Paper · 1986 · HortScience

Thidiazuron Stimulation of Apple Shoot Proliferation in Vitro

J. P. van Nieuwkerk; Richard H. Zimmerman; Ingrid Fordham

6
claims
11
verbatim quotes
142
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Abstract Thidiazuron stimulated shoot proliferation on shoot tip explants of ‘Gala’ apple ( Malus domestica Borkh.) when incorporated in Linsmaier-Skoog medium at concentrations of 10, 1, and 0.1 μ m . Shoot numbers with thidiazuron were equivalent to, or greater than, the number produced when using 4.4 μ m BA in the medium, but the shoots were shorter than with BA. At 10 and 1 μ m thidiazuron, shoot leaves were narrow, had pointed laminae, and were somewhat distorted. Shoot proliferation continued when explants from the 2 highest thidiazuron concentrations were transferred to cytokinin-free medium. Shoots from cultures grown at all 3 concentrations of thidiazuron rooted after 1 to 2 subcultures on cytokinin-free medium. Chemical names used: N -(phenylmethyl)-l H -purin-6-amine (BA) and N -phenyl- N ′-l,2,3-thiadiazol-5-ylurea (thidiazuron).

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thidiazuron at three concentrations stimulated shoot proliferation on Gala apple shoot tips.

Material
‘Gala’e0
Organ / stage
shoot tip explantse0
Treatment
Thidiazurone0
Outcome
shoot proliferatione0
Direction
stimulatede0
Qualifier
concentrations of 10, 1, and 0.1 μ me0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Thidiazuron stimulated shoot proliferation on shoot tip explants of ‘Gala’ apple ( Malus domestica Borkh.) when incorporated in Linsmaier-Skoog medium at concentrations of 10, 1, and 0.1 μ m .”

✓ located verbatim in the source · abstract, characters 9–201 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thidiazuron produced shoot numbers equivalent to or greater than BA.

Treatment
thidiazurone0
Outcome
Shoot numberse0
Direction
equivalent to, or greater thane0
Comparator
4.4 μ m BAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Shoot numbers with thidiazuron were equivalent to, or greater than, the number produced when using 4.4 μ m BA in the medium, but the shoots were shorter than with BA.”

✓ located verbatim in the source · abstract, characters 202–368 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 27–32 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Thidiazuron produced shorter apple shoots than BA.

Treatment
thidiazurone0
Outcome
shootse0
Direction
shortere0
Comparator
BAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Shoot numbers with thidiazuron were equivalent to, or greater than, the number produced when using 4.4 μ m BA in the medium, but the shoots were shorter than with BA.”

✓ located verbatim in the source · abstract, characters 202–368 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 27–32 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

At 10 and 1 μ m thidiazuron, apple leaves were narrow, pointed, and distorted.

Organ / stage
shoot leavese0
Treatment
10 and 1 μ m thidiazurone0
Outcome
narrow, had pointed laminae, and were somewhat distortede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“At 10 and 1 μ m thidiazuron, shoot leaves were narrow, had pointed laminae, and were somewhat distorted.”

✓ located verbatim in the source · abstract, characters 369–473 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 27–32 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoot proliferation persisted after explants from the two highest thidiazuron concentrations moved to cytokinin-free medium.

Treatment
cytokinin-free mediume0
Outcome
Shoot proliferation continuede0
Qualifier
2 highest thidiazuron concentrationse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Shoot proliferation continued when explants from the 2 highest thidiazuron concentrations were transferred to cytokinin-free medium.”

✓ located verbatim in the source · abstract, characters 474–606 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 27–32 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoots from all three thidiazuron concentrations rooted after one to two subcultures on cytokinin-free medium.

Treatment
cytokinin-free mediume0
Outcome
rootede0
Qualifier
after 1 to 2 subculturese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Shoots from cultures grown at all 3 concentrations of thidiazuron rooted after 1 to 2 subcultures on cytokinin-free medium.”

✓ located verbatim in the source · abstract, characters 607–730 · show in text ↑
t0

“Apple”

✓ located verbatim in the source · title, characters 27–32 · show in text ↑

Thidiazuron · growth · Malus domestica (apple)Positive and negative results

10 papers: 7 positive, 3 negative or no effect

6 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.