Abstract
The aim of the present study was to investigate the importance of the preculture conditions including the vitrification solution on the survival of Rosa shoot tips cryopreserved using a combined vitrification-droplet procedure. In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation. Preculture was critical for the survival of rose shoot tips after cryopreservation. Among the different tested sugars and sugar alcohols (glucose, sucrose, mannitol and sorbitol) sucrose treatment for 48 h was the most efficient one. From the three vitrification solutions (PVS2, PVS3 and PVS4) tested, PVS2 ensured the highest survival rate. A one step vitrification protocol with PVS2 for 20 minutes was more efficient than a two step protocol that involved treatment of shoot tips with half strength PVS2 solution for 10 minutes after that with full strength PVS2 for an additional 15 minutes. After rapid rewarming and transfer of shoot tips on recovery medium, 66% survival for Fairy and 69% for wild roses were obtained. Plants produced from control and cryopreserved shoot tips were phenotypically similar.
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Claims 7
Claim · model-written summary
Sucrose pretreatment for 48 h was the most efficient sugar treatment before cryopreservation of rose shoot tips.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare0 - Treatment
- sucrose treatment for 48 he1
- Outcome
- the most efficient onee1
- Direction
- most efficiente1
- Comparator
- the different tested sugars and sugar alcoholse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e1“Among the different tested sugars and sugar alcohols (glucose, sucrose, mannitol and sorbitol) sucrose treatment for 48 h was the most efficient one”
Claim · model-written summary
PVS2 gave the highest survival rate among the three vitrification solutions tested for rose shoot tips.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- PVS2e3
- Outcome
- survival ratee3
- Direction
- higheste3
- Comparator
- PVS3 and PVS4e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e3“From the three vitrification solutions (PVS2, PVS3 and PVS4) tested, PVS2 ensured the highest survival rate”
Claim · model-written summary
A one-step vitrification protocol with PVS2 for 20 minutes was more efficient than a two-step protocol for rose shoot tips.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- one step vitrification protocol with PVS2 for 20 minutese4
- Outcome
- more efficiente4
- Direction
- more efficiente4
- Comparator
- a two step protocole4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e4“A one step vitrification protocol with PVS2 for 20 minutes was more efficient than a two step protocol”
Claim · model-written summary
Fairy rose shoot tips had 66% survival after rapid rewarming and transfer to recovery medium.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Material
- Fairye5
- Treatment
- After rapid rewarming and transfer of shoot tips on recovery mediume5
- Outcome
- survivale5
- Value
- 66%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e5“After rapid rewarming and transfer of shoot tips on recovery medium, 66% survival for Fairy and 69% for wild roses were obtained”
Claim · model-written summary
Wild rose shoot tips had 69% survival after rapid rewarming and transfer to recovery medium.
- Material
- wild rosese5
- Treatment
- After rapid rewarming and transfer of shoot tips on recovery mediume5
- Outcome
- survivale5
- Value
- 69%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e5“After rapid rewarming and transfer of shoot tips on recovery medium, 66% survival for Fairy and 69% for wild roses were obtained”
Claim · model-written summary
Plants regenerated from cryopreserved rose shoot tips were phenotypically similar to plants from control shoot tips.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- cryopreserved shoot tipse6
- Outcome
- phenotypically similare6
- Comparator
- controle6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e6“Plants produced from control and cryopreserved shoot tips were phenotypically similar”
Claim · model-written summary
Preculture was critical for the survival of rose shoot tips after cryopreservation.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- Preculturee2
- Outcome
- survivale2
- Qualifier
- criticale2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro grown roses (Rosa hybrida L.) cv. Fairy and wild roses (Rosa sp.) shoot tips were used for cryopreservation”
e2“Preculture was critical for the survival of rose shoot tips after cryopreservation”