Abstract
In order to examine the feasibility of using winter buds of special mulberry germplasm resources to regenerate plantlets and screen suitable growth regulators and appropriate contents in culture medium,winter buds of different special mulberry germplasm resources were cultured on media containing various kinds and amounts of growth regulators and were induced to regenerate mulberry plantlet.The results showed that the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators.Growth hormone TDZ supplemented in the media could induce callus formation and growth but inhibited bud growth.Cytokinin 6-BA supplemented in the media promoted bud regeneration and the rate of winter bud regeneration on medium containing 3.0 mg/L 6-BA was significantly higher than that on medium containing 1.0 mg/L 6-BA.Among the 8 mulberry germplasm resources whose winter buds were cultured on media containing 3.0 mg/L 6-BA,Xinjiang Yaosang and Xiaohuaye Pisang had the highest inducing rate of clustered buds with winter bud regeneration rate up to 66.7%.Diansang took the second place with winter bud regeneration rate of 55.5%.Tianmushan Sang and Sri Lanka had winter bud regeneration rate of 40.0% and 25.0% respectively.One month after winter-bud-regenerated shoots of 5 mulberry germplasm resources were transferred onto rooting medium,only shoots of Xinjiang Yaosang and Diansang formed root system(the induction rate was 20.0% and 14.3% respectively) and survived after being transplanted into soil.These results preliminarily showed that in vitro tissue culture and plant regeneration is an effective method to conserve and utilize rare and endangered mulberry germplasm resources such as Diansang and Xinjiang Yaosang(a natural 22-ploid species).
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Claims 15
Claim · model-written summary
Winter buds of tested mulberry germplasm stopped growing on media without growth regulators.
- Taxon
- mulberrye1
- Organ / stage
- winter budse1
- Treatment
- media not supplemented with growth regulatorse1
- Outcome
- growinge1
- Direction
- stoppede1
Not reportedComparator
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
Claim · model-written summary
TDZ in the medium could induce callus formation and growth of mulberry winter buds.
- Taxon
- mulberrye1
- Treatment
- Growth hormone TDZ supplemented in the mediae2
- Outcome
- callus formation and growthe2
- Direction
- inducee2
Not reportedComparator
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e2“Growth hormone TDZ supplemented in the media could induce callus formation and growth but inhibited bud growth”
Claim · model-written summary
TDZ in the medium inhibited mulberry bud growth.
- Taxon
- mulberrye1
- Treatment
- Growth hormone TDZ supplemented in the mediae2
- Outcome
- bud growthe2
- Direction
- inhibitede2
Not reportedComparator
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e2“Growth hormone TDZ supplemented in the media could induce callus formation and growth but inhibited bud growth”
Claim · model-written summary
6-BA in the medium promoted mulberry winter bud regeneration.
- Taxon
- mulberrye1
- Treatment
- Cytokinin 6-BA supplemented in the mediae3
- Outcome
- bud regeneratione3
- Direction
- promotede3
Not reportedComparator
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e3“Cytokinin 6-BA supplemented in the media promoted bud regeneration”
Claim · model-written summary
The winter bud regeneration rate on 3.0 mg/L 6-BA was significantly higher than on 1.0 mg/L 6-BA.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e4“the rate of winter bud regeneration on medium containing 3.0 mg/L 6-BA was significantly higher than that on medium containing 1.0 mg/L 6-BA”
Claim · model-written summary
Xinjiang Yaosang had the highest winter bud regeneration rate of 66.7% among the 8 germplasm resources on 3.0 mg/L 6-BA.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e5“Xinjiang Yaosang and Xiaohuaye Pisang had the highest inducing rate of clustered buds with winter bud regeneration rate up to 66.7%”
e6“Among the 8 mulberry germplasm resources whose winter buds were cultured on media containing 3.0 mg/L 6-BA”
Claim · model-written summary
Xiaohuaye Pisang had the highest winter bud regeneration rate of 66.7% among the 8 germplasm resources on 3.0 mg/L 6-BA.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e5“Xinjiang Yaosang and Xiaohuaye Pisang had the highest inducing rate of clustered buds with winter bud regeneration rate up to 66.7%”
e6“Among the 8 mulberry germplasm resources whose winter buds were cultured on media containing 3.0 mg/L 6-BA”
Claim · model-written summary
Diansang took the second place with a winter bud regeneration rate of 55.5% on 3.0 mg/L 6-BA.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e6“Among the 8 mulberry germplasm resources whose winter buds were cultured on media containing 3.0 mg/L 6-BA”
e7“Diansang took the second place with winter bud regeneration rate of 55.5%”
Claim · model-written summary
Tianmushan Sang had a winter bud regeneration rate of 40.0% on 3.0 mg/L 6-BA.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e6“Among the 8 mulberry germplasm resources whose winter buds were cultured on media containing 3.0 mg/L 6-BA”
e0“Tianmushan Sang and Sri Lanka had winter bud regeneration rate of 40.0% and 25.0% respectively”
Claim · model-written summary
Sri Lanka had a winter bud regeneration rate of 25.0% on 3.0 mg/L 6-BA.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e6“Among the 8 mulberry germplasm resources whose winter buds were cultured on media containing 3.0 mg/L 6-BA”
e0“Tianmushan Sang and Sri Lanka had winter bud regeneration rate of 40.0% and 25.0% respectively”
Claim · model-written summary
Xinjiang Yaosang shoots formed roots at an induction rate of 20.0% one month after transfer to rooting medium.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e0“only shoots of Xinjiang Yaosang and Diansang formed root system (the induction rate was 20.0% and 14.3% respectively) and survived after being transplanted into soil”
Claim · model-written summary
Diansang shoots formed roots at an induction rate of 14.3% one month after transfer to rooting medium.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e0“only shoots of Xinjiang Yaosang and Diansang formed root system (the induction rate was 20.0% and 14.3% respectively) and survived after being transplanted into soil”
Claim · model-written summary
Xinjiang Yaosang shoots survived after being transplanted into soil.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e0“only shoots of Xinjiang Yaosang and Diansang formed root system (the induction rate was 20.0% and 14.3% respectively) and survived after being transplanted into soil”
Claim · model-written summary
Diansang shoots survived after being transplanted into soil.
Verbatim
e1“the winter buds of tested mulberry germplasm resources stopped growing on media not supplemented with growth regulators”
e0“only shoots of Xinjiang Yaosang and Diansang formed root system (the induction rate was 20.0% and 14.3% respectively) and survived after being transplanted into soil”
Claim · model-written summary
In vitro tissue culture and plant regeneration is an effective method to conserve and utilize rare and endangered mulberry germplasm resources.
Verbatim
e0“in vitro tissue culture and plant regeneration is an effective method to conserve and utilize rare and endangered mulberry germplasm resources such as Diansang and Xinjiang Yaosang”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.