Abstract
To improve phytoalexin synthesis capability of hairy roots,farnesyl diphosphate synthase gene(fps) of Mentha spicata L.was transferred into tobacco via Ri-mediated transformation.The engineering Agrobacterium rhizogenes strain Ar1334 harboured the binary vector pBinARfps,in which the fps cDNA was placed under 35S promoter.The leaf explants of K326 co-cultured with Ar1334 were transformed on MS medium to induce hairy roots.Among the 18 lines of hairy root induced,4 lines showed some kanamycin(20 mg·L1) resistance.All the transgenic hairy root lines showed typical hairy root characteristics on MS medium without any plant hormone.Polymerase chain reaction and Southern blot revealed the foreign gene fps was intergrated into these 4 lines.Northern blot analysis indicated that the foreign fps gene was expressed at the transcriptional level.Transgenic hairy root lines showed higher FPS activity than the control and disease challenge also proved that the juice of transgenic hairy roots could inhibit the growth of Alternaria alternata.This result proved that foreign fps gene expression could improve the accumulation of resistance substance in hairy roots in vitro culture.
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Claims 7
Claim · model-written summary
Of 18 induced hairy root lines, 4 showed some kanamycin resistance.
Verbatim
t0“Nicotinan tabacum”
e0“Among the 18 lines of hairy root induced,4 lines showed some kanamycin(20 mg·L1) resistance”
Claim · model-written summary
All transgenic hairy root lines showed typical hairy root characteristics on hormone-free MS medium.
Verbatim
t0“Nicotinan tabacum”
e0“All the transgenic hairy root lines showed typical hairy root characteristics on MS medium without any plant hormone”
Claim · model-written summary
PCR and Southern blot showed the foreign fps gene was integrated into the 4 lines.
Verbatim
t0“Nicotinan tabacum”
e0“Polymerase chain reaction and Southern blot revealed the foreign gene fps was intergrated into these 4 lines”
Claim · model-written summary
Northern blot showed the foreign fps gene was expressed at the transcriptional level.
Verbatim
t0“Nicotinan tabacum”
e0“Northern blot analysis indicated that the foreign fps gene was expressed at the transcriptional level”
Claim · model-written summary
Transgenic hairy root lines showed higher FPS activity than the control.
Verbatim
t0“Nicotinan tabacum”
e0“Transgenic hairy root lines showed higher FPS activity than the control”
Claim · model-written summary
Juice of transgenic hairy roots inhibited the growth of Alternaria alternata.
- Taxon
- Nicotinan tabacumt0
- Treatment
- juice of transgenic hairy rootse0
- Interactor
- Alternaria alternata
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Alternaria alternatae0pathogen - Outcome
- growth of Alternaria alternatae0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Nicotinan tabacum”
e0“the juice of transgenic hairy roots could inhibit the growth of Alternaria alternata”
Claim · model-written summary
The authors conclude that fps expression could improve accumulation of resistance substance in hairy roots in vitro.
Verbatim
t0“Nicotinan tabacum”
e0“foreign fps gene expression could improve the accumulation of resistance substance in hairy roots in vitro culture”