Abstract
Infection of root lession nematode, Pratylenchus coffeae, becomes an important factor causing yield loss in coffee production in Indonesia. Biological control of P. coffeae needs to be developed to meet the requirement of environmentally save crop production. The research was conducted to evaluate 3 selected endophytic bacteria isolates, Ochrobactrum intermedium DSQ5, Klebsiella oxytoca NGB-FR 50, and Bacillus subtilis AIMST 10.T18.1, from coffee in controlling P. coffeae. Research methods involved pathogenicity test based on hypersensitive reaction and haemolysis test, and evaluation of their ability to suppress P. coffeae in vitro and in planta. The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human. In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%. Those results correlated positively with in planta assay’s result.
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Claims 4
Claim · model-written summary
In vitro, K. oxytoca isolate C939A32 suppressed Pratylenchus coffeae populations by 66.7%.
- Treatment
- K. oxytoca - C939A32e1
- Interactor
- P. coffeae
Pratylenchus coffeaee1pest - Second interactor
- K. oxytoca - C939A32
Klebsiella oxytocae1biocontrol_agent - Outcome
- P. coffeae populationse1
- Direction
- suppresse1
- Value
- 66.7%e1
- Setting
- In vitroe1
CoffeaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Isolated from Coffee Plant”
e1“In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%”
Claim · model-written summary
In vitro, O. intermedium isolate C939A3 suppressed Pratylenchus coffeae populations by 100%.
- Treatment
- O. intermedium - C939A3e1
- Interactor
- P. coffeaePratylenchus coffeaee1pest
- Second interactor
- O. intermedium - C939A3Brucella intermediae1biocontrol_agent
- Outcome
- P. coffeae populationse1
- Direction
- suppresse1
- Value
- 100%e1
- Setting
- In vitroe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Isolated from Coffee Plant”
e1“In vitro assays showed that two isolates, K. oxytoca - C939A32 and O. intermedium - C939A3, could suppress P. coffeae populations by 66.7% and 100%”
Claim · model-written summary
In vitro suppression results correlated positively with the in planta results.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Isolated from Coffee Plant”
e2“Those results correlated positively with in planta”
Claim · model-written summary
The three endophytic isolates gave negative hypersensitive and haemolysis reactions, so they were not pathogenic to plant or human.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Isolated from Coffee Plant”
e3“The hypersensitive and haemolysis reactions indicated that these 3 isolates showed negative result both to plant and human”