Abstract
In this study,we optimized main factors(genotypes,explants,etc) affecting soybean Agrobacterium-mediated transformation system by comparing GUS transient expression rate with embryonic tip,cotyledonary node and hypocotyl of Zhechun No. 3,Huachun No. 6 and 0428. The results showed GUS transient expression rate of the three soybean genotypes was low in the embryonic tip transformation system and tufty shoots showed no expression. Using the hypocotyl transformation system,the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 43. 92%,54. 45%,and 58. 47%,respectively,after 5 days of germination and 3 co-culture days. Using the cotyledonary node transformation system( 5-day germination),the optimal co-culture time for Zhechun No. 3,Huachun No. 6 and 0428 were 5,4,and 4 days respectively and the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 23. 05%,13. 79%,and 4. 78%,respectively. The germination time of cotyledonary node transformation system was further optimized using Zhechun No. 3. The results showed that both gene expression and shoot induction increased dramatically in 3 germination days compared to 5 germination days.
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Claims 13
Claim · model-written summary
Embryonic tip transformation gave low GUS transient expression across three soybean genotypes.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- embryonic tipe0
- Outcome
- GUS transient expression ratee0
- Direction
- lowe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS transient expression rate of the three soybean genotypes was low in the embryonic tip transformation system”
Claim · model-written summary
Tufty shoots in the embryonic tip system showed no expression.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- tufty shootse0
- Outcome
- no expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tufty shoots showed no expression”
t0“soybean”
Claim · model-written summary
Hypocotyl transformation of Zhechun No. 3 reached a maximum tufty shoot expression rate of 43. 92%.
- Taxon
- soybeanGlycine maxt0
- Material
- Zhechun No. 3e0
- Organ / stage
- tufty shootse0
- Outcome
- expression ratee0
- Direction
- maximume0
- Value
- 43. 92%e0
- Qualifier
- after 5 days of germination and 3 co-culture dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using the hypocotyl transformation system,the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 43. 92%,54. 45%,and 58. 47%,respectively,after 5 days of germination and 3 co-culture days”
t0“soybean”
Claim · model-written summary
Hypocotyl transformation of Huachun No. 6 reached a maximum tufty shoot expression rate of 54. 45%.
- Taxon
- soybeanGlycine maxt0
- Material
- Huachun No. 6e0
- Organ / stage
- tufty shootse0
- Outcome
- expression ratee0
- Direction
- maximume0
- Value
- 54. 45%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using the hypocotyl transformation system,the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 43. 92%,54. 45%,and 58. 47%,respectively,after 5 days of germination and 3 co-culture days”
t0“soybean”
Claim · model-written summary
Hypocotyl transformation of 0428 reached a maximum tufty shoot expression rate of 58. 47%.
- Taxon
- soybeanGlycine maxt0
- Material
- 0428e0
- Organ / stage
- tufty shootse0
- Outcome
- expression ratee0
- Direction
- maximume0
- Value
- 58. 47%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using the hypocotyl transformation system,the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 43. 92%,54. 45%,and 58. 47%,respectively,after 5 days of germination and 3 co-culture days”
t0“soybean”
Claim · model-written summary
In cotyledonary node transformation, Zhechun No. 3 had an optimal co-culture time of 5 days.
- Taxon
- soybeanGlycine maxt0
- Material
- Zhechun No. 3e0
- Outcome
- optimal co-culture timee0
- Value
- 5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimal co-culture time for Zhechun No. 3,Huachun No. 6 and 0428 were 5,4,and 4 days respectively”
t0“soybean”
Claim · model-written summary
In cotyledonary node transformation, Huachun No. 6 had an optimal co-culture time of 4 days.
- Taxon
- soybeanGlycine maxt0
- Material
- Huachun No. 6e0
- Outcome
- optimal co-culture timee0
- Value
- 4 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimal co-culture time for Zhechun No. 3,Huachun No. 6 and 0428 were 5,4,and 4 days respectively”
t0“soybean”
Claim · model-written summary
In cotyledonary node transformation, 0428 had an optimal co-culture time of 4 days.
- Taxon
- soybeanGlycine maxt0
- Material
- 0428e0
- Outcome
- optimal co-culture timee0
- Value
- 4 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimal co-culture time for Zhechun No. 3,Huachun No. 6 and 0428 were 5,4,and 4 days respectively”
t0“soybean”
Claim · model-written summary
Cotyledonary node transformation gave maximum tufty shoot expression of 23. 05% for Zhechun No. 3.
- Taxon
- soybeanGlycine maxt0
- Material
- Zhechun No. 3e0
- Outcome
- expression ratee0
- Direction
- maximume0
- Value
- 23. 05%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 23. 05%,13. 79%,and 4. 78%,respectively”
t0“soybean”
Claim · model-written summary
Cotyledonary node transformation gave maximum tufty shoot expression of 13. 79% for Huachun No. 6.
- Taxon
- soybeanGlycine maxt0
- Material
- Huachun No. 6e0
- Outcome
- expression ratee0
- Direction
- maximume0
- Value
- 13. 79%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 23. 05%,13. 79%,and 4. 78%,respectively”
t0“soybean”
Claim · model-written summary
Cotyledonary node transformation gave maximum tufty shoot expression of 4. 78% for 0428.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum expression rate of Zhechun No. 3,Huachun No. 6 and 0428 in tufty shoots was 23. 05%,13. 79%,and 4. 78%,respectively”
t0“soybean”
Claim · model-written summary
In Zhechun No. 3, three germination days increased gene expression compared with five days.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 3 germination dayse0
- Outcome
- gene expressione0
- Direction
- increasede0
- Comparator
- 5 germination dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both gene expression and shoot induction increased dramatically in 3 germination days compared to 5 germination days”
t0“soybean”
Claim · model-written summary
In Zhechun No. 3, three germination days increased shoot induction compared with five days.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 3 germination dayse0
- Outcome
- shoot inductione0
- Direction
- increasede0
- Comparator
- 5 germination dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both gene expression and shoot induction increased dramatically in 3 germination days compared to 5 germination days”
t0“soybean”
The same questions in other papers
processing · growth · Glycine max (soybean)Positive and negative results
29 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
39 papers more in this group
See all 43 papers →processing · molecular: expression · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.