Paper · 1978

Yield increases of tropical grasses after inoculation with Spirillum lipoferum

R. L. Smith; S. C. Schank; J. H. Bouton; et al.

13
claims
38
verbatim quotes
29
citations
4
research areas
—
Not open access

Abstract

For three years, higher dry matter yields have been obtained from grasses inoculated with tropical strains of the bacterium Spirillum lipoferum than uninoculated counterparts. During the first year, 1974, inoculated Panicum maximum (guineagrass) and Digitada decumbens (digitgrass) produced 80% and 61% more protein than uninoculated controls. In 1975, inoculation produced significantly higher protein and dry matter yields in Pennisetum americanum (pearl millet) and P. maximum. Projected yields using regression analysis of both pearl millet and guineagrass indicated that about 40 kg N ha-1 yr1 were replaced by inoculation. Although overall production was lower during 1976, yield responses due to inoculation were similar to those of 1975 in guineagrass. Nitrogen fertilizer was required to enhance response to inoculation and higher rates of nitrogen fertilizer repressed response. Acetylene reduction verified that nitrogen fixation occurred, but did not correlate well with yield increases. Immunofluorescent techniques were used to specifically identify and monitor Spirillum lipoferum popula tions in the soil. These data are being used to evaluate inoculation procedures. Inoculation with Spirillum lipoferum has given yield increases for three consecutive years in Florida. This indicates: 1. response to inoculation can be repeated. 2. economically important amounts of nitrogen can be replaced by inoculation. 3. a high potential exists for developing grass-bacteria systems.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculated grasses had higher dry matter yields over three years.

Taxon
grassese0
Treatment
inoculated with tropical strains of the bacterium Spirillum lipoferume0
Interactor
Spirillum lipoferumAzospirillum brasilensee0symbiont
Outcome
dry matter yieldse0
Direction
highere0
Comparator
uninoculated counterpartse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“For three years, higher dry matter yields have been obtained from grasses inoculated with tropical strains of the bacterium Spirillum lipoferum than uninoculated counterparts”

✓ located verbatim in the source · abstract, characters 0–174 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculated Panicum maximum produced 80% more protein in 1974.

Taxon
Panicum maximumMegathyrsus maximuse0
Treatment
inoculatede0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
proteine0
Direction
moree0
Value
80%e0
Comparator
uninoculated controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Megathyrsus maximus
Megathyrsus maximus · TaxonHarry Rose from South West Rocks, Australia · CC BY 2.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“During the first year, 1974, inoculated Panicum maximum (guineagrass) and Digitada decumbens (digitgrass) produced 80% and 61% more protein than uninoculated controls”

✓ located verbatim in the source · abstract, characters 176–342 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculated Digitada decumbens produced 61% more protein in 1974.

Taxon
Digitada decumbense0
Treatment
inoculatede0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
proteine0
Direction
moree0
Value
61%e0
Comparator
uninoculated controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“During the first year, 1974, inoculated Panicum maximum (guineagrass) and Digitada decumbens (digitgrass) produced 80% and 61% more protein than uninoculated controls”

✓ located verbatim in the source · abstract, characters 176–342 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculation significantly increased protein and dry matter yields in pearl millet in 1975.

Taxon
Pennisetum americanumCenchrus americanuse0
Treatment
inoculatione0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
protein and dry matter yieldse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cenchrus americanus
Cenchrus americanus · TaxonPublic domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“In 1975, inoculation produced significantly higher protein and dry matter yields in Pennisetum americanum (pearl millet) and P. maximum”

✓ located verbatim in the source · abstract, characters 344–479 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Inoculation significantly increased protein and dry matter yields in guineagrass in 1975.

Taxon
P. maximumMegathyrsus maximuse0
Treatment
inoculatione0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
protein and dry matter yieldse0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Megathyrsus maximus
Megathyrsus maximus · TaxonHarry Rose from South West Rocks, Australia · CC BY 2.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“In 1975, inoculation produced significantly higher protein and dry matter yields in Pennisetum americanum (pearl millet) and P. maximum”

✓ located verbatim in the source · abstract, characters 344–479 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Observationobservedoriginal resultpositivesimulated

Claim · model-written summary

Regression projected inoculation would replace about 40 kg N ha-1 yr1 in pearl millet.

