Abstract
Triterpenoids are the principal effective components of the traditional medicine 'Sanqi'(Panax notoginseng).They are synthesized via the isoprene pathway.The elucidation and alteration of isoprene pathway would be beneficial for the regulation of triterpenoid biosynthesis in plants.Farnesyl pyrophosphate synthase(FPS) is one of the key enzymes for the pathway.A cDNA encoding FPS was cloned from P.notoginseng root by RT-PCR,3′-RACE and 5′-terminal sequence analysis.Some physicochemical properties of P.notoginseng FPS were revealed by biological information resources and correlation analysis with use of EBI Clustal W and the Protein Information Resource search/analysis tool.Multiple alignment of plant FPS resulted in the construction of a phylogenetic tree of plant FPS registered in GenBank through the neighbor-joining method.The complete cDNA sequence of isolated P.notoginseng FPS was submitted to GenBank(accession no.DQ059550).The FPS contains two conservative core fragments,DDIMD and QVQDDYLD,and shows 99% and 95% sequence identity to Panax ginseng and Centella asiatica,respectively,with the triterpenoid structures among the above plants being very similar.
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Claims 5
Claim · model-written summary
A cDNA encoding FPS was cloned from Panax notoginseng root.
- Taxon
- Panax notoginseng
Panax notoginsengt0 - Organ / stage
- roote0
- Gene
- FPSe0
- Outcome
- cDNA encoding FPSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Comparison Analysis of FPS from Panax notoginseng with Other Plant FPS Registered in GenBank”
e0“A cDNA encoding FPS was cloned from P.notoginseng root by RT-PCR,3′-RACE and 5′-terminal sequence analysis”
Claim · model-written summary
The P. notoginseng FPS contains two conserved core fragments, DDIMD and QVQDDYLD.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Gene
- FPSe1
- Outcome
- conservative core fragmentse1
- Value
- DDIMD and QVQDDYLDe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Comparison Analysis of FPS from Panax notoginseng with Other Plant FPS Registered in GenBank”
e1“The FPS contains two conservative core fragments,DDIMD and QVQDDYLD”
Claim · model-written summary
P. notoginseng FPS shows 99% sequence identity to Panax ginseng.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Gene
- FPSt0
- Outcome
- sequence identitye2
- Value
- 99%e2
- Comparator
- Panax ginsenge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Comparison Analysis of FPS from Panax notoginseng with Other Plant FPS Registered in GenBank”
e2“shows 99% and 95% sequence identity to Panax ginseng and Centella asiatica”
Claim · model-written summary
P. notoginseng FPS shows 95% sequence identity to Centella asiatica.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Gene
- FPSt0
- Outcome
- sequence identitye2
- Value
- 95%e2
- Comparator
- Centella asiaticae2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Comparison Analysis of FPS from Panax notoginseng with Other Plant FPS Registered in GenBank”
e2“shows 99% and 95% sequence identity to Panax ginseng and Centella asiatica”
Claim · model-written summary
The triterpenoid structures of the compared plants are very similar.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- triterpenoid structurese3
- Direction
- very similare3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Comparison Analysis of FPS from Panax notoginseng with Other Plant FPS Registered in GenBank”
e3“with the triterpenoid structures among the above plants being very similar”