Abstract
An endophytic bacterial strain was isolated from kimchi, a Korean traditional fermented Brassica campestris and identified as Bacillus subtilis MJMP2 based on the 16S rRNA sequence. This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease, as well as activity against some other rice phytopathogenic fungi. The active compound was purified through size-exclusion chromatography and preparative High-performance liquid chromatography. The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A. Furthermore, a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay. The crude extract also enhanced the length of roots of Arabidopsis thaliana. These results suggest that the strain Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Bacillus subtilis MJMP2 antagonized Xanthomonas oryzae pv. oryzae.
- Taxon
- rice
Oryza sativae1 - Interactor
- Xanthomonas oryzae pv. oryzae (Xoo) KACC10331
Xanthomonas oryzae pv. oryzaee1pathogen - Second interactor
- Bacillus subtilisMJMP2
Bacillus subtilist0biocontrol_agent - Outcome
- antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilisMJMP2 from Kimchi inhibitsXanthomonas oryzaepv.oryzae, the pathogen of Rice bacterial blight disease”
e1“This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease”
Claim · model-written summary
The strain also acted against other rice pathogenic fungi.
- Taxon
- riceOryza sativae2
- Interactor
- rice phytopathogenic fungie2pathogen
- Outcome
- activity against some other rice phytopathogenic fungie2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice bacterial blight disease”
e2“activity against some other rice phytopathogenic fungi”
Claim · model-written summary
The purified active compound had m/z 1043, identical to iturin A.
- Taxon
- riceOryza sativae3
- Outcome
- molecular weighte4
- Value
- m/z 1043e4
- Qualifier
- identical to that of iturin Ae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“rice leaf explant assay”
e4“The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A.”
Claim · model-written summary
MJMP2 crude extract inhibited rice blight in a leaf explant assay.
- Taxon
- riceOryza sativae5
- Organ / stage
- rice leafe5
- Treatment
- a crude extract from the culture supernatant of Bacillus subtilis MJMP2e5
- Interactor
- Xanthomonas oryzaepv.oryzaet0pathogen
- Outcome
- rice blight diseasee5
- Direction
- inhibitorye5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Xanthomonas oryzaepv.oryzae”
e5“a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay”
Claim · model-written summary
MJMP2 crude extract inhibited rice blight in a pot assay.
- Taxon
- riceOryza sativae6
- Treatment
- a crude extract from the culture supernatant of Bacillus subtilis MJMP2e6
- Outcome
- rice blight diseasee6
- Direction
- inhibitorye6
- Setting
- pot assaye6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay”
Claim · model-written summary
The crude extract increased Arabidopsis root length.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae7 - Organ / stage
- rootse7
- Treatment
- crude extracte7
- Outcome
- length of rootse7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The crude extract also enhanced the length of roots of Arabidopsis thaliana.”
Claim · model-written summary
The authors suggest MJMP2 for rice blight biological control.
- Taxon
- riceOryza sativae0
- Outcome
- Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight diseasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease”
The same questions in other papers
Bacillus subtilis · disease · Oryza sativa (rice)Positive and negative results
13 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
11 papers more in this group
See all 15 papers →botanical · disease · Oryza sativa (rice)Positive and negative results
73 papers in the same direction as this paper · 8 in the opposite direction
- negative
- negative
- negative
78 papers more in this group
See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.