Taxon
pearl milletCenchrus americanuse0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
N ha-1 yr1 were replaced by inoculatione0
Direction
replacede0
Value
40 kg N ha-1 yr1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cenchrus americanus
Cenchrus americanus · TaxonPublic domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Projected yields using regression analysis of both pearl millet and guineagrass indicated that about 40 kg N ha-1 yr1 were replaced by inoculation”

✓ located verbatim in the source · abstract, characters 481–627 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Observationobservedoriginal resultpositivesimulated

Claim · model-written summary

Regression projected inoculation would replace about 40 kg N ha-1 yr1 in guineagrass.

Taxon
guineagrassMegathyrsus maximuse0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
N ha-1 yr1 were replaced by inoculatione0
Direction
replacede0
Value
40 kg N ha-1 yr1e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Megathyrsus maximus
Megathyrsus maximus · TaxonHarry Rose from South West Rocks, Australia · CC BY 2.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Projected yields using regression analysis of both pearl millet and guineagrass indicated that about 40 kg N ha-1 yr1 were replaced by inoculation”

✓ located verbatim in the source · abstract, characters 481–627 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Guineagrass inoculation yield responses in 1976 resembled 1975 despite lower overall production.

Taxon
guineagrassMegathyrsus maximuse0
Treatment
inoculatione0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
yield responsese0
Direction
similare0
Qualifier
overall production was lower during 1976e0
Comparator
those of 1975e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Megathyrsus maximus
Megathyrsus maximus · TaxonHarry Rose from South West Rocks, Australia · CC BY 2.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Although overall production was lower during 1976, yield responses due to inoculation were similar to those of 1975 in guineagrass”

✓ located verbatim in the source · abstract, characters 629–759 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nitrogen fertilizer enhanced inoculation response.

Taxon
grassese1
Treatment
Nitrogen fertilizere0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
response to inoculatione0
Direction
enhancee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Nitrogen fertilizer was required to enhance response to inoculation”

✓ located verbatim in the source · abstract, characters 761–828 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Higher nitrogen fertilizer rates repressed inoculation response.

Taxon
grassese1
Treatment
higher rates of nitrogen fertilizere0
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
responsee0
Direction
repressede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“higher rates of nitrogen fertilizer repressed response”

✓ located verbatim in the source · abstract, characters 833–887 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetylene reduction verified nitrogen fixation.

Taxon
grassese1
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
nitrogen fixation occurrede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Acetylene reduction verified that nitrogen fixation occurred”

✓ located verbatim in the source · abstract, characters 889–949 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Acetylene reduction did not correlate well with yield increases.

Taxon
grassese1
Interactor
Spirillum lipoferumAzospirillum brasilenset0symbiont
Outcome
yield increasese0
Direction
did not correlate welle0
Qualifier
Acetylene reductione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Acetylene reduction verified that nitrogen fixation occurred, but did not correlate well with yield increases”

✓ located verbatim in the source · abstract, characters 889–998 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Immunofluorescence identified and monitored Spirillum lipoferum populations in soil.

Taxon
grassese1
Interactor
Spirillum lipoferumAzospirillum brasilensee0symbiont
Outcome
identify and monitor Spirillum lipoferum popula tions in the soile0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Immunofluorescent techniques were used to specifically identify and monitor Spirillum lipoferum popula tions in the soil”

✓ located verbatim in the source · abstract, characters 1000–1120 · show in text ↑
e1

“grasses inoculated with tropical strains”

✓ located verbatim in the source · abstract, characters 66–106 · show in text ↑
t0

“Spirillum lipoferum”

✓ located verbatim in the source · title, characters 59–78 · show in text ↑

inoculation · Azospirillum brasilense · Cenchrus americanus (pearl millet)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

inoculation · Azospirillum brasilense · Megathyrsus maximus (Guinea grass)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.