Abstract
Autocatalytic C 2 H 4 biosynthesis in preclimacteric apple fruit ( Malus domestica Borkh. `Golden Delicious') was prevented by storage in atmospheres of 20% CO 2 -enriched air (17% O 2 + 63% N 2 ) or 0.25% O 2 (balance N 2 ). In preclimacteric fruit, both treatments inhibited C 2 H 2 biosynthesis by suppressing expression of ACC synthase (ACC-S) at the mRNA level. ACC oxidase (ACC-O) mRNA abundance and in vitro enzyme activity also were impaired by these treatments. However, the conversion of ACC to C 2 H 4 never became the rate limiting step in C 2 H 4 biosynthesis. C 2 H 4 biosynthesis also was effectively inhibited in climacteric apple fruit kept in air + 20% CO 2 or 0.25% O 2 . Climacteric apples also exhibited suppressed expression of ACC-S at the mRNA level, while ACC-O transcript abundance, enzyme activity, and protein abundance were reduced only slightly. ACC-S is the key regulatory enzyme of C 2 H 4 biosynthesis and is the major site at which elevated CO 2 and reduced O 2 atmospheres inhibit C 2 H 4 biosynthesis, irrespective of fruit physiological maturity. Chemical names used: 1-aminocyclopropane-1-carboxcylic acid (ACC).
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Claims 10
Claim · model-written summary
Carbon dioxide enriched air prevented autocatalytic ethylene biosynthesis in preclimacteric apple fruit.
- Taxon
- apple
Malus domesticae0 - Material
- Golden Deliciouse0
- Organ / stage
- preclimacteric apple fruite0
- Treatment
- 20% CO 2 -enriched aire0
- Outcome
- C 2 H 4 biosynthesise0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Autocatalytic C 2 H 4 biosynthesis in preclimacteric apple fruit ( Malus domestica Borkh. `Golden Delicious') was prevented by storage in atmospheres of 20% CO 2 -enriched air (17% O 2 + 63% N 2 ) or 0.25% O 2 (balance N 2 )”
Claim · model-written summary
Low oxygen prevented autocatalytic ethylene biosynthesis in preclimacteric apple fruit.
- Taxon
- appleMalus domesticae0
- Material
- Golden Deliciouse0
- Organ / stage
- preclimacteric apple fruite0
- Treatment
- 0.25% O 2e0
- Outcome
- C 2 H 4 biosynthesise0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Autocatalytic C 2 H 4 biosynthesis in preclimacteric apple fruit ( Malus domestica Borkh. `Golden Delicious') was prevented by storage in atmospheres of 20% CO 2 -enriched air (17% O 2 + 63% N 2 ) or 0.25% O 2 (balance N 2 )”
Claim · model-written summary
Both storage treatments suppressed ACC synthase expression in preclimacteric apple fruit.
- Taxon
- AppleMalus domesticat0
- Organ / stage
- preclimacteric fruite0
- Treatment
- both treatmentse0
- Gene
- ACC synthase (ACC-S)e0
- Outcome
- expression of ACC synthase (ACC-S) at the mRNA levele0
- Direction
- suppressinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In preclimacteric fruit, both treatments inhibited C 2 H 2 biosynthesis by suppressing expression of ACC synthase (ACC-S) at the mRNA level”
t0“Low Oxygen and Elevated Carbon Dioxide Atmospheres Inhibit Ethylene Biosynthesis in Preclimacteric and Climacteric Apple Fruit”
Claim · model-written summary
Both treatments impaired ACC oxidase mRNA abundance and in vitro activity.
- Taxon
- AppleMalus domesticat0
- Treatment
- these treatmentse0
- Gene
- ACC oxidase (ACC-O)e0
- Outcome
- ACC oxidase (ACC-O) mRNA abundance and in vitro enzyme activitye0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC oxidase (ACC-O) mRNA abundance and in vitro enzyme activity also were impaired by these treatments”
t0“Low Oxygen and Elevated Carbon Dioxide Atmospheres Inhibit Ethylene Biosynthesis in Preclimacteric and Climacteric Apple Fruit”
Claim · model-written summary
ACC conversion did not become rate limiting in ethylene biosynthesis.
- Taxon
- AppleMalus domesticat0
- Outcome
- conversion of ACC to C 2 H 4 never became the rate limiting stepe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the conversion of ACC to C 2 H 4 never became the rate limiting step in C 2 H 4 biosynthesis”
t0“Low Oxygen and Elevated Carbon Dioxide Atmospheres Inhibit Ethylene Biosynthesis in Preclimacteric and Climacteric Apple Fruit”
Claim · model-written summary
High carbon dioxide inhibited ethylene biosynthesis in climacteric fruit.
- Taxon
- appleMalus domesticae0
- Organ / stage
- climacteric apple fruite0
- Treatment
- air + 20% CO 2e0
- Outcome
- C 2 H 4 biosynthesise0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C 2 H 4 biosynthesis also was effectively inhibited in climacteric apple fruit kept in air + 20% CO 2 or 0.25% O 2”
Claim · model-written summary
Low oxygen inhibited ethylene biosynthesis in climacteric fruit.
- Taxon
- appleMalus domesticae0
- Organ / stage
- climacteric apple fruite0
- Treatment
- 0.25% O 2e0
- Outcome
- C 2 H 4 biosynthesise0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C 2 H 4 biosynthesis also was effectively inhibited in climacteric apple fruit kept in air + 20% CO 2 or 0.25% O 2”
Claim · model-written summary
Climacteric apples showed suppressed ACC synthase mRNA expression.
- Taxon
- applesMalus domesticae0
- Gene
- ACC-Se0
- Outcome
- expression of ACC-S at the mRNA levele0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Climacteric apples also exhibited suppressed expression of ACC-S at the mRNA level”
Claim · model-written summary
Climacteric apples showed only slight reductions in ACC oxidase transcript, activity and protein.
- Taxon
- AppleMalus domesticat0
- Gene
- ACC-Oe0
- Outcome
- ACC-O transcript abundance, enzyme activity, and protein abundancee0
- Direction
- reducede0
- Qualifier
- only slightlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC-O transcript abundance, enzyme activity, and protein abundance were reduced only slightly”
t0“Low Oxygen and Elevated Carbon Dioxide Atmospheres Inhibit Ethylene Biosynthesis in Preclimacteric and Climacteric Apple Fruit”
Claim · model-written summary
ACC synthase was identified as the major regulatory site of atmosphere-mediated inhibition.
- Taxon
- AppleMalus domesticat0
- Treatment
- elevated CO 2 and reduced O 2 atmospherese0
- Gene
- ACC-Se0
- Outcome
- C 2 H 4 biosynthesise0
- Direction
- inhibite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC-S is the key regulatory enzyme of C 2 H 4 biosynthesis and is the major site at which elevated CO 2 and reduced O 2 atmospheres inhibit C 2 H 4 biosynthesis”
t0“Low Oxygen and Elevated Carbon Dioxide Atmospheres Inhibit Ethylene Biosynthesis in Preclimacteric and Climacteric Apple Fruit”
The same questions in other papers
co2 · molecular: metabolites · Malus domestica (apple)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.