Paper · 1989 · Annals of Botany

Control of Morphogenesis in Sorghum by 2,4-Dichlorophenoxyacetic Acid and Cytokinins

SHYAMALA BHASKARAN; ROBERTA H. SMITH

9
claims
16
verbatim quotes
30
citations
2
research areas
—
Not open access

Abstract

2, 4-Dichlorophenoxyacetic acid caused a shortening of roots and shoots when mature seeds of Sorghum bicolor (L.) Moench cv. BT×3197 were germinated on Murashige and Skoog (MS) medium that contained 2,4-D. Shoot growth was restored with cytokinins. A callus formed at the nodal region, the further differentiation of which was determined by the ratio of 2,4-D and cytokinins in the initial culture medium. A high auxin to cytokinin ratio promoted primarily root differentiation while a high cytokinin to auxin ratio promoted multiple bud development. Isolated shoot apical meristem with the subtending node produced embryogenic callus at low cytokinin levels and green buds on high cytokinin levels when cultured in the presence of 2,4-D. It is concluded that cells potentially capable of differentiation into roots, somatic embryos or axillary buds are present in the first nodal region.

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Claims 9

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

2,4-D shortened roots of germinated sorghum seeds.

Taxon
Sorghum bicolorSorghum bicolore0
Material
BT×3197e0
Treatment
2, 4-Dichlorophenoxyacetic acide0
Outcome
rootse0
Direction
shorteninge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“2, 4-Dichlorophenoxyacetic acid caused a shortening of roots and shoots when mature seeds of Sorghum bicolor (L.) Moench cv. BT×3197 were germinated on Murashige and Skoog (MS) medium that contained 2,4-D”

✓ located verbatim in the source · abstract, characters 0–204 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

2,4-D shortened shoots of germinated sorghum seeds.

Taxon
Sorghum bicolorSorghum bicolore0
Material
BT×3197e0
Treatment
2, 4-Dichlorophenoxyacetic acide0
Outcome
shootse0
Direction
shorteninge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum bicolor
Sorghum bicolor · TaxonNo machine-readable author provided. Pethan assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“2, 4-Dichlorophenoxyacetic acid caused a shortening of roots and shoots when mature seeds of Sorghum bicolor (L.) Moench cv. BT×3197 were germinated on Murashige and Skoog (MS) medium that contained 2,4-D”

✓ located verbatim in the source · abstract, characters 0–204 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cytokinins restored shoot growth.

Taxon
SorghumSorghumt0
Treatment
cytokininse0
Outcome
Shoot growthe0
Direction
restorede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Shoot growth was restored with cytokinins”

✓ located verbatim in the source · abstract, characters 206–247 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus formed at the sorghum nodal region.

Taxon
SorghumSorghumt0
Organ / stage
nodal regione0
Outcome
A callus formede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“A callus formed at the nodal region”

✓ located verbatim in the source · abstract, characters 249–284 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A high auxin to cytokinin ratio primarily promoted root differentiation.

Taxon
SorghumSorghumt0
Treatment
high auxin to cytokinin ratioe0
Outcome
root differentiatione0
Direction
promotede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“A high auxin to cytokinin ratio promoted primarily root differentiation”

✓ located verbatim in the source · abstract, characters 406–477 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A high cytokinin to auxin ratio promoted multiple bud development.

Taxon
SorghumSorghumt0
Treatment
high cytokinin to auxin ratioe0
Outcome
multiple bud developmente0
Direction
promotede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“a high cytokinin to auxin ratio promoted multiple bud development”

✓ located verbatim in the source · abstract, characters 484–549 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Low cytokinin levels yielded embryogenic callus from shoot apical meristems with nodes in 2,4-D.

Taxon
SorghumSorghumt0
Organ / stage
shoot apical meristem with the subtending nodee0
Treatment
low cytokinin levelse0
Outcome
embryogenic calluse0
Comparator
high cytokinin levelse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Isolated shoot apical meristem with the subtending node produced embryogenic callus at low cytokinin levels and green buds on high cytokinin levels when cultured in the presence of 2,4-D”

✓ located verbatim in the source · abstract, characters 551–737 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

High cytokinin levels yielded green buds from shoot apical meristems with nodes in 2,4-D.

Taxon
SorghumSorghumt0
Organ / stage
shoot apical meristem with the subtending nodee0
Treatment
high cytokinin levelse0
Outcome
green budse0
Comparator
low cytokinin levelse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Isolated shoot apical meristem with the subtending node produced embryogenic callus at low cytokinin levels and green buds on high cytokinin levels when cultured in the presence of 2,4-D”

✓ located verbatim in the source · abstract, characters 551–737 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The first nodal region was inferred to contain cells capable of root, embryo or axillary bud differentiation.

Taxon
SorghumSorghumt0
Organ / stage
first nodal regione0
Outcome
cells potentially capable of differentiation into roots, somatic embryos or axillary budse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“cells potentially capable of differentiation into roots, somatic embryos or axillary buds are present in the first nodal region”

✓ located verbatim in the source · abstract, characters 760–887 · show in text ↑
t0

“Sorghum”

✓ located verbatim in the source · title, characters 28–35 · show in text ↑

class · growth · SorghumResults agree

11 papers: 11 positive, 0 negative or no effect

10 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

7 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.