Outcome
neutral detergent fibree0
Direction
reductione0

Not reportedComparator

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e0

“The linear reduction in neutral detergent fibre caused by glycerine inclusion was probably due to a dilution effect”

✓ located verbatim in the source · abstract, characters 816–931 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The LH treatment increased the concentrations of succinic, acetic and propionic acids in sugarcane silage.

Taxon
sugarcanet0
Treatment
LHe0
Outcome
concentrations of the succinic, acetic and propionic acidse0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e0

“The LH treatment increased the concentrations of the succinic, acetic and propionic acids”

✓ located verbatim in the source · abstract, characters 933–1022 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The LH treatment increased 1,2-propanediol in sugarcane silage.

Taxon
sugarcanet0
Treatment
LHe0
Outcome
propanediole1
Direction
increasede0

Not reportedComparator

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e0

“The LH treatment increased the concentrations of the succinic, acetic and propionic acids”

✓ located verbatim in the source · abstract, characters 933–1022 · show in text ↑
e1

“propanediol”

✓ located verbatim in the source · abstract, characters 1032–1043 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The LH treatment reduced the yeast population in sugarcane silage.

Taxon
sugarcanet0
Treatment
LHe0
Interactor
yeaste2other
Outcome
yeast populatione2
Direction
reducede2

Not reportedComparator

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e0

“The LH treatment increased the concentrations of the succinic, acetic and propionic acids”

✓ located verbatim in the source · abstract, characters 933–1022 · show in text ↑
e2

“reduced the yeast population”

✓ located verbatim in the source · abstract, characters 1049–1077 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The LH + PM treatment with 12% glycerine increased DM loss of sugarcane silage.

Taxon
sugarcanet0
Treatment
LH + PMe3
Outcome
DM losse3
Direction
increasede3

Not reportedComparator

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e3

“The LH + PM treatment increased DM loss of sugarcane silage with 12% glycerine”

✓ located verbatim in the source · abstract, characters 1079–1157 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

L. hilgardii CCMA 0170 reduced DM loss in sugarcane silage compared with silage without additives.

Taxon
sugarcanet0
Treatment
L. hilgardii CCMA 0170e4
Interactor
L. hilgardii CCMA 0170e4other
Outcome
DM losse5
Direction
reducede5
Comparator
silage without additivese5

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e4

“with 12% glycerine and L. hilgardii CCMA 0170”

✓ located verbatim in the source · abstract, characters 1139–1184 · show in text ↑
e5

“reduced the DM loss when compared to the silage without additives”

✓ located verbatim in the source · abstract, characters 1211–1276 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The P. methanolica treatment did not reduce methanol concentration in sugarcane silage under the conditions of the experiment.

Taxon
sugarcanet0
Treatment
P. methanolicae6
Interactor
P. methanolicaOgataea methanolicae6other
Outcome
methanol concentratione6
Direction
did not reducee6
Qualifier
Under the conditions of the experimente6

Not reportedComparator

Verbatim

t0

“sugarcane silage”

✓ located verbatim in the source · title, characters 74–90 · show in text ↑
e6

“Under the conditions of the experiment, the P. methanolica treatment did not reduce the methanol concentration in silage”

✓ located verbatim in the source · abstract, characters 1278–1398 · show in text ↑