Abstract
A simple protocol for mass propagation of potato microtubers was developed using an automated low-cost bioreactor system. Microtubers of potato were induced by a two-step culture method. In the first step (step A), the stock plants were inoculated in the bioreactor for growth and multiplication of plantlets, After four weeks, the medium was replaced with a new one to proceed to step B for microtuber induction. Comparative studies between solid and bioreactor culture (continuous immersion [with net or without net] and temporary immersion in liquid medium using ebb and flood) revealed that shoot multiplication and growth were more efficient in continuous immersion (with net) bioreactor. We also studied the effect of inoculation density on potato micropropagation during bioreactor culture and maximum responses were recorded when there were 50 nodal explants per bioreactor. After shoot proliferation, the culture medium was replaced with one containing a higher concentration of sucrose, with or without 6-benzylaminopurine (BAP) and kept under dark conditions. The analysis of tuber classification according to size showed that addition of BAP in the culture medium influenced the formation of microtubers larger than 1.1 g. It has also been observed that there is a strong influence of medium renewal on individual microtuber growth during bioreactor culture of potato. The results indicate that our system could be applied for mass propagation of potato tubers at low cost.
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Claims 5
Claim · model-written summary
Continuous immersion (with net) bioreactor gave more efficient shoot multiplication and growth than solid culture.
- Taxon
- potato
Solanum tuberosume5 - Treatment
- continuous immersion (with net) bioreactore0
- Outcome
- shoot multiplication and growthe0
- Direction
- more efficiente0
- Comparator
- solide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“mass propagation of potato microtubers”
e0“Comparative studies between solid and bioreactor culture (continuous immersion [with net or without net] and temporary immersion in liquid medium using ebb and flood) revealed that shoot multiplication and growth were more efficient in continuous immersion (with net) bioreactor”
Claim · model-written summary
Maximum responses in potato micropropagation were recorded at 50 nodal explants per bioreactor.
- Taxon
- potatoSolanum tuberosume5
- Treatment
- 50 nodal explants per bioreactore1
- Outcome
- responsese1
- Direction
- maximume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“mass propagation of potato microtubers”
e1“We also studied the effect of inoculation density on potato micropropagation during bioreactor culture and maximum responses were recorded when there were 50 nodal explants per bioreactor”
Claim · model-written summary
Addition of BAP to the culture medium influenced the formation of potato microtubers larger than 1.1 g.
- Taxon
- potatoSolanum tuberosume5
- Treatment
- addition of BAPe2
- Outcome
- formation of microtubers larger than 1.1 ge2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“mass propagation of potato microtubers”
e2“addition of BAP in the culture medium influenced the formation of microtubers larger than 1.1 g”
Claim · model-written summary
Medium renewal had a strong influence on individual microtuber growth during potato bioreactor culture.
- Taxon
- potatoSolanum tuberosume5
- Treatment
- medium renewale3
- Outcome
- individual microtuber growthe3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“mass propagation of potato microtubers”
e3“there is a strong influence of medium renewal on individual microtuber growth during bioreactor culture of potato”
Claim · model-written summary
The bioreactor system could be applied for low-cost mass propagation of potato tubers.
Verbatim
e4“The results indicate that our system could be applied for mass propagation of potato tubers at low cost”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.