Abstract
The maize stalk of the third internode in the maturity stage is used as the research material.The research uses four ways,such as free-hand sectioning,frozen sectioning,paraffin sectioning,thin sectioning and compares the quality of the slice of different methods.This research lays the technical foundation for studying the relationship between the structure of maize stalks and lodging.The results show that freehand sectioning method for getting the structure slice of mature maize stalks is a relatively convenient and rapid method,and the area of slice is relatively big.The slice is suitable for a wide range of observation and statistics.Frozen sectioning is a fast way to get the slice of mature maize stalks,and the area of slice is relatively small and the slice is suitable for a small range of observation.Thin sectioning is a good way to get perfect slice.It fits for high multiple microscope observation and small range of SEM observation to observe tissue structures,but the slice area is too small.Paraffin sectioning is not suitable for getting the structure slice of mature maize stalks.The research suggests that free-hand sectioning method is the most suitable way for observation and study of the structure of mature maize stalks.
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Claims 6
Claim · model-written summary
Freehand sectioning produced mature maize stalk slices conveniently and rapidly with relatively large area.
- Organ / stage
- mature maize stalkse0
- Outcome
- relatively convenient and rapid method,and the area of slice is relatively bige0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“freehand sectioning method for getting the structure slice of mature maize stalks is a relatively convenient and rapid method,and the area of slice is relatively big”
Claim · model-written summary
Freehand slices were suitable for broad observation and statistics.
- Outcome
- suitable for a wide range of observation and statisticse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The slice is suitable for a wide range of observation and statistics”
t0“Maize Stalks”
Claim · model-written summary
Frozen sectioning was fast but gave relatively small slices for small range observation.
- Outcome
- area of slice is relatively small and the slice is suitable for a small range of observatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Frozen sectioning is a fast way to get the slice of mature maize stalks,and the area of slice is relatively small and the slice is suitable for a small range of observation”
Claim · model-written summary
Thin sectioning produced a good slice for high magnification microscopy and small range SEM observation, but its area was too small.
- Outcome
- perfect slicee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thin sectioning is a good way to get perfect slice.It fits for high multiple microscope observation and small range of SEM observation to observe tissue structures,but the slice area is too small”
t0“Maize Stalks”
Claim · model-written summary
Paraffin sectioning was unsuitable for mature maize stalk structural slices.
- Outcome
- not suitable for getting the structure slicee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Paraffin sectioning is not suitable for getting the structure slice of mature maize stalks”
Claim · model-written summary
Freehand sectioning was recommended for observing mature maize stalk structure.
- Outcome
- free-hand sectioning method is the most suitable way for observation and study of the structuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free-hand sectioning method is the most suitable way for observation and study of the structure of mature maize stalks”